Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 12,321 results for: LEA

Clear keywords
Showing: 11961 - 11980 of 12321 results

  1. PWPXLD-EGFR-P667A

    Plasmid
    #133750
    Purpose
    EGFR with mutation at proline 667 converted to alanine and tagged with HA cloned into PWPXLD plasmid
    Depositor
    Chay Kuo
    Publication
    Abdi et al Cell Rep. 2019 Aug 20;28(8):2012-2022.e4
    Insert
    Mutant P667A EGFR-HA (EGFR Human)
    Use
    Lentiviral
    Tags
    HA tag
    Expression
    Mammalian
    Mutation
    proline 667 converted to alanine (Please see depo…
    Promoter
    EF1-alpha
    Available since
    May 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    133750-plasmid-map-sequence-id-264885

    Enlarge

  2. pcDNA-GFP-EBA175-Flag(E1418K/P1424Q/Y1426S)

    Plasmid
    #155006
    Purpose
    Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 E1418K/P1424Q/Y1426S variant (residues 1284-1462) from pcDNA3.1
    Depositor
    Sinisa Urban
    Publication
    Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-
    Insert
    PfEBA175
    Tags
    Flag, GFP, and signal peptide (residues 1 to 32) …
    Expression
    Mammalian
    Mutation
    E1418K/P1424Q/Y1426S; insert codes for EBA175 res…
    Promoter
    CMV
    Available since
    Nov. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    155006-plasmid-map-sequence-id-309986

    Enlarge

  3. pcDNA-GFP-EBA175-Flag(M1423R/P1424V/Y1425W/Y1426H)

    Plasmid
    #155313
    Purpose
    Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 M1423R/P1424V/Y1425W/Y1426H variant (residues 1284-1462) from pcDNA3.1
    Depositor
    Sinisa Urban
    Publication
    Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-
    Insert
    PfEBA175
    Tags
    Flag, GFP, and signal peptide (residues 1 to 32) …
    Expression
    Mammalian
    Mutation
    M1423R/P1424V/Y1425W/Y1426H; insert codes for EBA…
    Promoter
    CMV
    Available since
    Nov. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    155313-plasmid-map-sequence-id-310080

    Enlarge

  4. pcDNA-GFP-EBA175-Flag(M1423R/P1424V/Y1426H)

    Plasmid
    #155312
    Purpose
    Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 M1423R/P1424V/Y1426H variant (residues 1284-1462) from pcDNA3.1
    Depositor
    Sinisa Urban
    Publication
    Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-
    Insert
    PfEBA175
    Tags
    Flag, GFP, and signal peptide (residues 1 to 32) …
    Expression
    Mammalian
    Mutation
    M1423R/P1424V/Y1426H; insert codes for EBA175 res…
    Promoter
    CMV
    Available since
    Nov. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    155312-plasmid-map-sequence-id-309984

    Enlarge

  5. pcDNA-GFP-EBA175-Flag(A1419R/P1424Q/Y1426S)

    Plasmid
    #155007
    Purpose
    Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 A1419R/P1424Q/Y1426S variant (residues 1284-1462) from pcDNA3.1
    Depositor
    Sinisa Urban
    Publication
    Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-
    Insert
    PfEBA175
    Tags
    Flag, GFP, and signal peptide (residues 1 to 32) …
    Expression
    Mammalian
    Mutation
    A1419R/P1424Q/Y1426S; insert codes for EBA175 res…
    Promoter
    CMV
    Available since
    Nov. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    155007-plasmid-map-sequence-id-309775

    Enlarge

  6. pcDNA-GFP-EBA175-Flag(S1421R/P1424Q/Y1426S)

    Plasmid
    #155008
    Purpose
    Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 S1421R/P1424Q/Y1426S variant (residues 1284-1462) from pcDNA3.1
    Depositor
    Sinisa Urban
    Publication
    Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-
    Insert
    PfEBA175
    Tags
    Flag, GFP, and signal peptide (residues 1 to 32) …
    Expression
    Mammalian
    Mutation
    S1421R/P1424Q/Y1426S; insert codes for EBA175 res…
    Promoter
    CMV
    Available since
    Nov. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    155008-plasmid-map-sequence-id-309834

