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pSI myc Nup53xl

Plasmid

#86001

Purpose

mammalian expression of myc tagged Xenopus laevis Nup53

Depositor

Wolfram Antonin

Article

Vollmer et al EMBO J. 2012 Oct 17;31(20):4072-84. doi:

Insert

Xenopus laevis Nup53 (nup35.L Frog)

Tags

myc

Expression

Mammalian

Mutation

codon optimzed for bacterial expression

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag 3B/myc-M87L195V

Plasmid

#87721

Purpose

Expresses myc-M87L195V spastin isoform in mammalian cells

Depositor

Peter Baas

Article

Solowska et al Hum Mol Genet. 2010 Jul 15;19(14):2767-7

Insert

SPAST (SPAST Human)

Tags

Myc

Expression

Mammalian

Mutation

M87 isoform (deletion of nt 1-490 - 5'UTR an…

Promoter

CMV

Available Since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag/S46X without 5'UTR

Plasmid

#87730

Purpose

Expresses S46X spastin M1 and M87 in mammalian cells

Depositor

Peter Baas

Article

Solowska et al Hum Mol Genet. 2010 Jul 15;19(14):2767-7

Insert

SPAST (SPAST Human)

Expression

Mammalian

Mutation

S46X; 5'UTR deleted (1-190 bp)

Promoter

CMV

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag/S44L without 5'UTR

Plasmid

#87728

Purpose

Expresses S44L spastin M1 and M87 in mammalian cells

Depositor

Peter Baas

Article

Solowska et al Hum Mol Genet. 2010 Jul 15;19(14):2767-7

Insert

SPAST (SPAST Human)

Expression

Mammalian

Mutation

S44L; 5'UTR deleted (1-190 bp)

Promoter

CMV

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag/L195V without 5'UTR

Plasmid

#87726

Purpose

Expresses L195V spastin M1 and M87 in mammalian cells

Depositor

Peter Baas

Article

Solowska et al Hum Mol Genet. 2010 Jul 15;19(14):2767-7

Insert

SPAST (SPAST Human)

Expression

Mammalian

Mutation

L195V; 5'UTR deleted (1-190 bp)

Promoter

CMV

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag/E112K without 5'UTR

Plasmid

#87724

Purpose

Expresses E112K spastin M1 and M87 in mammalian cells

Depositor

Peter Baas

Article

Solowska et al Hum Mol Genet. 2010 Jul 15;19(14):2767-7

Insert

SPAST (SPAST Human)

Expression

Mammalian

Mutation

E112K; 5'UTR deleted (1-190 bp)

Promoter

CMV

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag 3B/myc-M87E112K

Plasmid

#87720

Purpose

Expresses myc-M87E112K spastin isoform in mammalian cells

Depositor

Peter Baas

Article

Solowska et al Hum Mol Genet. 2010 Jul 15;19(14):2767-7

Insert

SPAST (SPAST Human)

Tags

Myc

Expression

Mammalian

Mutation

M87 isoform (deletion of nt 1-490 - 5'UTR an…

Promoter

CMV

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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pSB700 lenti gRNA Cerulean-zhang2.0-Weiss-chimera2

Plasmid

#79380

Purpose

Cas9 gRNA variant to recruit ms2 proteins

Depositor

George Church

Article

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

Chimera 2

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pWHI2-HYG

Plasmid

#80589

Purpose

CEN plasmid expressing full-length WHI2 from S.cerevisiae S288C background under its native promoter

Depositor

John McCusker

Article

Vijayraghavan et al Genetics. 2018 Nov 29. pii: genetics.118

Insert

WHI2 (WHI2 Budding Yeast)

Expression

Yeast

Promoter

WHI2 native promoter

Available Since

July 28, 2016

Availability

Academic Institutions and Nonprofits only
hFLN29G37-end/pCMV-14-FLAG

Plasmid

#73708

Purpose

Express human TRAFD1 (FLN29) from G37 to end in mammalian cells

Depositor

Paul Odgren

Article

Witwicka et al PLoS One. 2015 May 19;10(5):e0127537. do

Insert

TRAFD1 (TRAFD1 Human)

Tags

3xFlag

Expression

Mammalian

Mutation

deletion of first 36 aa from full lenght TRAFD1

Available Since

March 25, 2016

Availability

Academic Institutions and Nonprofits only
hFLN29 Q211-end/pCMV-14-FLAG

Plasmid

#73712

Purpose

Express human TRAFD1 (FLN29) from Q211 to end in mammalian cells

Depositor

Paul Odgren

Article

Witwicka et al PLoS One. 2015 May 19;10(5):e0127537. do

Insert

TRAFD1 (TRAFD1 Human)

