We narrowed to 12,986 results for: NUC

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pcDNA3-myc NES- mCE(K294A)

Plasmid

#82464

Purpose

Expresses myc tag and catalytic inactive form of mRNA capping enzyme with N terminal Nuclear export signal (NES)

Depositor

Daniel Schoenberg

Article

Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d

Insert

NES mRNA capping enzyme (Rngtt Mouse, HIV)

Tags

myc

Expression

Mammalian

Mutation

K294A

Promoter

CMV

Available Since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

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mPapaya1-CENPB-N-22

Plasmid

#56351

Purpose

Localization: Nucleus/Centromeres, Excitation: 530, Emission: 541

Depositor

Michael Davidson

Article

Hoi et al Chem Biol. 2013 Oct 24;20(10):1296-304.

Insert

CENPB (CENPB Human)

Tags

mPapaya1

Expression

Mammalian

Mutation

aa 1-169 of CENPB (DNA Binding Domain)

Promoter

CMV

Available Since

Dec. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pmTol-InsUAS-6MTnlstdTomato2A-Dusp6DM-CC

Plasmid

#67687

Purpose

UAS-driven 6xMyc nuclear localized tdTomato-2A-activated Dusp6 in minTol backbone; also with cry:cfp cassette

Depositor

Nathan Lawson

Article

Shin et al Development. 2016 Oct 15;143(20):3796-38

Insert

dusp6 (dusp6 Zebrafish)

Tags

6xmyc-NLS-tdTomato-2A

Mutation

S159A, S196A

Available Since

Oct. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pCEP-WAF1-ES

Plasmid

#16449

Depositor

Bert Vogelstein

Article

el-Deiry et al Cell. 1993 Nov 19. 75(4):817-25.

Insert

WAF1 stop codon mut (CDKN1A Human)

Expression

Mammalian

Mutation

stop codon at amino acid 49 instead of tryptophan…

Available Since

April 7, 2008

Availability

Academic Institutions and Nonprofits only
KHBD00681

Plasmid

#39726

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG14513 (yemalpha Fly)

Use

Gateway donr vector

Promoter

none

Available Since

Sept. 17, 2012

Availability

Academic Institutions and Nonprofits only
mTFP1-CENPB-N-22

Plasmid

#55470

Purpose

Localization: Nucleus/Centromeres, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

CENPB (CENPB Human)

Tags

mTFP1

Expression

Mammalian

Mutation

aa 1-169 of CENPB (DNA Binding Domain)

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pHyg-AID(1-114)

Plasmid

#99513

Purpose

Truncated C-terminal AID degron cassette with HygR marker for S. cerevisiae

Depositor

Helle Ulrich

Article

Morawska et al Yeast. 2013 Sep;30(9):341-51. doi: 10.10

Insert

IAA17(1-114) (AXR3 Mustard Weed)

Use

Pcr template for yeast tagging cassette

Mutation

internal inversion of nucleotides 265-312 of the …

Available Since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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mTFP1-H3.3-N-14

Plasmid

#55490

Purpose

Localization: Nucleus/Histones, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

H3.3 (H3f3b Mouse)

Tags

mTFP1

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
KHBD00294

Plasmid

#34420

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG9310 (Hnf4 Fly)

Use

Gateway donor vector

Available Since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
pUAS-Tum

Plasmid

#37304

Depositor

Norbert Perrimon

Article

Harrison et al EMBO J. 1995 Jun 15;14(12):2857-65.

Insert

hopTum-1 (hop Fly)

Expression

Insect

Mutation

Tul-l mutation is a G to A transversion at nucleo…

Promoter

Hsp70

Available Since

July 23, 2012

Availability

Academic Institutions and Nonprofits only
AAV-eGFP-KASH

Plasmid

#248781

Purpose

AAV construct expressing eGFP-KASH enabling FACS-sorting of extracted nuclei

Depositor

David Liu

Article

Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

Insert

eGFP-KASH

Promoter

Cbh

Available Since

Jan. 5, 2026

Availability

Academic Institutions and Nonprofits only

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pET-hisMBP-rRNaseI

Plasmid

#216673

Purpose

Expresses hisMBP-tagged rat oxidation-resistant RNase inhibitor in E. coli hosts

Depositor

Po-Min Chiang

Article

Hsueh et al PLoS Biol. 2025 Dec 15;23(12):e3003537.

