We narrowed to 11,577 results for: 112

1. pEGFP-N1-mSNX27-H112A RNAi-resistant

   Plasmid

   #163621

   Purpose

   mouse SNX27 cDNA with point mutation to resist siRNA against SNX27 and with a point mutation H112A C-terminally tagged with GFP

   Depositor

   Josef Kittler

   Article

   Halff et al Cell Rep. 2019 Nov 26;29(9):2599-2607.e6

   Insert

   mouse SNX27-H112A RNAi-resistant (Snx27 Mouse)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   H112A and RNAi resistance

   Promoter

   Available since

   Jan. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. PF3D7_1123500-COMP-blac-flag-his

   Plasmid

   #110987

   Purpose

   Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.

   Depositor

   Gavin Wright

   Article

   Knockel et al Mol Cell Proteomics. 2021;20:100038. doi

   Insert

   conserved Plasmodium protein (PF3D7_1123500 Synthetic)
   

   Use

   Tags

   Exogenous signal peptide of mouse origin and rat …

   Expression

   Mammalian

   Mutation

   All NXT/S sites mutated to NXA, where X is any am…

   Promoter

   CMV

   Available since

   Oct. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-Tag/E112K without 5'UTR

   Plasmid

   #87724

   Purpose

   Expresses E112K spastin M1 and M87 in mammalian cells

   Depositor

   Peter Baas

   Article

   Solowska et al Hum Mol Genet. 2010 Jul 15;19(14):2767-7

   Insert

   SPAST (SPAST Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   E112K; 5'UTR deleted (1-190 bp)

   Promoter

   CMV

   Available since

   March 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTYB-pLAC-L108S/L109S/F112S

   Plasmid

   #48730

   Purpose

   Bacterial expression of Lacritin Protein with Mutation at proteins 108 and 109 Leucine to Serine and 112 from phenylalanine to serine

   Depositor

   Gordon Laurie

   Article

   Wang et al J Biol Chem. 2013 Jun 21;288(25):18146-6

   Insert

   Lacritin (LACRT Human)
   

   Use

   Tags

   Intein

   Expression

   Bacterial

   Mutation

   Leucine 108 and 109 to Serine; phenylalanine 112 …

   Promoter

   T7

   Available since

   Oct. 28, 2013

   Availability

   Academic Institutions and Nonprofits only

5. pcDNA5/FRT/TO-Venus-Flag-Gateway (1124)

   Plasmid

   #40999

   Depositor

   Jonathon Pines

   Article

   Di Fiore et al J Cell Biol. 2010 Aug 23;190(4):501-9.

   Type

   Empty backbone

   Use

   Tags

   3xFLAG (DYKDHDGDYKDHDIDYKDDDDK) and Venus YFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 8, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pShHELIX(Cas12k-TniQ-TniQ)-sgRNA_entry (CJT112)

   Plasmid

   #181791

   Purpose

   Expresses 3-component ShHELIX containing a Cas12k-TniQ-TniQ fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

   Depositor

   Benjamin Kleinstiver

   Article

   Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   nAniI-ShTnsB, ShTnsC, ShCas12k-ShTniQ-ShTniQ
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   nAniI = K227M, F80K, L232K

   Promoter

   Lac and J23119

   Available since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. (112) pcDNA3.1-GFP-Flag-Nup62-EPEA

   Plasmid

   #168101

   Purpose

   GFP and Flag tagged Nup62 with EPEA nanobody tag

   Depositor

   Christina Woo

   Article

   Ge et al Nat Chem Biol. 2021 Mar 8. pii: 10.1038/

   Insert

   GFP-Flag-Nup62-EPEA
   

   Use

   Tags

   EPEA, Flag, and GFP

   Expression

   Mammalian

   Mutation

   Promoter

   T7/CMV

   Available since

   May 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDF0582 huDisCas7-11 R1045-GGGS-R1122 U6-NT guide

   Plasmid

   #186991

   Purpose

   AAV transgene plasmid for Cas7-11-R1045-GGGS-R1122, with U6 promoter-driven expression of non-targeting crRNA guide

   Depositor

   Jonathan Gootenberg, Omar Abudayyeh

   Article

   Kato et al Cell. 2022 May 25. pii: S0092-8674(22)00

   Insert

   cas7-11-r1045-gggs-r1122, non-targeting crRNA guide
   

   Use

   AAV

   Tags

   Expression

   Mutation

   r1045-gggs-r1122 deletion

   Promoter

   Available since

   Sept. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. 1129 pcDNA3 GFP FKHR H215R AAA

   Plasmid

   #9034

   Depositor

   William Sellers

   Article

   Nakamura et al Mol Cell Biol. 2000 Dec . 20(23):8969-82

   Insert

   FKHR H215R AAA (FOXO1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   H215R T24A S256A S319A

   Promoter

   Available since

   April 20, 2006

   Availability

   Academic Institutions and Nonprofits only

10. pDF0385 pcmv-hudiscas7-11-r1045-gggs-r1122

    Plasmid

    #186982

    Purpose

    mammalian expression of cas7-11-r1045-gggs-r1122

    Depositor

    Jonathan Gootenberg, Omar Abudayyeh

    Article

    Kato et al Cell. 2022 May 25. pii: S0092-8674(22)00

    Insert

    cas7-11-r1045-gggs-r1122
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    r1045-gggs-r1122 deletion

    Promoter

    Available since

    Aug. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA5 FRT TO myc Separase delta 55/S1126A

    Plasmid

    #59825

    Purpose

    Allows the integration of myc Separase delta 55/S1126A in the genome and Tet-inducible expression.

