We narrowed to 64,754 results for: cell;

1. pCAG/hArf4(WT)-EGFP

   Plasmid

   #79407

   Purpose

   Expresses C-terminally EGFP-tagged Arf4(WT) in mammalian cells

   Depositor

   Kazuhisa Nakayama

   Article

   Nakai et al Mol Biol Cell. 2013 Aug;24(16):2570-81.

   Insert

   Arf4 (ARF4 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   April 12, 2017

   Availability

   Academic Institutions and Nonprofits only

2. pUCHR-CD5Flag2-P2A-smAID-mClover

   Plasmid

   #110657

   Purpose

   Lentiviral vector expressing cell surface Flag-tag and monomeric GFP N-terminaly fused with auxin inducible degron

   Depositor

   Dmitriy Mazurov

   Article

   Zotova et al Sci Rep. 2019 Feb 28;9(1):3132. doi: 10.

   Insert

   CD5Flag2-P2A-AID-mClover
   

   Use

   Lentiviral

   Promoter

   CMV

   Available Since

   March 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pFX-Lifeact-Venus

   Plasmid

   #174478

   Purpose

   Express Venus with actin-targeting sequence in mammalian cells

   Depositor

   Yusuke Ohba

   Article

   Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

   Insert

   Venus with actin-targeting sequence
   

   Tags

   Lifeact (N-terminal 17 amino acids from S. Cerevi…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3-FLAG/MKRN1(1-369)

   Plasmid

   #78753

   Purpose

   To overexpress MKRN1(1-369) in Mammalian Cells

   Depositor

   Jaewhan Song

   Article

   Lee et al EMBO J. 2009 Jul 22;28(14):2100-13. doi:

   Insert

   MKRN1 (MKRN1 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   1-369

   Promoter

   CMV

   Available Since

   June 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pFX-Venus-KRasCT

   Plasmid

   #175834

   Purpose

   Express Venus with plasma membrane-targeting sequence in mammalian cells

   Depositor

   Yusuke Ohba

   Article

   Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

   Insert

   Venus with plasma membrane-targeting sequence
   

   Tags

   C-terminal hypervariable region of K-Ras 4B

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGFP-Ub-VPS13D_dC

   Plasmid

   #176466

   Purpose

   express VPS13D lacking C-terminal 407 aa in mammalian cells

   Depositor

   Richard Youle

   Article

   Baldwin et al J Cell Biol. 2021 May 3;220(5). pii: 212

   Insert

   VPS13D (VPS13D Human)
   

   Tags

   GFP-Ub

   Expression

   Mammalian

   Mutation

   C-terminal 407 aa is deleted.

   Promoter

   CMV

   Available Since

   Oct. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. LB1361 (Bcl-2 promoter with MHS12)

   Plasmid

   #15391

   Depositor

   Linda Boxer

   Article

   Duan et al Oncogene. 2007 Apr 19. 26(18):2635-41.

   Insert

   B-cell lymphoma/leukemia-2 (BCL2 Human)
   

   Use

   Luciferase

   Tags

   Bcl-2-Luciferase

   Expression

   Bacterial and Mammalian

   Mutation

   Bcl-2 full promoter with MHS12

   Available Since

   Aug. 1, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3-Xl-VSP1 R152Q GFP

   Plasmid

   #51884

   Purpose

   expression of Xl-VSP1 mutant fused to GFP in Xenopus oocytes or mammalian cells

   Depositor

   Laurinda Jaffe

   Article

   Ratzan et al J Cell Physiol. 2011 Nov;226(11):2740-6.

   Insert

   VSP1 R152Q (tpte2 Frog)
   

   Use

   Xenopus oocyte expression

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   R152Q: near the extracellular side of the fourth …

   Promoter

   CMV

   Available Since

   March 13, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. ATP1A1_G3_RUNX1_Nick-38_Dual_sgRNA

   Plasmid

   #173214

   Purpose

   Control vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G3 and RUNX1 nick-38 sgRNAs from two independent U6 promoters.