Insert

ribonuclease inhibitor (Rnh1 Rat)

Tags

his-tagged MBP

Expression

Bacterial

Promoter

T7

Available Since

Nov. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pWidom601.CDKN2Bp.601

Plasmid

#247019

Purpose

Two Widom601 sequences, with a short linker with sequence from the CDKN2B locus between them. For large scale PCR amplification of DNA for nucleosome refolding.

Depositor

Chen Davidovich

Article

Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

Insert

Widom601 sequences and CDKN2B locus

Use

Synthetic Biology

Available Since

Nov. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pKMW124_dCas9

Plasmid

#244821

Purpose

Mammalian expression of catalytically inactive Cas9 (dCas9)

Depositor

Jennifer Doudna

Article

Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

Insert

dCas9 (WT)

Use

CRISPR

Tags

3xFLAG-SV40 NLS and Nucleoplasmin NLS

Expression

Mammalian

Mutation

D10A/H840A

Promoter

CAG, U6

Available Since

Sept. 17, 2025

Availability

Academic Institutions and Nonprofits only

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Split PS Intein gal4-vp16

Plasmid

#242030

Purpose

Blue-light-controlled release of gal4-vp16 transactivator from the cytoplasm to the nucleus. Split version.

Depositor

Vladislav Verkhusha

Article

Baloban et al Nat Commun. 2025 Sep 11;16(1):8263. doi:

Insert

Split PS Intein gal4-vp16

Expression

Mammalian

Mutation

pNL3.2.NF-KB-RE[NlucP/NF-KB-RE/Hygro]

Promoter

CMV

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiRNACRISPR_011-TetO-NLS-inactive-RfxCas13d-NLS-WPRE-EFS-rtTA3-2A-Blast

Plasmid

#228557

Purpose

Expresses inactive RfxCas13d in mammalian cells for binding of target RNAs in combination with compatible crRNAs. Localized to the nucleus

Depositor

Neville Sanjana

Article

Hart et al Nat Biotechnol. 2025 Feb 11. doi: 10.103

Insert

dRfxCas13d

Use

Lentiviral

Tags

NLS-HA

Mutation

R239A/H244A/R858A/H863A

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV_CaMKII-RFP-p2A-DAAO-NES

Plasmid

#238918

Purpose

Mammalian expression of DAAO with a nuclear export signal and RFP as a reporter in excitatory neurons

Depositor

Vsevolod Belousov

Article

Kalinichenko et al Redox Biol. 2023 Apr;60:102604. doi: 10.

Insert

RFP-p2A-DAAO-NES

Use

AAV

Expression

Mammalian

Promoter

CaMKIIalfa

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV_CMV-RFP-p2A-DAAO-NES

Plasmid

#238920

Purpose

Expression of DAAO with a nuclear export signal and RFP as a reporter in mammalian cells

Depositor

Vsevolod Belousov

Article

Kalinichenko et al Redox Biol. 2023 Apr;60:102604. doi: 10.

Insert

RFP-p2A-DAAO-NES

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pQdCas12a.luxR(mut)-sggfp(B)

Plasmid

#236041

Purpose

The plasmid pQdCas12a.luxR(mut)-sggfp(B) expresses the dCas12a endonuclease and the sgRNA (design B) targeting the gfp gene. Additionally, it contains a mutation in the luxR gene.

Depositor

Radhakrishnan Mahadevan

Article

Golla et al ACS Synth Biol. 2025 Oct 17;14(10):3890-

Insert

quorum sensing cassette luxI, mutated luxR, and the LuxR-dependent promoter pLuxI , dCas12a and sgRNA design (B) of gfp gene

Use

CRISPR

Promoter

quorum sensing promoter

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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pQdCas12a.luxR(mut)-sggfp(A)

Plasmid

#236039

Purpose

The plasmid pQdCas12a.luxR(mut)-sggfp(A) expresses the dCas12a endonuclease and the sgRNA (design A) targeting the gfp gene. Additionally, it contains a mutation in the luxR gene.

Depositor

Radhakrishnan Mahadevan

Article

Golla et al ACS Synth Biol. 2025 Oct 17;14(10):3890-

Insert

quorum sensing cassette luxI, mutated luxR, and the LuxR-dependent promoter pLuxI , dCas12a and sgRNA design (B) of gfp gene

Use

CRISPR

Promoter

quorum sensing promoter

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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