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Plasmid#201952PurposeMammalian expression plasmid of high-fidelity SpCas9 variant SpCas9-HF1 with T2A-EGFP and cloning backbone for sgRNADepositorInsertMammalian expression plasmid of high-fidelity SpCas9 variant SpCas9-HF1 with T2A-EGFP and cloning backbone for sgRNA
UseCRISPRTags3xHA, NLS, and T2A-EGFPExpressionMammalianMutationPromoterCbhAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pINDUCER20-hMYOD1-3xHA (#730)
Plasmid#78328PurposeDoxycycline-inducible lentiviral overexpression of human MYOD1 ORF with a 3xHA tagDepositorInsertMYOD1 (MYOD1 Human)
UseLentiviralTags3x HA tagExpressionMutationPromoterTRE2Available sinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
qTAG-N-Blast-3xHA-LMNB1
Plasmid#207778PurposeDonor template for Blast-2A-3xHA insertion into the N-terminus of the LMNB1 locus. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770DepositorInsertLMNB1 Homology Arms flanking a Blast-3xHA Cassette (LMNB1 Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterAvailable sinceNov. 29, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pML340_RAB11A-N-term_XTEN80-mEGFP-3xHA_pTMPLT
Plasmid#227907PurposeHomology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.DepositorInsertRAB11A homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
piggybac-Syn-mScn8a-3xHA
Plasmid#182555PurposeExpresses Nav1.6 tagged with 3xHADepositorInsertScn8a (Scn8a Mouse)
UseTags3xHAExpressionMammalianMutationPromoterSynAvailable sinceMarch 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCS2+N-3xHA Cloning Vector
Plasmid#196653PurposeN-terminal 3xHA mammalian expression plasmidDepositorTypeEmpty backboneUseTags3xHAExpressionMammalianMutationPromoterCMVAvailable sinceMarch 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUAST-attB-3xHA-V5-RpL10Ab
Plasmid#125223Purpose3xHA-V5 tagged RpL10Ab for expression from UAS promoter to TRAP translatome from specific cell types defined by GAL4 expressionDepositorInsertRpL10Ab (RpL10Ab Fly)
UseTags3x HA, V5ExpressionInsectMutationPromoterAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUTKan-3xHA-GFP-TOM5
Plasmid#130674PurposeUbiquitously expresses 3xHA-GFP-TOM5 in the outer mitochondrial membrane of plantaDepositorInsertTranslocase of outer mitochondrial membrane 5 (TOM5 Mustard Weed)
UseTags3xHA and sGFPExpressionPlantMutationPromoterUbiquitin10Available sinceSept. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSLIK 3xHA-MKK6-EE neo
Plasmid#47546PurposeLentiviral expression vector for an inducible 3xHA-tagged constitutively active MKK6 mutantDepositorInsertMKK6-EE (MAP2K6 Human)
UseLentiviralTags3XHAExpressionMammalianMutationS207E, T211EPromoterAvailable sinceSept. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1 gfaABC1D-3xHA-SAPAP3
Plasmid#190200PurposeExpresses SAPAP3 protein fused with an HA tag at the N-terminus in astrocytesDepositorInsert3xHA-SAPAP3 (Dlgap3 Mouse)
UseAAVTagsHAExpressionBacterialMutationPromotergfaABC1DAvailable sinceApril 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pINDUCER20-hMYOG-3xHA (#733)
Plasmid#78342PurposeDoxycycline-inducible lentiviral overexpression of human MYOG ORF with a 3xHA tagDepositorInsertMYOG (MYOG Human)
UseLentiviralTags3x HA tagExpressionMutationPromoterTRE2Available sinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
FUGW-3xHA-KCNJ6-mCherry
Plasmid#197032PurposeExpress 3xHA-tagged human KCNJ6 (Girk2), co-expressed with mCherry, from UbiC promoterDepositorInsertKCNJ6 (KCNJ6 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
UBQ10:sXVE:S10-(MCS)-3xHA
Plasmid#108178PurposeBinary vectors used for the inducible expression of tripartite split-sfGFP components (with a 3xHA tag) in plantsDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionPlantMutationPromoterUBQ10:sXVE:Available sinceMay 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
CAG.3xHalo-GFP-SPC25
Plasmid#176206PurposeKinetochore anchor for TMP-Halo dimerization systemDepositorInsert3xHalo-GFP-SPC25
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pINDUCER20-hMYF5-3xHA (#732)
Plasmid#78332PurposeDoxycycline-inducible lentiviral overexpression of human MYF5 ORF with a 3xHA tagDepositorInsertMYF5 (MYF5 Human)
UseLentiviralTags3x HA tagExpressionMutationPromoterTRE2Available sinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAav-MCS-PQS2-3xHA
Plasmid#84917PurposerAAV-based template for genome engineering of protein C-termini containing PQS2 and 3xHA tags and a selection cassetteDepositorInsertLOX-PGK-NEO-LOX
UseAAVTagsPQS2 3xHAExpressionMutationPromotermPGKAvailable sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMXs-hs-3xHA-delta6xNHL-LIN-41
Plasmid#52722Purposeretroviral expression of human LIN-41 / TRIM71 containing deletion of the 6xNHL repeats and 3 N-terminal HA tagsDepositorInsertLIN-41 (TRIM71 Human)
UseRetroviralTags3xHAExpressionMammalianMutationdeleted amino acids 593–868PromoterAvailable sinceJune 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
prk5-TMEM192-3xHA PM mutant
Plasmid#149506PurposeTransient expression of human TMEM192. Dileucine motifs LL23-24 and LL28-29 have been mutated to AA.DepositorInsertTMEM192 (TMEM192 Human)
UseTransient expressionTags3XHAExpressionMammalianMutationp.LL23-24AA, p.LL28-29AAPromoterCMVAvailable sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMXs-hs-3xHA-deltaRING-LIN-41
Plasmid#52718Purposeretroviral expression of human LIN-41 / TRIM71 containing a RING domain deletion and 3 N-terminal HA tagsDepositorInsertLIN-41 (TRIM71 Human)
UseRetroviralTags3xHAExpressionMammalianMutationdeleted amino acids 12-91PromoterAvailable sinceJune 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAav-MDM2-PQS2-3xHA
Plasmid#84886PurposerAAV-based template for genome engineering of the MDM2 protein C-terminus containing PQS2 and 3xHA tags and a selection cassetteDepositorUseAAVTagsPQS2 3xHAExpressionMutationPromoternoAvailable sinceDec. 7, 2016AvailabilityAcademic Institutions and Nonprofits only