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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 14,357 results for: Cas9

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Showing: 11841 - 11860 of 14357 results

  4. AcrIIC1X

    Plasmid
    #128112
    Purpose
    AcrX expression in mammalian cells; AcrX is an engineered anti-CRISPR protein targeting S. aureus Cas9
    Depositor
    Dominik Niopek
    Publication
    Mathony et al Nat Chem Biol. 2020 Apr 13. pii: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    AcrX (AcrIIC1 N3F/D15Q/A48I)
    Use
    CRISPR and Synthetic Biology
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Dec. 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    128112-plasmid-map-sequence-id-249186

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  6. P532_POU4F2-p2A-tdTomato-p2A-Thy1.2

    Plasmid
    #239102
    Purpose
    Human donor plasmid for integration of p2A-h2b-EGFP into the 3' end of POU4F2 (aka BRN3B). This includes the donor casette and a U6 driven guide sequence to be used with Cas9.
    Depositor
    Karl Wahlin
    Publication
    Wahlin et al Front Cell Dev Biol. 2021 Nov 16;9:76472
    Insert
    POU4F2 (POU4F2 Human)
    Use
    Donor plasmid for integration into the human geno…
    Tags
    p2A-tdTomato-p2A-Thy1.2
    Expression
    Mammalian
    Promoter
    Endogenous POU4F2
    Available since
    June 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    239102-plasmid-map-sequence-id-474475

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  7. Human Brunello kinome pooled library, guides 5-8 in lentiGuide-Puro backbone

    Pooled library
    #75313
    Purpose
    Human sgRNA library in backbone lentiGuide-Puro targeting 763 kinase genes and containing 3,052 unique sgRNAs along with 100 non-targeting controls
    Depositor
    John Doench, David Root
    Publication
    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103
    Expression
    Mammalian
    Use
    CRISPR and Lentiviral
    Available since
    June 27, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by

  11. pSRG100 (germline optimized 3xGCN4 for C. elegans)

    Plasmid
    #246457
    Purpose
    Encodes germline optimized 3xGCN4 for C. elegans. Useful to make CRISPR/Cas9 repair templates for 3xGCN4 integration in a gene of interest. For SunTag live-imaging of translation or protein labelling.
    Depositor
    Suzan Ruijtenberg
    Publication
    van der Salm et al Bio Protoc. 2025 Oct 20;15(20):e5486. do
    Insert
    3xGCN4 (GLO)
    Expression
    Worm
    Available since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    246457-plasmid-map-sequence-id-496369

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  19. pYPQAT01

    Plasmid
    #223373
    Purpose
    T-DNA vector for SpCas9 mediated mutagenesis for dicot plants; NGG PAM; Cas9 was driven by AtUBQ10 and the sgRNA was driven by AtU3 promoter; Hygromycin for plants selection.
    Depositor
    Yiping Qi
    Publication
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    AtUBQ10-SpCas9-AtU3-gRNA scaffold
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223373-plasmid-map-sequence-id-454171

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Showing: 11841 - 11860 of 14357 results