    Enlarge

  7. pcDNA-GFP-EBA175-Flag(E1418K/A1419R/P1424Q/Y1426S)

    Plasmid
    #155009
    Purpose
    Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 E1418K/A1419R/P1424Q/Y1426S variant (residues 1284-1462) from pcDNA3.1
    Depositor
    Sinisa Urban
    Publication
    Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-
    Insert
    PfEBA175
    Tags
    Flag, GFP, and signal peptide (residues 1 to 32) …
    Expression
    Mammalian
    Mutation
    E1418K/A1419R/P1424Q/Y1426S; insert codes for EBA…
    Promoter
    CMV
    Available since
    Nov. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    155009-plasmid-map-sequence-id-309962

    Enlarge

  8. pcDNA-GFP-EBA175-Flag(P1424I/Y1425I/Y1426I)

    Plasmid
    #154977
    Purpose
    Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 P1424A/Y1425I/Y1426I variant (residues 1284-1462) from pcDNA3.1
    Depositor
    Sinisa Urban
    Publication
    Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-
    Insert
    PfEBA175
    Tags
    Flag, GFP, and signal peptide (residues 1 to 32) …
    Expression
    Mammalian
    Mutation
    P1424/Y1425I/Y1426I; insert codes for EBA175 resi…
    Promoter
    CMV
    Available since
    Nov. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    154977-plasmid-map-sequence-id-309851

    Enlarge

  9. vPyACR_2164382

    Plasmid
    #153029
    Purpose
    Anion-conducting channelrhodopsin of viral origin. Codon-optimized for mammalian expression (human/mouse).
    Depositor
    Peter Hegemann
    Publication
    Rozenberg et al Curr Biol. 2020 Dec 21;30(24):4910-4920.
    Insert
    vPyACR_2164382
    Tags
    eYFP
    Expression
    Mammalian
    Mutation
    vPyACR_2164382 was C-terminally truncated to incl…
    Promoter
    CMV (+ enhancer)
    Available since
    Oct. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    153029-plasmid-map-sequence-id-300511

    Enlarge

  10. vPyACR_21821

    Plasmid
    #153030
    Purpose
    Anion-conducting channelrhodopsin of viral origin. Codon-optimized for mammalian expression (human/mouse).
    Depositor
    Peter Hegemann
    Publication
    Rozenberg et al Curr Biol. 2020 Dec 21;30(24):4910-4920.
    Insert
    vPyACR_21821
    Tags
    eYFP
    Expression
    Mammalian
    Mutation
    vPyACR_21821 was C-terminally truncated to includ…
    Promoter
    CMV (+ enhancer)
    Available since
    Oct. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    153030-plasmid-map-sequence-id-300513

    Enlarge

  11. pGEX-HA-GlpG(D243G/L244G/F245G/M247G/S248G/M249G/A250G)

    Plasmid
    #155136
    Purpose
    Expresses N-terminally GFP- and HA-tagged E. coli GlpG D243G/L244G/F245G/M247G/S248G/M249G/A250G variant from pGEX 4P-1
    Depositor
    Sinisa Urban
    Publication
    Cho et al Nat Struct Mol Biol. 2019 Oct;26(10):910
    Insert
    GlpG (glpG Escherichia coli)
    Tags
    GST and HA
    Expression
    Bacterial
    Mutation
    D243G/L244G/F245G/M247G/S248G/M249G/A250G
    Promoter
    tac
    Available since
    Sept. 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    155136-plasmid-map-sequence-id-310495

    Enlarge

  12. pcDNA3-Venus-cpVenus-FLARE-EKAR-EV(T/A)

    Plasmid
    #123340
    Purpose
    Negative control mutant for Venus-cpVenus-FLARE-EKAR-EV biosensor.
    Depositor
    Jin Zhang
    Publication
    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10
    Insert
    Venus-cpVenus-FLARE-EKAR-EV(T/A)
    Tags
    6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…
    Expression
    Mammalian
    Mutation
    Target Thr residue in substrate domain mutated to…
    Promoter
    CMV
    Available since
    Aug. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    123340-plasmid-map-sequence-id-307135

