We narrowed to 25,312 results for: CHI;

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DpbdCas12e VPR

Plasmid

#188515

Purpose

Expresses FLAG tagged DpbdCas12e VPR

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

dCas12e

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D672A/E769A/D935A

Promoter

EF1a

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_m4_KRAB-MeCP2_Hygro

Plasmid

#167899

Purpose

PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmid

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

dCas9-KRAB-MeCP2

Use

CRISPR

Mutation

D10A, D839A, H840A, N863A

Available Since

Sept. 14, 2022

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_m4_KRAB-MeCP2_EBFP2

Plasmid

#167897

Purpose

PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmid

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

dCas9-KRAB-MeCP2

Use

CRISPR

Mutation

D10A, D839A, H840A, N863A

Available Since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only
NAX_CAGGS_CAS9_m4_KRAB-MeCP2_Puro

Plasmid

#167903

Purpose

PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmid

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

dCas9-KRAB-MeCP2

Use

CRISPR

Mutation

D10A, D839A, H840A, N863A

Available Since

Sept. 12, 2022

Availability

Academic Institutions and Nonprofits only
pTE1069

Plasmid

#186629

Purpose

SCB-GFP E. coli reporter plasmid. Encodes repressor ScbR and ScbAp promoter upstream of GFP.

Depositor

Eriko Takano

Article

Biarnes-Carrera et al ACS Synth Biol. 2018 Apr 20;7(4):1043-10

Inserts

scbR
scbAp promoter

Use

Synthetic Biology

Tags

6xArg

Expression

Bacterial

Promoter

BBa_J23100 and scbAp

Available Since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pAC8-Mcl1_deltaTM

Plasmid

#187118

Purpose

insect cell expression of Mcl1-deltaTM

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

MCL-1 (BCL2 Human)

Tags

Strep

Expression

Insect

Mutation

delta328-350

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pAC8-DDIT4_delta8

Plasmid

#187117

Purpose

insect cell expression of DDIT4-delta1-8

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

DDIT4 (DDIT4 Human)

Tags

Strep

Expression

Insect

Mutation

delta1-8

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-HUWE1_R4187C

Plasmid

#187141

Purpose

Entry clone

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Use

Entry clone

Mutation

R4187C

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-HUWE1_F3194S

Plasmid

#187139

Purpose

Entry clone

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Use

Entry clone

Mutation

F3194S

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-HUWE1_H669Q

Plasmid

#187137

Purpose

Entry clone

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Use

Entry clone

Mutation

H669Q

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-HUWE1_H3962D

Plasmid

#187130

Purpose

Entry clone

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Use

Entry clone

Mutation

H3962D

Available Since

Aug. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pNB101

Plasmid

#169110

Purpose

Feedback-controlled AraC circuit

Depositor

Edwin Wintermute

Article

Bongaerts et al Nat Commun. 2022 Jul 7;13(1):3905. doi:

Insert

araC (araC Escherichia coli K12 MG1655)

Use

Synthetic Biology

Expression

Bacterial

Mutation

Codon optimized for E. coli

Available Since

July 8, 2022

Availability

Academic Institutions and Nonprofits only

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pNB200

Plasmid

#169111

Purpose

TESEC backbone for target expression

Depositor

Edwin Wintermute

Article

Bongaerts et al Nat Commun. 2022 Jul 7;13(1):3905. doi:

Insert

lacZ (lacZ Escherichia coli K12 MG1655)

Use

Synthetic Biology

Expression

Bacterial

Mutation

Codon optimized for E. coli

Available Since

July 8, 2022

Availability

Academic Institutions and Nonprofits only

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pLBM0050

Plasmid

#172820

Purpose

Level 2 module containing mCHERRY-nYFP HSP21-cYFP

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

[35S-P19][35S-CTPSSU-mCHERRY-3XFLAGnYFP][35S-CTPSSU-mHSP21-cYFPX3HA][35S-SP_SV40-CFP]

Use

Synthetic Biology

Available Since

May 2, 2022

Availability

Academic Institutions and Nonprofits only

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pLBM0040

Plasmid

#172819

Purpose

Level 2 module containing HSP21-nYFP deltaPTAC5-cYFP

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

[35S-P19][35S-CTPSSU-mHSP21-3XFLAGnYFP][35S-CTPSSU-deltamPTAC5-cYFPX3HA][35S-SP_SV40-CFP]

Use

Synthetic Biology

Available Since

May 2, 2022

Availability

Academic Institutions and Nonprofits only

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pLBM0030

Plasmid

#172818

Purpose

Level 2 module containing HSP21-nYFP PTAC5-cYFP

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

[35S-P19][35S-CTPSSU-mHSP21-3XFLAGnYFP][35S-CTPSSU-mPTAC5-cYFPX3HA][35S-SP_SV40-CFP]

Use

Synthetic Biology

Available Since

May 2, 2022

Availability

Academic Institutions and Nonprofits only

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pLBM0020

Plasmid

#172817

Purpose

Level 2 module containing HSP21-nYFP HSP21-cYFP

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

[35S-P19][35S-CTPSSU-mHSP21-3XFLAGnYFP][35S-CTPSSU-mHSP21-cYFPX3HA][35S-SP_SV40-CFP]

Use

Synthetic Biology

Available Since

May 2, 2022

Availability

Academic Institutions and Nonprofits only

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pLBM0060

Plasmid

#172821

Purpose

Level 2 module containing HSP21-nYFP HSP21-cYFP OEP7-mTRQ

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

[35S-P19][35S-CTPSSU-mHSP21-3XFLAGnYFP][35S-CTPSSU-mHSP21-cYFPX3HA][OEP7_mTRQ]

Use

Synthetic Biology

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pLBM0070

Plasmid

#172822

Purpose

Level 2 module containing mCHERRY-nYFP HSP21-cYFP OEP7-mTRQ

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

[35S-P19][35S-CTPSSU-mCHERRY-3XFLAGnYFP][35S-CTPSSU-mHSP21-cYFPX3HA][OEP7_mTRQ]

Use

Synthetic Biology

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pUC19-SpRYgest_targetplasmid (KAC833)

Plasmid

#181748

Purpose

pUC19 derivative plasmid used as a substrate for SpRYgest, restriction enzyme, and TtAgo digests. Encodes an SpCas9 EMX1-site1-NGG-PAM target site between NheI and HindIII restriction sites.

Depositor

Benjamin Kleinstiver

Article

Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038

Type

Empty backbone

Use

Substrate plasmid

Available Since

Feb. 16, 2022

Availability

Academic Institutions and Nonprofits only

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