We narrowed to 18,398 results for: Mut

1. 3xHA-miniTurbo-NLS_pCDNA3

   Plasmid

   #107172

   Purpose

   expresses 3xHA-tagged miniTurbo in the mammalian nucleus

   Depositor

   Alice Ting

   Article

   Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

   Insert

   miniTurbo (BirA mutant)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

   Promoter

   Available Since

   May 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDH1-CMV-FIP200-V44D/V314D/L324D/R543D/R548D-mCherry-Hygro

   Plasmid

   #210857

   Purpose

   Lentiviral expression vector encoding FIP200 mutant-mCherry

   Depositor

   James Hurley

   Article

   Chen et al bioRxiv 2023.06.01.543278

   Insert

   FIP200 (RB1CC1 Human)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mutation

   V44D/V314D/L324D/R543D/R548D

   Promoter

   Available Since

   Dec. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTECH-chPylRS(IPYE)

   Plasmid

   #99222

   Purpose

   Expression plasmid encoding chPylRS(IPYE) and pylT

   Depositor

   Dieter Söll

   Article

   Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

   Insert

   Plpp chPyl(IPYE)RS; PproK pylT
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Contains activating mutations V31I, T56P, H62Y, A…

   Promoter

   Plpp and PproK

   Available Since

   Oct. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28a-sfGFP 150TAG

   Plasmid

   #85493

   Purpose

   pET28a expressing E. coli codon optimized superfolder GFP with TAG stop codon at position 150

   Depositor

   Ryan Mehl

   Article

   Peeler et al Methods Mol Biol. 2012;794:125-34. doi:

   Insert

   superfolder GFP 150TAG
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   150 TAG (amino acid 150 mutated to stop codon)

   Promoter

   T7

   Available Since

   Jan. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. TLCV2-ApaLI*-FLAG-T2A-mTagBFP2-Puro

   Plasmid

   #211463

   Purpose

   Lentivirus backbone expressing a doxycycline-inducible mitochondrial localized ApaLI mutant (D211A, E225A and K227A)

   Depositor

   Agnel Sfeir

   Article

   Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

   Insert

   ApaLI-dead
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   D211A, E225A, K227A

   Promoter

   TRE

   Available Since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. C1(1-29)-TurboID-V5_pCDNA3

   Plasmid

   #107173

   Purpose

   expresses V5-tagged TurboID on the mammalian ER membrane

   Depositor

   Alice Ting

   Article

   Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

   Insert

   TurboID (BirA mutant)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

   Promoter

   Available Since

   May 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. miniTurbo-His6_pET21a

   Plasmid

   #107178

   Purpose

   expresses His6-tagged miniTurbo in bacteria

   Depositor

   Alice Ting

   Article

   Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

   Insert

   miniTurbo (BirA mutant)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

   Promoter

   Available Since

   May 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEB2-mKate2

   Plasmid

   #104009

   Purpose

   Plasmid encoding mKate2

   Depositor

   Philippe Cluzel

   Article

   Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

   Insert

   mKate2
   

   Use

   Low copy

   Tags

   Expression

   Bacterial

   Mutation

   Mutations relative to eqFP578 (R32G, K42R, V45A, …

   Promoter

   proC

   Available Since

   Dec. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFastBac1-6His-DHX9

   Plasmid

   #211440

   Purpose

   generation of Bacmid for insect cell expression of DHX9 N-terminally fused to 6XHis-Tag

   Depositor

   Helmut Pospiech, Frank Grosse

   Article

   Zhang et al J Biol Chem. 1997 Apr 25. 272(17):11487-

   Insert

   DHX9 (DHX9 Human)
   

   Use

   Tags

   6xHis

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   Jan. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHis-hTG2

    Plasmid

    #100719

    Purpose

    Expression of human transglutaminase 2 in E.coli

    Depositor

    Byung Il Lee

    Article

    Han et al Int J Biol Macromol. 2010 Aug 1;47(2):19

    Insert

    TGM2 (TGM2 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    silent mutation Arg580 (AGG--CGT) to enhance expr…

    Promoter

    T7

    Available Since

    Nov. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTECH-chAcK3RS(IPYE)

