We narrowed to 2,195 results for: Xpa

1. pDEST27-GST-ATXN1 FL(84Q)-S776A

   Plasmid

   #21758

   Depositor

   Huda Zoghbi

   Article

   Lim et al Nature. 2008 Apr 10. 452(7188):713-8.

   Insert

   Ataxin-1 (ATXN1 Human)
   

   Tags

   GST

   Expression

   Mammalian

   Mutation

   insert contains a stretch of 84 Q(Glutamines) and…

   Promoter

   CMV

   Available Since

   July 22, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pHR-HOXD13(+7A)-IDR-YFP-NLS

   Plasmid

   #145278

   Purpose

   Mammalian Expression of Nuclear HOXD13 (+7A) IDR-YFP with SV40 NLS

   Depositor

   Denes Hnisz

   Article

   Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

   Insert

   HOXD13(+7A)-IDR (HOXD13 Human)
   

   Expression

   Mammalian

   Mutation

   A-repeat expanded

   Available Since

   Sept. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET-45b-HOXA13(+7A)-IDR-mCherry

   Plasmid

   #145285

   Purpose

   Bacterial Expression of 6xHis HOXA13(+7A)IDR mCherry Fusion Protein

   Depositor

   Denes Hnisz

   Article

   Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

   Insert

   HOXA13(+7A)-IDR (HOXA13 Human)
   

   Expression

   Bacterial

   Mutation

   A-repeat expanded

   Available Since

   Sept. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCFP-LacI-HOXD13(+10A)-IDR

   Plasmid

   #145257

   Purpose

   Expresses fusion of ECFP-LacI and HOXD13+10A IDR

   Depositor

   Denes Hnisz

   Article

   Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

   Insert

   HOXD13(+10A)-IDR (HOXD13 Human)
   

   Expression

   Mammalian

   Mutation

   A-repeat expanded

   Available Since

   Sept. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCFP-LacI-HOXD13(+7A)-IDR

   Plasmid

   #145256

   Purpose

   Expresses fusion of ECFP-LacI and HOXD13+7A IDR

   Depositor

   Denes Hnisz

   Article

   Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

   Insert

   HOXD13(+7A)-IDR (HOXD13 Human)
   

   Expression

   Mammalian

   Mutation

   A-repeat expanded

   Available Since

   Sept. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGAL4-DBD-HOXA13+7A-IDR

   Plasmid

   #145239

   Purpose

   Expresses fusion of GAL4 DNA-binding domain and HOXA13+7A IDR

   Depositor

   Denes Hnisz

   Article

   Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

   Insert

   HOXA13+7A-IDR (HOXA13 Human)
   

   Expression

   Mammalian

   Mutation

   A-repeat expanded

   Available Since

   Sept. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAJE3-E7

   Plasmid

   #214359

   Purpose

   Expresses the 2nd-generation "E7" Tet2 Methanocaldococcus jannaschii (Mj) aminoacyl tRNA synthetase/tRNA pair for encoding Tet2 noncanonical amino acids into TAG sites of proteins

   Depositor

   Ryan Mehl

   Article

   Eddins et al Bioconjug Chem. 2023 Dec 20;34(12):2243-

   Inserts

   2nd generation Tet2 "E7" aminoacyl tRNA synthetase
   Lpp promoted M. jannaschii tRNA
   Aminoglycoside-3''-adenyltransferase (Spectinomycin/streptomycin Resistance Gene)
   Orthogonal "D4II" ColE1 origin
   

   Expression

   Bacterial

   Promoter

   AmpR and lpp

   Available Since

   March 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAS_4xMma PylT_FLAG-AbKRS-chIPYE

   Plasmid

   #140020

   Purpose

   Plasmid with 4xMmaPylT cassette and chimeric Mma/Mba PylRS for amber suppression and incorporation of photo-crosslinking-lysine; for transient or stable piggyBac-mediated integration

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

   Insert

   AbKRS-chIPYE (pylS Methanosarcina mazei and Methanosarcina bakeri)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   1-149 MbPylRS IPYE (V31I, T56P, H62Y, A100E) + 18…

   Promoter

   EF1

   Available Since

   June 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHR-HOXD13(+8A)-IDR-mCherry-CRY2-NLS

   Plasmid

   #145262

   Purpose

   Mammalian Expression of Nuclear Opto HOXD13(+8A) IDR with SV40 NLS

   Depositor

   Denes Hnisz

   Article

   Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

   Insert

   HOXD13(+8A)-IDR (HOXD13 Human)
   

   Expression

   Mammalian

   Mutation

   A-repeat expanded

   Available Since

   Sept. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pRaGE Pyl TAG GFP Y35TAG

    Plasmid

    #160041

    Purpose

    Encodes for MmPyl tRNA synthetase/tRNA pair and for GFP reporter with TAG mutation at site 35, for unnatural amino acid incorporation into the GFP protein in Vibrio natriegens.

