We narrowed to 6,401 results for: cas9 expression plasmid

1. PZac2.1 gfaABC1D-NLS-SaCas9-3xHA-NLS bGH

   Plasmid

   #178960

   Purpose

   Expresses saCas9 protein specifically in astrocytes

   Depositor

   Baljit Khakh

   Article

   Endo et al Science. 2022 Nov 4;378(6619):eadc9020.

   Insert

   Staphylococcus aureus Cas9 (SaCas9)
   

   Use

   AAV

   Tags

   3x HA

   Expression

   Mutation

   Promoter

   gfaABC1D (astrocyte specific)

   Available since

   Nov. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSimpleII-U6-tracr-U6-crRNA(tdTomato)-NLS-NmCas9-HA-NLS(s)

   Plasmid

   #47869

   Purpose

   All-in-one plasmid targeting tdTomato

   Depositor

   Erik Sontheimer, James Thomson

   Article

   Hou et al Proc Natl Acad Sci U S A. 2013 Aug 12.

   Inserts

   NmCas9
   U6pr-tracrRNA
   tdTomato crRNA
   

   Use

   CRISPR

   Tags

   HA and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-pCALM1-sFLEx-HA-SpCas9-miniU6-sgRNAShank3

   Plasmid

   #213969

   Purpose

   AAV vector for encoding SpCas9 driven by pCALM1 promoter targeting Shank3 locus in the presence of Cre recombinase

   Depositor

   Zhonghua Lu

   Article

   Wang et al Cell Rep. 2023 Nov 28;42(11):113348. doi

   Insert

   Shank3 sgRNA
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV- GfaABC1D::Cas9-HA-sgRNA-Ezr-seq2

   Plasmid

   #240865

   Purpose

   GfaABC1D promoter driven HA-tagged SaCas9 together with U6 driven expression of sgRNA targeting exon-1 of mouse Ezrin; for knocking-out ezrin in murine astrocytes

   Depositor

   Mark Verheijen

   Article

   Badia-Soteras et al Biol Psychiatry. 2023 Aug 1;94(3):226-23

   Insert

   U6 driven sgRNA Targeting exon 1 of EZR (Ezr Mouse)
   

   Use

   AAV and CRISPR

   Tags

   SaCas9 + 3X HA-Tag

   Expression

   Mammalian

   Mutation

   Promoter

   GfaABC1D

   Available since

   Aug. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV- GfaABC1D::Cas9-HA-sgRNA-Ezr-Seq1

   Plasmid

   #240094

   Purpose

   GfaABC1D promoter driven HA-tagged SaCas9 together with U6 driven expression of sgRNA targeting exon-1 of mouse Ezrin; for knocking-out ezrin in murine astrocytes

   Depositor

   Mark Verheijen

   Article

   Badia-Soteras et al Biol Psychiatry. 2023 Aug 1;94(3):226-23

   Insert

   U6 driven sgRNA Targeting exon 1 of EZR (Ezr Mouse)
   

   Use

   AAV and CRISPR

   Tags

   SaCas9 + 3X HA-Tag

   Expression

   Mammalian

   Mutation

   Promoter

   GfaABC1D

   Available since

   Aug. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV hUbC- VP64-dCas9-VP64-T2A- GFP(No LoxP sites)

   Plasmid

   #66708

   Purpose

   expresses VP64-dCas9-VP64, same plasmid as Addgene 59791 but with LoxP sites removed

   Depositor

   Charles Gersbach

   Insert

   S.Pyogenes VP64-dCas9-VP64
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   VP64

   Expression

   Mutation

   D10A and H840A

   Promoter

   hUbC

   Available since

   April 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAV-U6-sgRNA-Scaffold-cTNT-SaCas9-HA-OLLAS

   Plasmid

   #209781

   Purpose

   Expresses SaCas9 by the specific cTNT promoter and sgRNA scaffold by U6 promoter

   Depositor

   Yuxuan Guo

   Article

   Yang et al Circulation. 2024 May 28;149(22):1778-17

   Insert

   cTNT
   

   Use

   AAV

   Tags

   HA and OLLAS

   Expression

   Mutation

   Promoter

   cTNT

   Available since

   May 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAV-U6-msCAMK2d-sgRNA-cTNT-SaCas9-HA-OLLAS

   Plasmid

   #209782

   Purpose

   Expresses SaCas9 by the specific cTNT promoter and sgRNA targeted the mouse Camk2d gene by U6 promoter

   Depositor

   Yuxuan Guo

   Article

   Yang et al Circulation. 2024 May 28;149(22):1778-17

   Insert

   sgRNA
   

   Use

   AAV

   Tags

   HA and OLLAS

   Expression

   Mutation

   Promoter

   cTNT

   Available since

   May 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pOTTC1124 - pAAV MeCP2 SpCas9(D10A)

   Plasmid

   #112719

   Purpose

   An AAV vector that expresses SpCas9 nickase under a neuronal cell promoter

   Depositor

   Brandon Harvey

   Article

   Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

   Insert

   SpCas9
   

   Use

   AAV

   Tags

   Expression

   Mutation

   D10A

   Promoter

   Mecp2

   Available since

   March 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSLPB2B-ecDHFR-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin

    Plasmid

    #187950

    Purpose

    ecDHFR degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.

