We narrowed to 36,796 results for: KAN

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Cherry-Myo1b

Plasmid

#187366

Purpose

Expresses rat myosin 1b isoform b domain labelled with Cherry

Depositor

Evelyne Coudrier

Article

Iuliano et al J Cell Biol. 2018 Jun 4;217(6):2033-2046

Insert

myosin 1b isoform b (Myo1b Rat)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pN1-RnKIF5C(1-560)-Halo-Flag

Plasmid

#188071

Purpose

Mammalian cell expression of truncated version (aa 1-560) of rat kinesin-1 KIF5C tagged with Halo and Flag tags

Depositor

Kristen Verhey

Article

Budaitis et al Curr Biol. 2022 Jun 6;32(11):2416-2429.e

Insert

rat KIF5C (Kif5c Rat)

Tags

Flag and Halo

Expression

Mammalian

Mutation

aa1-560

Promoter

CMV

Available Since

Aug. 2, 2022

Availability

Academic Institutions and Nonprofits only

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Talin Head

Plasmid

#186140

Purpose

For expression of recombinant Talin head domain in bacteria

Depositor

Michael Rosen

Article

Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

Insert

TLN1 (TLN1 Human)

Tags

His6 (tev cleavage)

Expression

Bacterial

Promoter

Lac

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pET30-T7-sfGFP

Plasmid

#180757

Purpose

pET30 vector missing lacO and N-terminal His tag with sfGFP-S-tag inserted between the Bgl II and T7 terminator

Depositor

Michael Black

Article

Jew et al Front Bioeng Biotechnol. 2022 Jun 23;10:

Insert

superfolder green fluorescent protein

Tags

strep tag

Expression

Bacterial

Available Since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-EGFP-eDHFR(69K6)-MCS

Plasmid

#172100

Purpose

Mammalian expression of a protein of interest fused to the C-terminus of EGFP-eDHFR(69K6)

Depositor

Shinya Tsukiji

Article

Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

Insert

EGFP-eDHFR(69K6)-20aa

Expression

Mammalian

Mutation

eDHFR(69K6): Hexalysine (K6) sequence is inserted…

Promoter

CMV

Available Since

July 14, 2022

Availability

Academic Institutions and Nonprofits only

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pET28b_RanT24N

Plasmid

#186271

Purpose

Expression vector of His-RanT24N in E.coli

Depositor

Masahito Ikawa

Article

Mori et al J Cell Biol. 2021 Oct 4;220(10). pii: 21

Insert

GTP-binding nuclear protein Ran (Ran Mouse)

Tags

His tagged and His-tagged at N terminal

Expression

Bacterial

Mutation

Changed T24 into N

Promoter

T7 promoter

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pJW2341

Plasmid

#186950

Purpose

30aa linker::GFP-C1::IAA7 AID::3xFLAG::10aa linker

Depositor

Jordan Ward

Article

Sepers et al G3 (Bethesda). 2022 Aug 27. pii: 6677660

Insert

30aa linker::GFP-C1::IAA7 AID::3xFLAG::10aa linker

Use

CRISPR

Expression

Worm

Available Since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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GFP-FAK W266A

Plasmid

#186149

Purpose

For expressing FAK in mammalian cells

Depositor

Michael Rosen

Article

Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

Insert

PTK2 (PTK2 Mouse)

Tags

mEGFP

Expression

Mammalian

Promoter

cmv

Available Since

July 1, 2022

Availability

Academic Institutions and Nonprofits only

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GFP-Paxillin DN

Plasmid

#186153

Purpose

For expressing paxillin variants in mammalian cells

Depositor

Michael Rosen

Article

Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

Insert

PXN (PXN Human)

Tags

mEGFP

Expression

Mammalian

Mutation

Paxilln residues 1-321 added to N-terminus of WT …

Promoter

cmv

Available Since

July 1, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP.N3-SLC35A2-GFP

Plasmid

#186284

Purpose

transient expression of C-terminal GFP tagged SLC35A2 isoform c/UGT1 in mammalian cells

Depositor

Somshuvra Mukhopadhyay

Article

Li et al Glycobiology. 2019 Jun 1;29(6):490-503.

