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pMT-Aurora B-GFP

Plasmid

#24285

Depositor

Ron Vale

Article

Vale et al J Cell Biol. 2009 Sep 7. 186(5):727-38.

Insert

Aurora B Kinase (aurB Fly)

Tags

GFP

Expression

Insect

Available Since

May 10, 2010

Availability

Academic Institutions and Nonprofits only
pHRSin_hTCRαγε

Plasmid

#187352

Purpose

Lentiviral expression of GPa3b17 hTCR alpha, and truncated hCD3 (gamma, and epsilon)

Depositor

Simon Davis

Article

Susac et al Cell. 2022 Aug 18;185(17):3201-3213.e19.

Insert

GPa3b17 hTCR alpha, and truncated hCD3 (gamma, and epsilon)

Use

Lentiviral

Promoter

SFFV

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pHRSin_hTCRβε

Plasmid

#187354

Purpose

Lentiviral expression of GPa3b17 hTCR beta, and truncated hCD3 epsilon

Depositor

Simon Davis

Article

Susac et al Cell. 2022 Aug 18;185(17):3201-3213.e19.

Insert

GPa3b17 hTCR beta, and truncated hCD3 epsilon

Use

Lentiviral

Promoter

SFFV

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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HA-DN-14-3-3 zeta (R56A/R60A)

Plasmid

#116889

Purpose

Expresses HA-tagged human dominant-negative 14-3-3 zeta (R56A/R60A) in mammalian cells

Depositor

FENG-QIAN Li, Ken-Ichi Takemaru

Article

Li et al J Cell Biol. 2008 Jun 30;181(7):1141-54.

Insert

DN-14-3-3 zeta (R56A/R60A) (YWHAZ Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pFH2.107_PB-EPN24-MCP_p6-T2A-eGFP-PGK-Blast

Plasmid

#205552

Purpose

Express EPN24-MCP-p6-T2A-eGFP and blasticidin resistance marker

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

EPN24-MCP-p6

Use

Piggybac

Expression

Mammalian

Mutation

WT

Promoter

EF1

Available Since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pSmart-EF1a-Nla-cas11

Plasmid

#178882

Purpose

Expression of human codon-optimized NlaCas11 with N terminal NLS and HA tag

Depositor

Yan Zhang

Article

Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

Insert

EF1a-NlaCas11c-bGH polyA

Tags

HA and NLS

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 18, 2022

Availability

Academic Institutions and Nonprofits only

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V2-MESA-35F-Tev

Plasmid

#84503

Purpose

MESA protease chain with V2-MESA ectodomain, 35 extracellular linkers, a flag tag, and Tev protease

Depositor

Joshua Leonard

Article

Schwarz et al Nat Chem Biol. 2016 Dec 12. doi: 10.1038

Insert

V2-MESA-35F-Tev

Use

AAV

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pFH2.104_PB-EPN24-T2A-eGFP-PGK-Blast

Plasmid

#205549

Purpose

Express EPN24-T2A-eGFP and blasticidin resistance marker

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

EPN24

Use

Piggybac

Expression

Mammalian

Mutation

WT

Promoter

EF1

Available Since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

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V1-MESA-45F-Tev

Plasmid

#84501

Purpose

MESA protease chain with V1-MESA ectodomain, 45 extracellular linkers, a flag tag, and Tev protease

Depositor

Joshua Leonard

Article

Schwarz et al Nat Chem Biol. 2016 Dec 12. doi: 10.1038

Insert

V1-MESA-45F-Tev

Use

AAV

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 11, 2017

Availability

Academic Institutions and Nonprofits only

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Lenti-sgRNA(MS2)-puro-barcode

Plasmid

#89493

Purpose

Cloning backbone for sgRNA, also has a barcode that allows detection from scRNA-seq

Depositor

Gary Hon

Article

Xie et al Mol Cell. 2017 Apr 20;66(2):285-299.e5.

Insert

sgRNA (with MS2 loop)

Use

Lentiviral

Promoter

U6

Available Since

June 30, 2017

Availability

Academic Institutions and Nonprofits only

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