    Plasmid

    #104069

    Purpose

    Expression plasmid encoding chAcK3RS(IPYE) and pylT

    Depositor

    David Liu

    Article

    Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

    Insert

    Plpp chAcK3RS(IPYE); PproK pyl
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Contains activating mutations V31I, T56P, H62Y, A…

    Promoter

    Plpp and PproK

    Available Since

    Dec. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCVL Traffic Light Reporter 1.1 (Sce target) Ef1a Puro

    Plasmid

    #31482

    Depositor

    Andrew Scharenberg

    Article

    Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

    Insert

    Traffic Light Reporter 1.1 (Sce Target) EF Puro
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    mCherry has M9S and M16L mutations. This is what …

    Promoter

    Available Since

    Aug. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. pX330A_D10A-1x2

    Plasmid

    #58772

    Purpose

    Expresses Cas9 nickase and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

    Insert

    humanized S. pyogenes Cas9 (D10A) nickase
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    D10A nickase-converting mutation

    Promoter

    CBh

    Available Since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. C1(1-29)-miniTurbo-V5_pLX304

    Plasmid

    #107176

    Purpose

    expresses V5-tagged miniTurbo on the mammalian ER membrane, lentiviral vector

    Depositor

    Alice Ting

    Article

    Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

    Insert

    miniTurbo (BirA mutant)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

    Promoter

    Available Since

    May 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBI-EGFP-MnSOD

    Plasmid

    #16612

    Depositor

    Bert Vogelstein

    Article

    Hwang et al Nat Med. 2001 Oct . 7(10):1111-7.

    Insert

    MnSOD (SOD2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 7, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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16. OaAEP1_C247A

    Plasmid

    #200307

    Purpose

    Bacterial expression plasmids for production of C247A mutant of asparaginyl endopeptidase 1 from O. affinis (OaAEP1_C247A)

    Depositor

    Artur Krężel

    Article

    Antonenko et al Bioconjug Chem. 2023 Mar 15;34(4):719-27

    Insert

    Asparaginyl endopeptidase from Oldenlandia affinis
    

    Use

    Tags

    Smt3

    Expression

    Bacterial

    Mutation

    Cysteine 247 to Alanine

    Promoter

    T7

    Available Since

    June 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. mouse Clock delta 19

    Plasmid

    #177312

    Purpose

    expression of mutant mouse clock

    Depositor

    Joseph Takahashi

    Article

    King et al Cell. 1997 May 16;89(4):641-53. doi: 10.

    Insert

    mouse clock delta 19
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    deletion from original mClock gene (514-564aa)

    Promoter

    CMV

    Available Since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. lentiCas9n(D10A)-Blast

    Plasmid

    #63593

    Purpose

    Expresses human codon-optimized S. pyogenes Cas9n (D10A nickase) protein and blasticidin resistance from EFS promoter. Lentiviral backbone.

    Depositor

    Feng Zhang

    Article

    Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

    Inserts

    Cas9
    Blasticidin resistance
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    D10A, nickase mutant

    Promoter

    EFS-NS

    Available Since

    April 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEJS2700-CMV-Nme2-ABE8e-nt-E932R

    Plasmid

    #228721

    Purpose

    Expresses N-terminal ABE8e-Nme2 nCas9 (D16A) base editor with E932R mutation in mammalian cells

    Depositor

    Erik Sontheimer

    Article

    Bamidele et al bioRxiv November 07, 2024

    Insert

    N-terminal ABE8e with Nme2 nCas9 (D16A) E932R
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Nme2-Cas9 D16A E932R

    Promoter

    CMV

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pUASTattb-cAMPr Y205A, R739A

    Plasmid

    #84933

    Purpose

    cAMPr under 5x UAS regulation. pUASTTB for phiC site mediated insertion. Y205A mutation inhibits PKA-C kinase activity. R739A should increase affinity for cAMP without affecting specificity.

    Depositor

    Justin Blau

    Article

    Hackley et al Sci Signal. 2018 Mar 6;11(520). pii: 11/

    Insert

    cAMPr Y205A R739A
    

    Use

    Tags

    Expression

    Insect

    Mutation

    PKA-C Y205A, PKA-R R739A

    Promoter

    5X UAS

    Available Since

    March 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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