    Depositor

    Lital Alfonta

    Article

    Ozer et al Front Bioeng Biotechnol. 2021 Feb 26;9:5

    Inserts

    Pyrrolysyl tRNA synthetase (Methanosarcina mazei)
    Pyrrolysyl tRNA(cua) (Methanosarcina mazei)
    deGFP
    

    Use

    Synthetic Biology

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Changed tyrosine 35 to TAG stop-codon (Site numbe…

    Promoter

    P70b promoter and T500 terminator, Vibrio natrieg…

    Available Since

    May 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMega-MaPylRS

    Plasmid

    #200225

    Purpose

    The plasmid encodes an orthogonal aminoacyl-tRNA synthetase/tRNA-CUA pair for amber codon suppression with Nε-Boc-lysine.

    Depositor

    Alanna Schepartz

    Article

    Fricke et al Nat Chem. 2023 Jun 1. doi: 10.1038/s4155

    Inserts

    Pyrrolysyl-tRNA synthetase
    pyrrolysyl-tRNA
    

    Tags

    none

    Expression

    Bacterial

    Mutation

    none

    Promoter

    proK-lacO and tacI

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEGFP-C1-AR Q0

    Plasmid

    #86427

    Purpose

    Fluorescent human androgen receptor (fused to EGFP) with deleted polyglutamine region

    Depositor

    Michael Mancini

    Article

    Stenoien et al Hum Mol Genet. 1999 May . 8(5):731-41.

    Insert

    androgen receptor (AR) (AR Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Deletion of the poly-glutamine expansion

    Promoter

    CMV

    Available Since

    April 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMega-MaFRSA

    Plasmid

    #200226

    Purpose

    The plasmid encodes an orthogonal aminoacyl-tRNA synthetase/tRNA-CUA pair for amber codon suppression with meta-trifluoromethylphenylalanine..

    Depositor

    Alanna Schepartz

    Article

    Fricke et al Nat Chem. 2023 Jun 1. doi: 10.1038/s4155

    Inserts

    Pyrrolysyl-tRNA synthetase
    pyrrolysyl-tRNA
    

    Tags

    none

    Expression

    Bacterial

    Mutation

    mutated Asparagine 166 to Alanine and Valine 168 …

    Promoter

    proK-lacO and tacI

    Available Since

    June 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHR-HOXD13(+7A)-IDR-mCherry-CRY2-NLS

    Plasmid

    #145261

    Purpose

    Mammalian Expression of Nuclear Opto HOXD13(+7A) IDR with SV40 NLS

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    HOXD13(+7A)-IDR (HOXD13 Human)
    

    Expression

    Mammalian

    Mutation

    A-repeat expanded

    Available Since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET-45b-HOXD13(+10A)-IDR-mCherry

    Plasmid

    #145282

    Purpose

    Bacterial Expression of 6xHis HOXD13 (+10A) IDR mCherry Fusion Protein

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    HOXD13(+10A)-IDR (HOXD13 Human)
    

    Expression

    Bacterial

    Mutation

    A-repeat expanded

    Available Since

    Sept. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHR-HOXD13(+9A)-IDR-mCherry-CRY2-NLS

    Plasmid

    #145263

    Purpose

    Mammalian Expression of Nuclear Opto HOXD13(+9A) IDR with SV40 NLS

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    HOXD13(+9A)-IDR (HOXD13 Human)
    

    Expression

    Mammalian

    Mutation

    A-repeat expanded

    Available Since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pHR-HOXA13(+7A)-IDR-mCherry-CRY2-NLS

    Plasmid

    #145266

    Purpose

    Mammalian Expression of Nuclear Opto HOXA13(+7A) IDR with SV40 NLS

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    HOXA13(+7A)-IDR (HOXA13 Human)
    

    Expression

    Mammalian

    Mutation

    A-repeat expanded

    Available Since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHR-TBP(53Q)-IDR-mCherry-Cry2-NLS

    Plasmid

    #145270

    Purpose

    Mammalian Expression of Nuclear Opto TBP(53Q) IDR with SV40 NLS

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    TBP(53Q)-IDR (TBP Human)
    

    Expression

    Mammalian

    Mutation

    Q-repeat expanded

    Available Since

    Sept. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. CS-TRE-BclXL-Ubc-rtTA

    Plasmid

    #198189

    Purpose

    Generation of Expandable megakaryocyte cell lines from human iPS cells

    Depositor

    Hiromitsu Nakauchi

    Article

    Nakamura et al Cell Stem Cell. 2014 Apr 3;14(4):535-48.

    Insert

    hBCLXL (BCL2L1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    May 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. CS-TRE-BMI1-Ubc-rtTA

    Plasmid

    #198191

    Purpose

    Generation of Expandable megakaryocyte cell lines from human iPS cells

    Depositor

    Hiromitsu Nakauchi

    Article

    Nakamura et al Cell Stem Cell. 2014 Apr 3;14(4):535-48.

    Insert

    hBMI1 (BMI1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    May 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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