   Depositor

   Yannick Doyon

   Article

   Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

   Insert

   ATP1A1 G3 sgRNA + RUNX1 Nick-38 sgRNA
   

   Use

   CRISPR; Prime editing (pe3)

   Expression

   Mammalian

   Promoter

   Tandem U6 promoters

   Available Since

   Nov. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pFUW-tetO-Nfat1

    Plasmid

    #139830

    Purpose

    doxycycline-inducible expression of mouse Nfat1 in mammalian cells

    Depositor

    Filipe Pereira

    Article

    Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

    Insert

    Nfat1
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    TRE-mCMV

    Available Since

    April 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. PR594-His-bio

    Plasmid

    #52332

    Purpose

    Expresses full-length Neuron-specific protein family member 1 ectodomain in mammalian cells. N-terminal His tag, biotinylation sequence and rat Cd4d3+4 tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    PR594 (NSG1 Human)
    

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSN65

    Plasmid

    #100123

    Purpose

    expresses putative SOX2 DBD with a V5 tag

    Depositor

    Mark Ptashne

    Article

    Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

    Insert

    mCitrine-P2A-putative SOX2 DBD (41-109) with a V5 tag (SOX2 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Sept. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. myc-UVRAG S498D

    Plasmid

    #86739

    Purpose

    N-terminal myc-tagged protein expression in mammalian cells

    Depositor

    Do-Hyung Kim

    Article

    Kim et al Mol Cell. 2015 Jan 22;57(2):207-18. doi:

    Insert

    human UVRAG (UVRAG Human)
    

    Tags

    myc

    Expression

    Mammalian

    Mutation

    S498D

    Promoter

    CMV

    Available Since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. FKBP-GTSE1(638-720)-GFP

    Plasmid

    #100735

    Purpose

    To express a clathrin "hook" in mammalian cells to hot-wire endocytosis

    Depositor

    Stephen Royle

    Article

    Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

    Insert

    GTSE1 (GTSE1 Human)
    

    Tags

    FKBP and GFP

    Expression

    Mammalian

    Mutation

    C-terminus only

    Promoter

    CMV

    Available Since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBI-CMV1-mir451-CMV2-mir1~144

    Plasmid

    #164665

    Purpose

    Express miR-451 and miR-1-1~144 in mammalian cells

    Depositor

    David Bartel

    Article

    Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

    Inserts

    miR-451a (MIR451A Human)
    miR-1-1~144 (MIR1-1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSN16

    Plasmid

    #100103

    Purpose

    expresses a chimeric OCT4-activator fusion

    Depositor

    Mark Ptashne

    Article

    Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

    Insert

    vexGFP-P2A-OCT4 fused to a 'strong' VP16 domain (POU5F1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Nov. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. mEmerald-PCNA-19-NLS-4

    Plasmid

    #54222

    Purpose

    Localization: Nucleus, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Article

    Lu et al J Cell Biol. 2010 Jun 14;189(6):967-80.

    Insert

    PCNA-19 NLS (PCNA Human)
    

    Tags

    SV40NLS and mEmerald

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 16, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

18. pDEST-CMV GFP-GABARAPL1 G116A

    Plasmid

    #123118

    Purpose

    Expresses EGFP-GABARAPL1 G116A in mammalian cells.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Gamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Glycine 116 to Alanine

    Promoter

    CMV

    Available Since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEF Itk K390R myc

    Plasmid

    #20707

    Depositor

    Leslie Berg

    Article

    Bunnell et al J Biol Chem. 2000 Jan 21. 275(3):2219-30

    Insert

    Itk K390R (Itk Mouse)
    

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Kinase Dead. K390R.

    Available Since

    April 28, 2009

    Availability

    Academic Institutions and Nonprofits only

20. pClneoFH-let60 G12V

    Plasmid

    #66860

    Purpose

    Express FLAG-His6-tagged dominant active let60, in which Glycine 12 is mutated to Valine, in mammalian cells

    Depositor

    Yutaka Hata

    Article

    Iwasa et al Exp Cell Res. 2013 Feb 1;319(3):1-11. do

    Insert

    Let-60 (let-60 Nematode)
    

    Tags

    FLAG-His6

    Expression

    Mammalian

    Mutation

    Glycine 12 is mutated to valine

    Promoter

    CMV

    Available Since

    Aug. 17, 2015

    Availability

    Academic Institutions and Nonprofits only