    Depositor

    Edda Schulz

    Article

    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

    Insert

    ecDHFR-SpdCas9-tagRFPt-P2A-tagBFP
    

    Use

    CRISPR and Synthetic Biology

    Tags

    GGS linker

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSLPB2B-ER50-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin

    Plasmid

    #187948

    Purpose

    ER50 degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.

    Depositor

    Edda Schulz

    Article

    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

    Insert

    ER50-SpdCas9-tagRFPt-P2A-tagBFP
    

    Use

    CRISPR and Synthetic Biology

    Tags

    GGS linker

    Expression

    Mammalian

    Mutation

    6 mutations, T371A, L384M, M421G, G521R, Y537S, N…

    Promoter

    Available since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSLPB2B-FKBP12_F36V-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin

    Plasmid

    #187951

    Purpose

    FKBP12 (F36V mutant) degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.

    Depositor

    Edda Schulz

    Article

    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

    Insert

    FKBP12_F36V-SpdCas9-tagRFPt-P2A-tagBFP
    

    Use

    CRISPR and Synthetic Biology

    Tags

    GGS linker

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSLPB2B-SMASh-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin

    Plasmid

    #187949

    Purpose

    SMASh degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.

    Depositor

    Edda Schulz

    Article

    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

    Insert

    SMASh-SpdCas9-tagRFPt-P2A-tagBFP
    

    Use

    CRISPR and Synthetic Biology

    Tags

    GGS linker

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCSDest2-2XNLS-SpCas9-MT3-NLS-3XHA-NLS-TALentry

    Plasmid

    #69233

    Purpose

    R1335K mutant SpCas9 expression plasmid to clone TALEs through BbsI site (generates compatible overhangs with Acc65I and BamHI sites)

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

    Insert

    SpCas9
    

    Use

    CRISPR

    Tags

    2X NLS, BbsI TALE entry cassette, and NLS-3XHA-NL…

    Expression

    Mammalian

    Mutation

    Arginine 1335 is changed to Lysine

    Promoter

    Available since

    Nov. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSimpleII-U6-tracr-U6-crRNA(OCT4)-NLS-NmCas9-HA-NLS(s)

    Plasmid

    #47870

    Purpose

    All-in-one plasmid targeting human OCT4, cuts roughly ~84bp downstream of stop codon.

    Depositor

    Erik Sontheimer, James Thomson

    Article

    Hou et al Proc Natl Acad Sci U S A. 2013 Aug 12.

    Inserts

    NmCas9
    U6pr-tracrRNA
    OCT4 crRNA
    

    Use

    CRISPR

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. AAV Intein-C-SpyCas9, CMV-AcrIIA4-scaffold (2xBsmBI sites)

    Plasmid

    #120294

    Purpose

    AAV Vector for expression of C-terminal SpyCas9 fragemnt with split-intein and a CMV-driven AcrIIA4 (no miR binding sites)

    Depositor

    Dominik Niopek

    Article

    Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

    Insert

    C-terminal fragment of SpyCas9
    

    Use

    AAV and CRISPR

    Tags

    split-intein

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. CSII-U6-gRNA-CBh-3xFLAG-PA-dCas9-P2A-Puro

    Plasmid

    #83306

    Purpose

    Lentivirus vector to express guideRNA and dCas9 with puro resistant gene

    Depositor

    Tohru Kimura

    Article

    Murakami et al Nat Immunol. 2021 Mar;22(3):301-311. doi

    Insert

    sgRNA and dCas9 from pX330
    

    Use

    Lentiviral

    Tags

    FLAG and PA tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 for sgRNA and CBh for dCas9

    Available since

    Dec. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. lenti U6-sgRNA-acRNA SYN-dCas9-P2A-EGFP

    Plasmid

    #159086

    Purpose

    Expresses dCas9-P2A-EGFP driven by human SYN promoter and empty CRISPR Display sgRNA/accessory RNA from U6 promoter.

    Depositor

    Jeremy Day

    Article

    Carullo et al Nucleic Acids Res. 2020 Aug 18. pii: 589

    Insert

    empty crRNA-acRNA backbone, dCas9-P2A-EGFP
    

    Use

    CRISPR and Lentiviral

    Tags

    EGFP and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    hSYN, U6

    Available since

    Sept. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBK1303-AAV-EFSNC-dCjCas9-MECP2(204-310)

    Plasmid

    #223148

    Purpose

    Expression of truncated MECP2 with dCjCas9 and empty gRNA scaffold

    Depositor

    Boris Kantor

    Article

    Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

    Insert

    MECP2 (MECP2 Human)
    

    Use

    AAV and CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Encodes aa 204-310

    Promoter

    EF1a

    Available since

    Aug. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBK1298-AAV-EFSNC-dCjCas9-HP1bNH(111-173)

    Plasmid

    #223143

    Purpose

    Expression of truncated HP1b with dCjCas9 and empty gRNA scaffold

    Depositor

    Boris Kantor

    Article

    Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

    Insert

    HP1b (CBX1 Human)
    

    Use

    AAV and CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Encodes aa 111-173

    Promoter

    EF1a

    Available since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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