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pMRE-Tn7-156

Plasmid

#118570

Purpose

miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Publication

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mCardinal

Use

Minitn7 delivery vector

Expression

Bacterial

Available since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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Human USP30 (1-517, C77A, MG-38-16)

Plasmid

#110749

Purpose

Transient transfection of human USP30 C77A

Depositor

David Komander

Publication

Gersch et al Nat Struct Mol Biol. 2017 Sep 25. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

USP30 (USP30 Human)

Expression

Mammalian

Mutation

C77A

Promoter

CMV

Available since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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pCZGY2851

Plasmid

#87761

Purpose

Punc-17b-PH::miniSOG(Q103L), expression of enhanced mutant version of membrane targeted PH-miniSOG (Q103L) in cholinergic motor neurons.

Depositor

Andrew Chisholm

Publication

Xu et al Sci Rep. 2016 Feb 10;6:21271. doi: 10.10

Insert

Punc-17b-PH-miniSOG(Q103L)

Tags

PH

Expression

Worm

Promoter

Punc-17b

Available since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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pET28a-MSP1D1deltaH4/2

Plasmid

#71712

Purpose

Membrane scaffold protein (MSP), half of helix 4 truncated

Depositor

Gerhard Wagner

Publication

Hagn et al J Am Chem Soc. 2013 Feb 6;135(5):1919-25

Insert

Apolipoprotein A1 (APOA1 Human)

Tags

6xHis and TEV site

Expression

Bacterial

Available since

Dec. 15, 2015

Availability

Academic Institutions and Nonprofits only

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GSK3beta (K85A)-pcw107-V5

Plasmid

#64614

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Publication

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

GSK3B (GSK3B Human)

Use

Lentiviral

Tags

V5

Mutation

K85A

Promoter

PGK

Available since

May 12, 2015

Availability

Academic Institutions and Nonprofits only
pTR-UF11 (AAV.A11.T)

Viral prep

#157970-AAV.A11.T

Purpose

Ready-to-use AAV44.9 in PBS particles produced from pTR-UF11 (#157970). In addition to the viral particles, you will also receive purified pTR-UF11 plasmid DNA. 'Humanized' GFP driven by Chimeric CMV/Chicken Beta Actin promoter

Depositor

Sergei Zolotukhin

Publication

Burger et al Mol Ther. 2004 Aug;10(2):302-17. doi: 10

Promoter

chimeric CMV/Chicken Beta actin (CBA)

Tags

GFP

Available since

Dec. 21, 2020

Availability

Academic Institutions and Nonprofits only
V37 pDNR MCS SA - Donor

Plasmid

#11690

Depositor

Tony Pawson

Publication

Colwill et al BMC Biotechnol. 2006 Mar 6;6:13. doi: 10

Type

Empty backbone

Use

Cre/Lox; Donor vector

Available since

July 19, 2006

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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pSH124-ssr

Plasmid

#198025

Purpose

Expresses the gene codying for the Ssr recombinase from Pseudomonas putida DOT-T1E

Depositor

Susanne Häußler

Publication

Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

Insert

T0 terminator - Ap/Cb resistance cassette

Expression

Bacterial

Promoter

ApR promoter

Available since

Oct. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pLX302 GDF11-V5 puro

Plasmid

#83097

Purpose

Lentiviral vector for constitutive expression of human GDF11 with C-terminal V5 tag

Depositor

Kevin Janes

Publication

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

GDF11 (GDF11 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pKK-TEV-3XFLAG

Plasmid

#105784

Purpose

Expression of your protein of interest in fusion with 3xFLAG at the C-terminus. The tag is cleavable by TEV protease.

Depositor

Andrzej Dziembowski

Publication

Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

Type

Empty backbone

Use

Flp-in competent

Tags

TEV-3XFLAG

Expression

Mammalian

Available since

Feb. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pKK-MBP-TEV

Plasmid

#105771

Purpose

Expression of your protein of interest in fusion with MBP at the N-terminus (cleavable by TEV). The maltose binding protein tag allows convenient purification of proteins (PMID: 22442034, 19935684).

Depositor

Andrzej Dziembowski

Publication

Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

Type

Empty backbone

Use

Flp-in competent

Tags

MBP-TEV

Expression

Mammalian

Available since

Feb. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pCrysβ:ECFP-LexOP:mCherry-KrasG12V

Plasmid

#89888

Purpose

Human KRasG12V fused to mCherry downstream of LexOP, placed within a non-autonomous maize dissociation (Ds) element for integration in zebrafish. Contains pCrysB-ECFP for screening.

Depositor

Tatjana Sauka-Spengler

Publication

Kenyon et al Dis Model Mech. 2018 Apr 6;11(4). pii: d

Insert

mCherry-KRasG12V

Use

Unspecified

Tags

mCherry

Available since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pLX-V5-TRAF2-puro

Plasmid

#44111

Depositor

William Hahn

Publication

Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

Insert

TRAF2 (TRAF2 Human)

Use

Lentiviral

Tags

V5

Available since

March 26, 2013

Availability

Academic Institutions and Nonprofits only
AP-SEMA3A (chick)

Plasmid

#29448

Depositor

Jonathan Raper

Publication

Feiner et al Neuron. 1997 Sep . 19(3):539-45.

Insert

AP-SEMA3A (chick) (SEMA3A Chicken)

Tags

AP tagged

Expression

Mammalian

Available since

July 1, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
pDONR223-MPP2

Plasmid

#23673

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MPP2 (MPP2 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pLG019 (AVAST type 3)

Plasmid

#157897

Purpose

Expresses the AVAST type 3 defense system

Depositor

Feng Zhang

Publication

Gao et al Science. 2020 Aug 28;369(6507):1077-1084

Insert

AVAST type 3 (STAND ATPase with N-terminal DUF4297)

Expression

Bacterial

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pSEVA1213S

Plasmid

#122095

Purpose

constitutive expression of I-SceI for genome engineering in Pseudomonas

Depositor

Pablo Ivan Nikel

Publication

Wirth et al Microb Biotechnol. 2019 Mar 12. doi: 10.

Insert

I-SceI restriction endonuclease

Available since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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UbcH5A

Plasmid

#61081

Purpose

express a human E2

Depositor

Cynthia Wolberger

Publication

Wiener et al Nat Struct Mol Biol. 2013 Sep;20(9):1033

Insert

UbcH5A (UBE2D1 Human)

Expression

Bacterial

Promoter

T7

Available since

Jan. 29, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pSP72 GSK3beta

Plasmid

#60125

Purpose

for transcription of GSK3beta

Depositor

Andrew McMahon

Insert

Glycogen synthase kinase 3 beta (Gsk3b Mouse)

Use

Cloning

Available since

Jan. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pLKO-shCIAP2-A

Plasmid

#44130

Depositor

William Hahn

Publication

Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

Insert

CIAP2

Use

Lentiviral and RNAi

Expression

Mammalian

Available since

April 10, 2013

Availability

Academic Institutions and Nonprofits only