    Depositor

    Stephen Taylor

    Article

    Holland et al J Biol Chem. 2007 Aug 24;282(34):24623-3

    Insert

    Separase (ESPL1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    delta 55/S1126A

    Promoter

    hybrid human cytomegalovirus (CMV)/TetO2 promoter

    Available since

    May 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. N112 nLV EF-1a-PGK-Puro Gal4-HP1a CS

    Plasmid

    #43969

    Depositor

    Jerry Crabtree

    Article

    Hathaway et al Cell. 2012 Jun 22;149(7):1447-60. doi: 1

    Insert

    Cbx5 (Cbx5 Mouse)
    

    Use

    Lentiviral

    Tags

    Gal4/DBD

    Expression

    Mutation

    N-term chromo domain removed

    Promoter

    EF-1a

    Available since

    June 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. 3226 pSFFV-neo Bad S112A S136A

    Plasmid

    #8760

    Depositor

    Stanley Korsmeyer

    Article

    Harada et al Proc Natl Acad Sci U S A 2001 Aug 14;98(

    Insert

    Bad (Bad Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S112A, S136A

    Promoter

    Available since

    July 18, 2006

    Availability

    Academic Institutions and Nonprofits only

14. pCMV5-Flag-EPLIN alpha delta N112

    Plasmid

    #40935

    Depositor

    Elizabeth Luna, Scott Maul

    Article

    Song et al Mol Biol Cell. 2002 Apr;13(4):1408-16.

    Insert

    EPLIN (LIMA1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    deleted amino acids 1-112

    Promoter

    Available since

    Dec. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

15. 3603 pGEX4T-1 Bad S112A (104-141)

    Plasmid

    #8802

    Depositor

    Stanley Korsmeyer

    Article

    Harada et al Mol Cell 1999 Apr;3(4):413-22.

    Insert

    Bad (Bad Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Contains AA's 104-141; S112A

    Promoter

    Ptac

    Available since

    July 6, 2005

    Availability

    Academic Institutions and Nonprofits only

16. pT7-V5-SBP-C1-HsUpf1_115-1129-S1107A-siRNAres_S

    Plasmid

    #147477

    Purpose

    Mammalian Expression of HsUpf1_115-1129-S1107A-siRNAres

    Depositor

    Elisa Izaurralde

    Article

    Jonas et al Genes Dev. 2013 Jan 15;27(2):211-25. doi

    Insert

    HsUpf1_115-1129-S1107A-siRNAres (UPF1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    4 non silent mutations E167G, K282R, A442G and T1…

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAc5.1B-lambdaN-HA-DmUPF1-S1121A-siRNAres-V5His6_N

    Plasmid

    #146992

    Purpose

    Insect Expression of DmUPF1-S1121A-siRNAres

    Depositor

    Elisa Izaurralde

    Insert

    DmUPF1-S1121A-siRNAres (Upf1 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    1 non silent mutations: G995E compared to the seq…

    Promoter

    Available since

    Jan. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAc5.1B-lambdaN-HA-DmUPF1-S1140AS1146AS1121A-siRNAres-V5His6_N

    Plasmid

    #146993

    Purpose

    Insect Expression of DmUPF1-S1140AS1146AS1121A-siRNAres

    Depositor

    Elisa Izaurralde

    Insert

    DmUPF1-S1140AS1146AS1121A-siRNAres (Upf1 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    1 non silent mutations: G995E compared to the seq…

    Promoter

    Available since

    Jan. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDF0112 cmv-cluciferase-polya-ef1a-g-luciferase-polya

    Plasmid

    #186980

    Purpose

    mammalian expression of Cluc-Gluc reporter

    Depositor

    Jonathan Gootenberg, Omar Abudayyeh

    Article

    Kato et al Cell. 2022 May 25. pii: S0092-8674(22)00

    Insert

    Cluc, Gluc
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSLQ11296: pHR-HSP-hyperdCas12a-miniVPR-EPS-puro

    Plasmid

    #210604

    Purpose

    heat shock promoter - activated hyperdCas12a-based transcriptional activator

    Depositor

    Stanley Qi

    Article

    Liu et al Nat Commun. 2023 Oct 18;14(1):6575. doi:

    Insert

    hyperdCas12a-miniVPR
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    truncated hsp70

    Available since

    Dec. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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