Tags

3xFlag

Expression

Mammalian

Mutation

deletion of first 210 aa from full lenght TRAFD1

Available Since

March 15, 2016

Availability

Academic Institutions and Nonprofits only
hFLN29 L175-end/pCMV-14-FLAG

Plasmid

#73711

Purpose

Express human TRAFD1 (FLN29) from L175 to end in mammalian cells

Depositor

Paul Odgren

Article

Witwicka et al PLoS One. 2015 May 19;10(5):e0127537. do

Insert

TRAFD1 (TRAFD1 Human)

Tags

3xFlag

Expression

Mammalian

Mutation

deletion of first 174 aa from full lenght TRAFD1

Available Since

March 15, 2016

Availability

Academic Institutions and Nonprofits only
hFLN29 G117-end/pCMV-14-FLAG

Plasmid

#73710

Purpose

Express human TRAFD1 (FLN29) from G117 to end in mammalian cells

Depositor

Paul Odgren

Article

Witwicka et al PLoS One. 2015 May 19;10(5):e0127537. do

Insert

TRAFD1 (TRAFD1 Human)

Tags

3xFlag

Expression

Mammalian

Mutation

deletion of first 116 aa from full lenght TRAFD1

Available Since

March 15, 2016

Availability

Academic Institutions and Nonprofits only
hFLN29 C60-end/pCMV-14-FLAG

Plasmid

#73709

Purpose

Express human TRAFD1 (FLN29) fromC60 to end in mammalian cells

Depositor

Paul Odgren

Article

Witwicka et al PLoS One. 2015 May 19;10(5):e0127537. do

Insert

TRAFD1 (TRAFD1 Human)

Tags

3xFlag

Expression

Mammalian

Mutation

deletion of first 59 aa from full lenght TRAFD1

Available Since

March 15, 2016

Availability

Academic Institutions and Nonprofits only
pSFFV-Cx45

Plasmid

#69808

Purpose

mammalian expression of chick Cx45

Depositor

Richard Veenstra

Article

Veenstra et al Circ Res. 1992 Nov;71(5):1277-83.

Insert

Cx45 (GJC1 Chicken)

Expression

Mammalian

Available Since

Feb. 18, 2016

Availability

Academic Institutions and Nonprofits only
MT583

Plasmid

#63114

Purpose

The gDNA construct is designed for arabidopsis ecotype Est-1 ACD6 expression in other genetic backgrounds.

Depositor

Detlef Weigel

Article

Todesco et al Nature. 2010 Jun 3;465(7298):632-6. doi:

Insert

ACD6 (Accelerated Cell Death 6)

Expression

Plant

Available Since

Sept. 9, 2015

Availability

Academic Institutions and Nonprofits only

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MT79

Plasmid

#63112

Purpose

The artificial microRNA is designed for down-regulating expression of ACD6 mRNA.

Depositor

Detlef Weigel

Article

Todesco et al Nature. 2010 Jun 3;465(7298):632-6. doi:

Insert

amiR-ACD6

Expression

Plant

Available Since

Sept. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pCRII-Sema4b-ISH-2kb

Plasmid

#68030

Purpose

Contains 2kb cDNA fragment for probe synthesis for mRNA in situ hybridization, species: mouse

Depositor

Roland Friedel

Article

Maier et al Mol Cell Neurosci. 2011 Feb;46(2):419-31

Insert

Semaphorin-4B (Sema4b Mouse)

Use

Mrna in situ hybridization probe

Available Since

Aug. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pCRII-Sema4f-ISH-2kb

Plasmid

#68033

Purpose

Contains 2kb cDNA fragment for probe synthesis for mRNA in situ hybridization, species: mouse

Depositor

Roland Friedel

Article

Maier et al Mol Cell Neurosci. 2011 Feb;46(2):419-31

Insert

Semaphorin-4F (Sema4f Mouse)

Use

Mrna in situ hybridization probe

Available Since

Aug. 27, 2015

Availability

Academic Institutions and Nonprofits only

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NET33 pEGFP-N2 (594)

Plasmid

#61987

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

Insert

NET33 (SCARA5 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 17, 2015

Availability

Academic Institutions and Nonprofits only