Insert

SLC35A2 (SLC35A2 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

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pPL001

Plasmid

#184750

Purpose

Big-IN positive control payload encoding EF1a-driven GFP-T2A-BSD.

Depositor

Jef Boeke

Article

Brosh et al Proc Natl Acad Sci U S A. 2021 Mar 9;118

Insert

pEF1a-eGFP-T2A-BSD-bGHpA

Use

Cre/Lox, Mouse Targeting, and Synthetic Biology ;…

Expression

Mammalian

Promoter

EF1a

Available Since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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rTetR-GFP

Plasmid

#183916

Purpose

Reverse Tet repressor labeled with GFP; binds tetO sites upon doxycycline addition

Depositor

Karsten Rippe

Article

Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

Insert

rTetR-GFP

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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FAM19A2-V5

Plasmid

#184338

Purpose

Expression of V5-tagged mouse FAM19A2

Depositor

Peter Scheiffele

Article

Hauser et al Neuron. 2022 May 8. pii: S0896-6273(22)0

Insert

FAM19A2 (Tafa2 Mouse)

Tags

V5-tag

Expression

Mammalian

Promoter

CMV

Available Since

June 6, 2022

Availability

Academic Institutions and Nonprofits only

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HT-mGold

Plasmid

#183986

Purpose

Expresses fluorescent HaloTag7 fusion protein in mammalian cells in the whole cell.

Depositor

Pablo Rivera-Fuentes

Article

Emmert et al Nat Chem. 2023 Oct;15(10):1415-1421. doi

Insert

HaloTag-mGold

Tags

HaloTag, mGold

Expression

Mammalian

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pgWIZ-SGCAgl

Plasmid

#131456

Purpose

Encodes Alpha Sarcoglycan 3xFLAG tagged protein (Therapeutic gene) to cure LGMD2D and luciferase enzyme for in vivo live imaging

Depositor

Michele Calos

Article

Guha et al Mol Ther Methods Clin Dev. 2019 Oct 14;1

Insert

SGCA (SGCA Human)

Tags

3xFLAG

Expression

Bacterial and Mammalian

Available Since

May 19, 2022

Availability

Academic Institutions and Nonprofits only

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A1-20B1 (P001)

Plasmid

#161820

Purpose

This plasmid carries Yn situ preamplifier A1-20B1 sequence.

Depositor

C. Ron Yu

Article

Wu et al Cell Rep Methods. 2022 Apr 18;2(4):10020

Insert

A1-20B1

Use

Synthetic Biology

Available Since

May 13, 2022

Availability

Academic Institutions and Nonprofits only

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cpLID-micro

Plasmid

#168995

Purpose

circularly permuted LOV2 based optical dimerization system (ssrA-cpLOV2 and sspB)

Depositor

Yubin Zhou

Article

He et al Nat Chem Biol. 2021 Aug;17(8):915-923. d

Insert

mCh-sspB-T2A-cpLID-CAAX

Expression

Mammalian

Promoter

CMV

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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cpLOV2-V5

Plasmid

#168991

Purpose

circularly permuted LOV2 variant-V5

Depositor

Yubin Zhou

Article

He et al Nat Chem Biol. 2021 Aug;17(8):915-923. d

Insert

cpLOV2-V5

Expression

Mammalian

Promoter

CMV

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pET28_mouse-pro-IL1B

Plasmid

#183386

Purpose

Overexpression of catalytic domain of inflammatory caspase in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

Insert

pro-IL1B (Il1b Mouse)

Tags

His-TEV

Expression

Bacterial

Promoter

T7

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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EGFP-IpgD(C439S)

Plasmid

#183667

Purpose

N-terminally tagged Shigella flexneri IpgD (catalytically inactive) for mammalian expression

Depositor

Sergio Grinstein

Article

Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

Insert

IpgD

Tags

EGFP

Expression

Mammalian

Mutation

C439S

Promoter

CMV

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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