We narrowed to 1,198 results for: MCCA

1. pNL4-3 envFS eGFP NoStarts

   Plasmid

   #101342

   Purpose

   All ATGs from the start of gag to NC mutated to ATC except the first which was mutated to ACG

   Depositor

   Jeremy Luban

   Article

   McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

   Insert

   NL4-3
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   No methonines in Gag

   Promoter

   Available since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBS NL4-3 envFS eGFP RT-D185K/D186L

   Plasmid

   #101332

   Purpose

   pBS NL4-3 envFS eGFP with mutation that disrupts RT catalytic activity

   Depositor

   Jeremy Luban

   Article

   McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

   Insert

   NL4-3 envFS
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   reverse transcriptase D185K/D186L

   Promoter

   Available since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJES103

   Plasmid

   #135410

   Purpose

   E. coli 'blaTEM-1 with M. tuberculosis mpt63 signal sequence under the control of hsp60 promoter that can be used as a reporter

   Depositor

   Miriam Braunstein

   Article

   McCann et al Microbiology. 2007 Oct;153(Pt 10):3350-9

   Insert

   blaTEM-1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   E. coli 'blaTEM-1 with M. tuberculosis mpt63…

   Promoter

   hsp60

   Available since

   Feb. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pALPS tat-P2A-rev

   Plasmid

   #101331

   Purpose

   Lentivector expressing codon optimized NL4-3 tat and rev linked by P2A peptide coding sequence (GSGATNFSLLKQAGDVEENPGP)

   Depositor

   Jeremy Luban

   Article

   McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

   Insert

   NL4-3 tat and rev
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBS NL4-3 envFS eGFP Δnef/U3

   Plasmid

   #101346

   Purpose

   Deletion from NL4-3 nt 8911-9022 and 9088-9377. This deletes nef and U3 LTR sequences

   Depositor

   Jeremy Luban

   Article

   McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

   Insert

   NL4-3
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   delta nef/LTR

   Promoter

   Available since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pALPS_3'LTR GFP@gag start SFFV-

   Plasmid

   #101337

   Purpose

   Repaired U3 allows LTR-based transcription by the provirus with GFP as a marker for expression. WPRE was deleted.

   Depositor

   Jeremy Luban

   Article

   McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

   Insert

   GFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pALPS_3'LTR GFP@gag start SFFV rev

   Plasmid

   #101339

   Purpose

   LTR drives GFP and internal SFFV promoter drives codon optimized rev

   Depositor

   Jeremy Luban

   Article

   McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

   Insert

   GFP, rev
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBS NL4-3 envFS eGFP Δgag/pol

   Plasmid

   #101343

   Purpose

   Deletion from the start of gag until 229 bases before the cPPT

   Depositor

   Jeremy Luban

   Article

   McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

   Insert

   NL4-3
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   delta GagPol

   Promoter

   Available since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pALPS_3'LTR GFP@gag start SFFV tat

   Plasmid

   #101338

   Purpose

   LTR drives GFP and internal SFFV promoter drives codon optimized tat

   Depositor

   Jeremy Luban

   Article

   McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

   Insert

   GFP, tat
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. DDX5 WT V5

    Plasmid

    #199576

    Purpose

    Expresses WT DDX5 cloned into pLenti puro

    Depositor

    Michael Kastan

    Article

    McCann et al Radiat Res. 2023 Apr 1;199(4):406-421. d

    Insert

    DDX5 (DDX5 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pALPS_3'LTR GFP@gag start SFFV tat-P2A-rev

    Plasmid

    #101340

    Purpose

    LTR drives GFP and internal SFFV promoter drives codon optimized tat and rev.

    Depositor

    Jeremy Luban

    Article

    McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

    Insert

    GFP, tat, rev
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. FLAG SMG1 D2335A/N2340K

    Plasmid

    #199575

    Purpose

    Expresses FLAG-tagged kinase dead SMG1

    Depositor

    Michael Kastan

    Article

    McCann et al Radiat Res. 2023 Apr 1;199(4):406-421. d

    Insert

    SMG1 (SMG1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    D2335A and N2340K

    Promoter

    CMV

    Available since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pStr-KDEL-IRES-ST-mCherry

    Plasmid

    #110727

    Purpose

    Expresses an Str-KDEL Hook for the RUSH system and separately Sialyltransferase-mCherry

    Depositor

    David Stephens

    Article

    McCaughey et al J Cell Biol. 2018 Dec 26. pii: jcb.20180

    Inserts

    Str-KDEL
    STase sialyltransferase (ST6GAL1 Human)
    

    Use

    Tags

    Str Streptavidin binding peptide (SBP) and mCherr…

    Expression

    Mammalian

    Mutation

    contains only amino acids 1 to 43 of ST

    Promoter

    CMV and IRES

    Available since

    Jan. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBS NL4-3 envFS eGFP Δrev

    Plasmid

    #101348

    Purpose

    All mutations in rev are silent with respect to the tat reading frame. Start ATG->ACG and nts 68-71 were mutated AGC->TCA to change tyrosine to a stop.

    Depositor

    Jeremy Luban

    Article

    McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

    Insert

    NL4-3
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    delta rev

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. FLAG SMG1 T3550A/S3556A

    Plasmid

    #199574

    Purpose

    Expresses FLAG-tagged mutated SMG1

    Depositor

    Michael Kastan

    Article

    McCann et al Radiat Res. 2023 Apr 1;199(4):406-421. d

    Insert

    SMG1 (SMG1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    T3550A and S3556A

    Promoter

    CMV

    Available since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSC101 NL4-3 envFS Δrev ΔRRE RTEm26CTE

    Plasmid

    #101351

    Purpose

    HIV-1 delta Rev was cloned into pSC101 and modified to include an RTEm26CTE element in order to utilize the NXF1 RNA export pathway. The RRE was also mutated.

    Depositor

    Jeremy Luban

    Article

    McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

    Insert

    NL4-3
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. GFP SMG1 T3550A/S3556A

    Plasmid

    #199572

    Purpose

    Expresses mutated SMG1 fused to GFP

    Depositor

    Michael Kastan

    Article

    McCann et al Radiat Res. 2023 Apr 1;199(4):406-421. d

    Insert

    SMG1 (SMG1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    T3550A and S3556A

    Promoter

    CMV

    Available since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAIP hIL4 co

    Plasmid

    #74169

    Purpose

    Lentiviral vector for expression of human codon optimized IL4 with puromycin resistance.

    Depositor

    Jeremy Luban

    Article

    McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

    Insert

    IL4 (IL4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    June 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAIP hIL2 co

    Plasmid

    #90513

    Purpose

    Lentiviral vector for expression of human codon optimized IL2 with puromycin resistance.

    Depositor

    Jeremy Luban

    Article

    McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

    Insert

    IL2 (IL2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    May 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAIP hGMCSF co

    Plasmid

    #74168

    Purpose

    Lentiviral vector for expression of human codon optimized GMCSF with puromycin resistance.

    Depositor

    Jeremy Luban

    Article

    McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

    Insert

    GMCSF (CSF2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    June 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge