We narrowed to 23,563 results for: CHI

1. pGEX Sck-SH2

   Plasmid

   #46478

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   Sck/ShcB (Shc2 Mouse)
   

   Use

   Tags

   GST and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 464-573

   Promoter

   Tac

   Available since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEX Socs7-SH2

   Plasmid

   #46509

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   Socs7 (SOCS4 Human)
   

   Use

   Tags

   AviTag, GST, and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 280-386

   Promoter

   Tac

   Available since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEX Nsp3-SH2

   Plasmid

   #46461

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   Nsp3 (SH2D3C Human)
   

   Use

   Tags

   GST and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 212-330

   Promoter

   Tac

   Available since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. CMYA5T2/pDEST15

   Plasmid

   #98109

   Purpose

   Express CMYA5 (aa 4039 – 4069) Leu allele (4063) with a N-terminal GST tag in bacteria

   Depositor

   Rita Shiang

   Article

   Hsiung et al J Psychiatr Res. 2019 Apr;111:8-15. doi:

   Insert

   CMYA5 (CMYA5 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   rs10043986 (NP_705838.3:p.Pro4063Leu)

   Promoter

   Available since

   Aug. 21, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pET21b-effector-ncRNA-rrt-His8

   Plasmid

   #194089

   Purpose

   Expresses Ec86 operon (effector, ncRNA and reverse transcriptase) in Escherichia coli

   Depositor

   Tingting Zou

   Article

   Wang et al Nat Microbiol. 2022 Sep;7(9):1480-1489.

   Insert

   Ec86 effector-ncRNA-rrt
   

   Use

   Tags

   His8

   Expression

   Mutation

   Promoter

   T7

   Available since

   May 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pYG215

   Plasmid

   #200837

   Purpose

   Transcribes a glmZ' (1-146)-gfp fusion. gfp can be replaced via AgeI/XbaI-sites with gene of interest to release its RNA with a 5' monophosphate upon cleavage.

   Depositor

   Boris Görke

   Article

   Schilder et al Nucleic Acids Res. 2023 Mar 29:gkad226.

   Insert

   glmZ (glmZ Escherichia coli str. K-12 substr. MG1655)
   

   Use

   Synthetic Biology

   Tags

   GFP

   Expression

   Bacterial

   Mutation

   Promoter

   P-LlacO-1

   Available since

   Aug. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTV-Flnc-5prime-1000-24MS96T-3F_NeoR

   Plasmid

   #174881

   Purpose

   Targeting vector for generating Flnc STREAMING-tag KI cell

   Depositor

   Hiroshi Ochiai

   Article

   Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

   Insert

   STREAMING-tag with homology arms for Flnc
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTV-Usp5-5prime-1000-24MS96T-3F_NeoR

   Plasmid

   #174885

   Purpose

   Targeting vector for generating Usp5 STREAMING-tag KI cell

   Depositor

   Hiroshi Ochiai

   Article

   Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

   Insert

   STREAMING-tag with homology arms for Usp5
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTV-Wnk1-5prime-1000-24MS96T-3F_NeoR

   Plasmid

   #174886

   Purpose

   Targeting vector for generating Wnk1 STREAMING-tag KI cell

   Depositor

   Hiroshi Ochiai

   Article

   Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

   Insert

   STREAMING-tag with homology arms for Wnk1
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTS2334_Tier3(Lenti_inverse)-rtTA-YPet_Puro

    Plasmid

    #169665

    Purpose

    Tier-3 vector for lentiviral stable integration of the rtTA and puromyine - Ypet selection cassette in inverted direction with polyA (PRPBSA-YPet-p2a-PuroR::PPGK-rtTA-pA::A3-pA)

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PPGK-driven rtTA expression and PRPBSA-driven YPet and PuroR expression
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV / PPGK / RPBSA

    Available since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTS2333_Tier3(Lenti_inverse)-YPet_Puro

    Plasmid

    #169664

    Purpose

    Tier-3 vector for stable integration via lentiviral transduction in inverted direction with polyA carrying a constitutive expression cassette for puromyine and Ypet (PCMV-5'LTR-Psi-gag-env-PRPBSA-YPet-p2A-PuroR-pA::A2-pA::A3-pA-WPRE-3'LTR)

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PRPBSA-driven YPet and PuroR expression
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV / RPBSA

    Available since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJJGL003

    Plasmid

    #180607

    Purpose

    Plasmid expressing E. coli codon optimized His-MBP-TEV-DpbCasX+Region3 insertion

    Depositor

    Jennifer Doudna

    Article

    Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6

    Insert

    DpbCasX with R3 loop
    

    Use

    CRISPR

    Tags

    10x His and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSB1C3_mTq-Tat (c-plasmid)

    Plasmid

    #127635

    Purpose

    Encodes a fluorecent protein with an RNA binding peptide: mTurquoise2-tat.Expression with constitutive E. coli RNAP promoter (J23106), ribozyme PlmJ and RBS BBa_B0034.

    Depositor

    Friedrich Simmel

    Article

    Schwarz-Schilling et al Nano Lett. 2018 Apr 11;18(4):2650-2657.

    Insert

    mTurquoise2
    

    Use

    Synthetic Biology

    Tags

    RNA binding peptide: PCP and RNA binding peptide:…

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET29a-hNABP1NhNABP2C

    Plasmid

    #128312

    Purpose

    Express chimeric protein hNABP1 N-terminus and hNABP2 C-terminus in E. coli with a C-terminal His-tag

    Depositor

    Yuliang Wu

    Article

    Vidhyasagar et al Biochim Biophys Acta. 2016 Feb;1860(2):3

    Insert

    chimeric protein hNABP1N+hNABP2C (NABP1, NABP2 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    chimeric protein hNABP1N+hNABP2C

    Promoter

    T7

    Available since

    Nov. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET22b-ecDHFR-dCys-T46C

    Plasmid

    #110543

    Purpose

    Bacterial expression of E.coli DHFR C85A/C152S/T46C mutant

    Depositor

    Stephen Benkovic

    Article

    Liu et al J Am Chem Soc. 2014 Jul 23;136(29):10349

    Insert

    ecDHFR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    T46 mutated to C46, C85 mutated to A85, C152 muta…

    Promoter

    T7

    Available since

    June 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET22b-ecDHFR-dCys-L54C

    Plasmid

    #110542

    Purpose

    Bacterial expression of E.coli DHFR C85A/C152S/L54C mutant

    Depositor

    Stephen Benkovic

    Article

    Liu et al J Am Chem Soc. 2014 Jul 23;136(29):10349

    Insert

    ecDHFR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    L54 mutated to C54, C85 mutated to A85, C152 muta…

    Promoter

    T7

    Available since

    June 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET22b-ecDHFR-D27S/Y100F

    Plasmid

    #110827

    Purpose

    Bacterial expression of E.coli DHFR D27S/Y100F mutant

    Depositor

    Stephen Benkovic

    Article

    Liu et al Proc Natl Acad Sci U S A. 2014 Dec 23;11

    Insert

    ecDHFR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D27 mutated to S27, Y100 mutated to F100

    Promoter

    T7

    Available since

    June 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. CMYA5T2/pDEST17

    Plasmid

    #98118

    Purpose

    Express CMYA5 (aa 4039 – 4069) Leu allele (4063) witha N-terminal 6x-His tag in bacteria

    Depositor

    Rita Shiang

    Article

    Hsiung et al J Psychiatr Res. 2019 Apr;111:8-15. doi:

    Insert

    CMYA5 (CMYA5 Human)
    

    Use

    Tags

    6X-His

    Expression

    Bacterial

    Mutation

    rs10043986 (NP_705838.3:p.Pro4063Leu)

    Promoter

    Available since

    Aug. 21, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. CMYA5T/pACT2

    Plasmid

    #97209

    Purpose

    CMYA5 (aa 4039 – 4069) Leu allele (4063) in a GAL4 AD vector, used for yeast-two hybrid

    Depositor

    Rita Shiang

    Article

    Hsiung et al J Psychiatr Res. 2019 Apr;111:8-15. doi:

    Insert

    CMYA5 (CMYA5 Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    rs10043986 (NP_705838.3:p.Pro4063Leu)

    Promoter

    Available since

    Aug. 21, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pNC2

    Plasmid

    #72438

    Purpose

    Produces Escherichia coli N5-carboxyaminoimidazole ribonucleotide mutase (EcPurE1); NCAIR synthetase (EcPurK)

    Depositor

    T. J. Kappock, JoAnne Stubbe

    Article

    Meyer et al Biochemistry. 1999 Mar 9;38(10):3012-8.

    Inserts

    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Pro64 to Arg; Asp65 to His; Gln206 to Arg

    Promoter

    T7 and n/a

    Available since

    May 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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