    Enlarge

  13. pGBW-m4046565

    Plasmid
    #149097
    Purpose
    Yeast Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    Tags
    Cleavable thrombin;StrepII
    Expression
    Yeast
    Mutation
    Saccharomyces cerevisiae recode 1
    Promoter
    pTEF1
    Available since
    May 14, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149097-plasmid-map-sequence-id-299947

    Enlarge

  14. pRevA-TEV-His12

    Plasmid
    #137034
    Purpose
    expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi RevA with C-terminal TEV-protease-cleavable His12
    Depositor
    Debra Hansen
    Publication
    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10
    Insert
    AAF07416.1 (revA Borrelia burgdorferi B31)
    Tags
    His12 (TEV protease cleavable)
    Expression
    Bacterial
    Mutation
    wild type, full-length protein, contains signal p…
    Promoter
    T7
    Available since
    March 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    137034-plasmid-map-sequence-id-275692

    Enlarge

  15. pP13-TEV-His12

    Plasmid
    #137035
    Purpose
    expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi P13 with C-terminal TEV-protease-cleavable His12
    Depositor
    Debra Hansen
    Publication
    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10
    Insert
    AAC66426.1 (BB_0034 Borrelia burgdorferi B31)
    Tags
    His12 (TEV protease cleavable)
    Expression
    Bacterial
    Mutation
    wild type, full-length protein, contains signal p…
    Promoter
    T7
    Available since
    March 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    137035-plasmid-map-sequence-id-275690

    Enlarge

  17. pCEP4-191084-QES.i06.c07

    Plasmid
    #123239
    Purpose
    Mammalian expression plasmid for Env from the 191084 B7-19 HIV-1 isolate; QES mutant with enhanced binding to PG16
    Depositor
    Erik Procko
    Publication
    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.
    Insert
    HIV-1 (191084 B7-19) Env
    Tags
    CD5 leader peptide
    Expression
    Mammalian
    Mutation
    Codon-optimized synthetic gene; Mutations P124D, …
    Promoter
    CMV
    Available since
    May 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    123239-plasmid-map-sequence-id-235191

    Enlarge

  18. pCEP4-001428(753)-VC

    Plasmid
    #123256
    Purpose
    Mammalian expression plasmid for Env from the 001428 HIV-1 isolate; C-terminal truncation fused to the C-terminal half of split fluorescent Venus (VC)
    Depositor
    Erik Procko
    Publication
    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.
    Insert
    HIV-1 (001428) Env
    Tags
    CD5 leader peptide and VC (Venus residues D155–K2…
    Expression
    Mammalian
    Mutation
    Codon-optimized synthetic gene; C-terminal Env re…
    Promoter
    CMV
    Available since
    April 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    123256-plasmid-map-sequence-id-235217

    Enlarge

  19. pCEP4-ZM197M-QES.c10-754*

    Plasmid
    #123222
    Purpose
    Mammalian expression plasmid for Env from the ZM197M HIV-1 isolate; C-terminal truncation; QES mutant with enhanced binding to PG16
    Depositor
    Erik Procko
    Publication
    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.
    Insert
    HIV-1 (ZM197M) Env
    Tags
    CD5 leader peptide
    Expression
    Mammalian
    Mutation
    Codon-optimized synthetic gene; Premature stop co…
    Promoter
    CMV
    Available since
    April 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    123222-plasmid-map-sequence-id-235165

    Enlarge

  20. pCEP4-Q769-QES.i03.c04

    Plasmid
    #123229
    Purpose
    Mammalian expression plasmid for Env from the Q769.d22 HIV-1 isolate; QES mutant with enhanced binding to PG16
    Depositor
    Erik Procko
    Publication
    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.
    Insert
    HIV-1 (Q769.d22) Env
    Tags
    CD5 leader peptide
    Expression
    Mammalian
    Mutation
    Codon-optimized synthetic gene; Mutations A200E, …
    Promoter
    CMV
    Available since
    April 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    123229-plasmid-map-sequence-id-235175

    Enlarge

Showing: 11961 - 11980 of 12321 results