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Showing: 101 - 120 of 23744 results

  2. p-dCas9-LSD1-Hygro

    Plasmid
    #104406
    Purpose
    transient expression of dCas9-LSD1 fusion protein
    Depositor
    Stephan Beck
    Insert
    LSD1 (KDM1A Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    IRATp970A0364D (Source Bioscience) has a P405H ch…
    Promoter
    CMV promoter
    Available since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    104406-plasmid-map-sequence-id-204465

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  3. p-dCas9-VP64-Hygro

    Plasmid
    #104405
    Purpose
    transient expression of dCas9-VP64 fusion protein
    Depositor
    Stephan Beck
    Insert
    VP64
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV promoter
    Available since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    104405-plasmid-map-sequence-id-204462

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  4. 3xFLAG-dCas9/p-bacteria

    Plasmid
    #64325
    Purpose
    Expresses 3xFLAG-dCas9 in E. coli in a tetracycline-dependent manner for enChIP analysis to purify specific genomic regions of interest.
    Depositor
    Hodaka Fujii
    Article
    Fujita et al BMC Res Notes. 2018 Jun 14;11(1):387. do
    Insert
    3xFLAG-dCas9
    Use
    CRISPR; Tetracycline-inducible
    Tags
    3xFLAG
    Expression
    Bacterial
    Mutation
    D10A, H840A
    Promoter
    pLtetO-1
    Available since
    Aug. 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    64325-plasmid-map-sequence-id-107969

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  5. LUC01 (L/Sec/P)

    Plasmid
    #112144
    Purpose
    Mammalian expression vector to test the effect of substituting selenocysteine for Cys on luciferase enzyme activity
    Depositor
    Paul Copeland
    Article
    Mehta et al J Biol Chem. 2004 Sep 3;279(36):37852-9.
    Insert
    Luciferase coding region with Cysteine 258 mutated to selenocysteine (U) and wild-type PHGPx SECIS in the 3' UTR (Gpx4 Rat, Photinus pyralis)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Cysteine 258 mutated to selenocysteine (U)
    Promoter
    Available since
    July 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    112144-plasmid-map-sequence-id-214867

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  7. LUC05 (L/Cys/P)

    Plasmid
    #112146
    Purpose
    Mammalian expression vector to test the effect of substituting selenocysteine for Cys on luciferase enzyme activity
    Depositor
    Paul Copeland
    Article
    Mehta et al J Biol Chem. 2004 Sep 3;279(36):37852-9.
    Insert
    Luciferase coding region with wild type Cysteine 258 and wild-type PHGPx SECIS in the 3' UTR (Gpx4 Rat, Photinus pyralis)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    none
    Promoter
    Available since
    July 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    112146-plasmid-map-sequence-id-214888

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  8. ER-P-TAG (pGS594)

    Plasmid
    #160039
    Purpose
    pLV-A4-UCOE-EF1a-CP450TMD-APEX2-3xHA-IRES-BLAS-2A-SNAP-WPRE-A2
    Depositor
    David Sabatini
    Insert
    CP450TMD-APEX2-3xHA
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    EF1
    Available since
    March 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    160039-plasmid-map-sequence-id-327043

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  9. p-spTorA-GFP-H6C

    Plasmid
    #168517
    Purpose
    GFP with the signal peptide of TorA (spTorA) in pBAD24. Includes a C-terminal HHHHHHC (6xHisC) extension.
    Depositor
    Siegfried Musser
    Article
    Bageshwar et al PLoS One. 2021 Sep 9;16(9):e0256715. doi
    Insert
    spTorA-GFP-H6C
    Use
    Tags
    6xHisC tag and spTorA (signal peptide of E. coli …
    Expression
    Bacterial
    Mutation
    The mut3 GFP variant with 5 additional mutations …
    Promoter
    araBAD
    Available since
    Oct. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    168517-plasmid-map-sequence-id-346120

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  11. p-mCherry-C1-GtACR1

    Plasmid
    #85464
    Purpose
    Natural anion conducting Channelrhodopsin with cyan absorption. Closing kinetics 140ms. High conductivity. Codon optimized for mammalian expression (human/mouse)
    Depositor
    Peter Hegemann
    Article
    Wietek et al J Biol Chem. 2016 Feb 19;291(8):4121-7.
    Insert
    Synthetic construct ACR1 gene
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV (+enhancer)
    Available since
    Jan. 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    85464-plasmid-map-sequence-id-170772

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  12. p-mCherry-C1-GtACR2

    Plasmid
    #85463
    Purpose
    Natural anion conducting Channelrhodopsin with blue absorption. Closing kinetics 60ms. High conductivity. Codon optimized for mammalian expression (human/mouse)
    Depositor
    Peter Hegemann
    Article
    Wietek et al J Biol Chem. 2016 Feb 19;291(8):4121-7.
    Insert
    Synthetic construct ACR2 gene
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV (+enhancer)
    Available since
    Jan. 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    85463-plasmid-map-sequence-id-170777

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  13. p-mCherry-N1-ChloC

    Plasmid
    #85461
    Purpose
    Historical plasmid. For inhibition of neuronal activity, #66709 is a light-gated chloride channel with improved anion selectivity (iChloC)
    Depositor
    Peter Hegemann
    Article
    Wietek et al Science. 2014 Mar 27.
    Insert
    ChloC
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    E90R, T159C
    Promoter
    CMV (+enhancer)
    Available since
    Jan. 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    85461-plasmid-map-sequence-id-170778

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  16. CoV2-N-P.1

    Plasmid
    #177959
    Purpose
    Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (P80R, R203K, G204R). Used for generating RNA packaging virus-like particles.
    Depositor
    Jennifer Doudna
    Article
    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11
    Insert
    SARS-CoV-2 Nucleocapsid (P80R, R203K, G204R) (N )
    Use
    Tags
    Expression
    Mammalian
    Mutation
    P80R,R203K,G204R
    Promoter
    Available since
    Nov. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    177959-plasmid-map-sequence-id-348182

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  19. p-mCherry-C1-iChR2++

    Plasmid
    #98172
    Purpose
    artificial anion conducting channelrhodopsin (aACR). Activation with blue light. Codon optimized for mammalian expression.
    Depositor
    Peter Hegemann
    Article
    Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.
    Insert
    Synthetic construct iChR2++ gene
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    A59S, E83N, E90Q, E101S, Q117R, E123S, T159C, V24…
    Promoter
    CMV (+enhancer)
    Available since
    July 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    98172-plasmid-map-sequence-id-182770

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  20. p-mCherry-C1-PsACR1

    Plasmid
    #85465
    Purpose
    Natural anion conducting Channelrhodopsin with red shifted absorption. Closing kinetics 80ms. Codon optimized for mammalian expression (human/mouse)
    Depositor
    Peter Hegemann
    Article
    Wietek et al J Biol Chem. 2016 Feb 19;291(8):4121-7.
    Insert
    Synthetic construct PsChR1 gene
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV (+enhancer)
    Available since
    Jan. 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    85465-plasmid-map-sequence-id-170779

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Showing: 101 - 120 of 23744 results