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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 14,414 results for: TIM;

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Showing: 11701 - 11720 of 14414 results

  1. pACT2-CAS9c

    Plasmid
    #51055
    Purpose
    Express Eukaryotic-codon-optimized Cas9c gene in yeast under ADH1 promoter for genome editing. SV40 NLS is fused to the C terminal. GAL4 region from pACT2 is removed.
    Depositor
    Yunde Zhao
    Article
    Gao et al J Integr Plant Biol. 2013 Dec 30. doi: 1
    Insert
    Cas9c
    Use
    CRISPR
    Tags
    SV40 NLS
    Expression
    Bacterial and Yeast
    Promoter
    yeast ADH1 promoter
    Available Since
    Feb. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    51055-plasmid-map-sequence-id-77327

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  2. VRQR variant

    Plasmid
    #138562
    Purpose
    Expresses human codon-optimized SpCas9-VRQR and blasticidin resistance: EFS promoter-VRQR-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133
    Insert
    VRQR
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    D1135V, G1218R, R1335Q, T1337R
    Promoter
    EFS
    Available Since
    June 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    138562-plasmid-map-sequence-id-274786

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  3. RH5-bio

    Plasmid
    #47780
    Purpose
    Expresses enzymatically monobiotinylated full-length RH5 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Gavin Wright
    Article
    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.
    Insert
    Codon-optimised RH5
    Tags
    enzymatic biotinylation sequence and rat Cd4 d3+4
    Expression
    Mammalian
    Mutation
    Exogenous signal peptide of mouse origin, changed…
    Promoter
    CMV
    Available Since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    47780-plasmid-map-sequence-id-68799

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  5. pACDura3

    Plasmid
    #138711
    Purpose
    Expresses group II intron Ll.LtrB and IEP (LtrA) from Lactoccus lactis under control of T7 promoter. Ll.LtrB is retargeted to insert into 635s locus of LacZ gene and ura3 gene is cloned inside.
    Depositor
    Víctor de Lorenzo
    Article
    Velazquez et al ACS Synth Biol. 2019 Sep 20;8(9):2186-21
    Insert
    ura3 gene (URA3 Budding Yeast)
    Expression
    Bacterial
    Mutation
    optimized ura3 gene for expression in Escherichia…
    Promoter
    pEM7 promoter
    Available Since
    Sept. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    138711-plasmid-map-sequence-id-311288

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  7. LiPOP1-NanoLuc

    Plasmid
    #168265
    Purpose
    Chemo-optogenetic control of necroptotic cell death by coupling LiPOP1 with NanoLuc-aided bioluminescence optogenetic stimulation (NanoLOGS)
    Depositor
    Yubin Zhou
    Article
    He et al Adv Sci (Weinh). 2021 May 6;8(13):210042
    Insert
    MLKL(1-125) (MLKL Human)
    Tags
    mCherry, NanoLuc, CRY2-PHR
    Expression
    Mammalian
    Mutation
    H15A, K16A, R17A
    Promoter
    CMV
    Available Since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    168265-plasmid-map-sequence-id-334508

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  9. SpCas9-NRTH

    Plasmid
    #198541
    Purpose
    Expresses human codon-optimized SpCas9-NRTH and blasticidin resistance: EFS promoter-SpCas9-NRTH-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.
    Insert
    SpCas9-NRTH
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Promoter
    EFS
    Available Since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    198541-plasmid-map-sequence-id-388886

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  11. pCRISPReporter-mCherry

    Plasmid
    #65008
    Purpose
    E. coli reporter vector with MCS for inserting bacterial promoter region through 5' end of gene of interest, upstream and in-frame with mCherry reporter.
    Depositor
    Mattheos Koffas
    Article
    Cress et al ACS Synth Biol. 2015 Mar 30.
    Insert
    mCherry
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    User-defined
    Available Since
    May 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    65008-plasmid-map-sequence-id-109895

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  13. P2RY11-DuET

    Plasmid
    #213364
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    P2RY11 (P2RY11 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213364-plasmid-map-sequence-id-430884

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  14. SpCas9-NG-CGBE1

    Plasmid
    #198537
    Purpose
    Expresses human codon-optimized SpCas9-NG-CGBE1 and blasticidin resistance: EFS promoter-SpCas9-NG-CGBE1-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.
    Insert
    SpCas9-NG-CGBE1
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Promoter
    EFS
    Available Since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    198537-plasmid-map-sequence-id-388909

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  20. Sniper-Cas9

    Plasmid
    #138559
    Purpose
    Expresses human codon-optimized Sniper-SpCas9 and blasticidin resistance: EFS promoter-Sniper-Cas9-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133
    Insert
    Sniper-Cas9
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    F539S, M763I, K890N
    Promoter
    EFS
    Available Since
    June 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    138559-plasmid-map-sequence-id-298901

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Showing: 11701 - 11720 of 14414 results