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pMX1433

Plasmid

#127857

Purpose

Expresses EGFP-deltaN-term-MCAK in mammalian cells

Depositor

Linda Wordeman

Article

Domnitz et al J Cell Biol. 2012 Apr 16;197(2):231-7. d

Insert

deltaN-term-MCAK

Tags

EGFP

Expression

Mammalian

Mutation

deletion of first 19 codons of MCAK

Promoter

CMV

Available Since

July 5, 2019

Availability

Academic Institutions and Nonprofits only

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Keratin 18 S33A

Plasmid

#18061

Depositor

M Bishr Omary

Article

Ku et al J Cell Biol. 1994 Oct . 127(1):161-71.

Insert

Keratin 18 (KRT18 Human)

Expression

Mammalian

Mutation

S33A

Available Since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only
HA Raptor mutant 2

Plasmid

#8471

Depositor

David Sabatini

Article

Kim et al Cell 2002 Jul 26;110(2):163-75.

Insert

Raptor (RPTOR Human)

Tags

ha

Expression

Mammalian

Mutation

261DLF263-AAA (D261A, L262A, F263A)

Available Since

May 11, 2005

Availability

Academic Institutions and Nonprofits only
MLM3367

Plasmid

#43884

Purpose

Activation Domain: VP64; RVD of C-terminal 0.5 TALE repeat: NI (binds A)

Depositor

Keith Joung

Article

Maeder et al Nat Methods. 2013 Feb 10. doi: 10.1038/n

Insert

TALE-activator VP64 Backbone

Use

Tale transcriptional activator expression vector

Promoter

EF1alpha

Available Since

March 8, 2013

Availability

Academic Institutions and Nonprofits only

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RCAS (A) cTbx3 del2 (CT#645)

Plasmid

#13921

Depositor

Cliff Tabin

Article

Logan et al Development. 1998 Aug . 125(15):2825-35.

Insert

Tbx3 del (TBX3 Chicken)

Use

Retroviral; Avian expression

Mutation

Truncated Tbx3. Putative dominant negative.

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
WT-short-UBASH3A-V5-6xHis

Plasmid

#192102

Purpose

Mammalian expression of human wild-type short-form UBASH3A with V5-6xHis tag

Depositor

Patrick Concannon

Article

Ge et al J Immunol. 2019 Dec 1;203(11):2827-2836.

Insert

UBASH3A (UBASH3A Human)

Tags

V5, 6xHis

Expression

Mammalian

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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V5-W279A-short-UBASH3A

Plasmid

#192086

Purpose

Mammalian expression of human W279A short-form UBASH3A with V5 tag

Depositor

Patrick Concannon

Article

Ge et al J Immunol. 2019 Dec 1;203(11):2827-2836.

Insert

UBASH3A (UBASH3A Human)

Tags

V5

Expression

Mammalian

Mutation

W279A

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pDD397

Plasmid

#91822

Purpose

AID FP-slot donor for the SapTrap cloning system

Depositor

Bob Goldstein

Article

Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

Insert

Auxin-inducible degron (AID)

Use

CRISPR

Expression

Worm

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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pCR8/GW:PtU6

Plasmid

#104895

Purpose

Phaeodactylum U6 promoter

Depositor

Thomas Mock

Article

Graff van Creveld et al Front Microbiol. 2021 Sep 8;12:688199. d

Insert

Phaeodactylum U6 promoter

Available Since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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pMDR1-136

Plasmid

#69482

Purpose

Deletion Construct

Depositor

Kathleen Scotto

Article

Jin et al Mol Cell Biol. 1998 Jul;18(7):4377-84.

Insert

MDR1 promoter (ABCB1 Human)

Use

Luciferase

Expression

Mammalian

Mutation

Insert includes MDR1 promoter sequence from -136 …

Available Since

Nov. 16, 2015

Availability

Academic Institutions and Nonprofits only
pMiniWhiteA2

Plasmid

#165873

Purpose

Basic transgenesis Mini-white physical eye marker cassette in Alpha2, lacks attB site

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Eye Marker Cassette

Use

Synthetic Biology

Available Since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEX-SaCas9-U6-sgKcnab2

Plasmid

#192795

Purpose

Encodes SaCas9 and an sgRNA targeting mouse Kcnab2

Depositor

Marta Soden

Article

Yee et al J Neurophysiol. 2022 Jul 1;128(1):62-72.

Insert

sgKcnab2

Use

AAV and CRISPR

Promoter

U6

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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RASL11A

Plasmid

#55685

Purpose

expression of mouse RASL11A

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

RASL11A (Rasl11a Mouse)

Expression

Bacterial

Promoter

lac

Available Since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only
pAW79.pUC19.noYY1

Plasmid

#104377

Purpose

No YY1 binding motifs as control for in vitro ligation

Depositor

Richard Young

Article

Weintraub et al Cell. 2017 Dec 14;171(7):1573-1588.e28.

Insert

No YY1 binding motifs

Available Since

Feb. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pAW49.pUC19.YY1

Plasmid

#104376

Purpose

Contains YY1 binding sites for in vitro ligation

Depositor

Richard Young

Article

Weintraub et al Cell. 2017 Dec 14;171(7):1573-1588.e28.

Insert

YY1 binding motifs

Available Since

Feb. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pTXG-hTDG

Plasmid

#52281

Purpose

Bacterial expression of human TDG C-terminally fused to GyrA intein and GST

Depositor

Primo Schaer

Article

Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

Insert

hTDG (TDG Human)

Tags

GyrA-intein-GST

Expression

Bacterial

Promoter

T7

Available Since

July 10, 2014

Availability

Academic Institutions and Nonprofits only

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pBS NFATC1

Plasmid

#19203

Depositor

Cliff Tabin

Article

Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

Insert

NFATC1 (NFATC1 Chicken)

Expression

Bacterial

Available Since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only
bTub-GFP.766A

Plasmid

#112695

Purpose

expressed GFP in testes, under bTub promoter

Depositor

Omar Akbari

Article

Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

Insert

bTub-GFP-SV40

Expression

Insect

Promoter

bTub

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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FH95pUGW (B3)

Plasmid

#74012

Purpose

lentiviral expression of EGFP and Psd95 shRNA

Depositor

Robert Malenka, Oliver Schluter, Weifeng Xu

Article

Schluter et al Neuron. 2006 Jul 6;51(1):99-111.

Insert

Psd95

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

H1

Available Since

Jan. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pBbS8k-RFP_R46Q

Plasmid

#109414

Purpose

RepA Mutant

Depositor

Jay Keasling

Article

Thompson et al Sci Rep. 2018 Jan 25;8(1):1590. doi: 10.

Insert

repA

Tags

RFP

Expression

Bacterial

Mutation

R46Q repA mutation to increase copy number

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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