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pGEM_ZmaBOP2binsitu

Plasmid

#136532

Purpose

Template for generating in situ hybridization probe

Depositor

Junko Kyozuka

Article

Toriba et al Curr Biol. 2020 Feb 3;30(3):509-516.e3.

Insert

Zoysia matrella BLADE-ON-PETIOLE homolog2b

Use

Cloning vector

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pGEM_HcBOP1binsitu

Plasmid

#136530

Purpose

Template for generating in situ hybridization probe

Depositor

Junko Kyozuka

Article

Toriba et al Curr Biol. 2020 Feb 3;30(3):509-516.e3.

Insert

Houttuynia cordata BLADE-ON-PETIOLE homolog1b

Use

Cloning vector

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pUC57-fsr1

Plasmid

#179866

Purpose

Encodes the polyketide synthase Fsr1 from Fusarium vanettenii codon optimized for expression in Saccharomyces cerevisiae

Depositor

Jens Laurids Sørensen

Article

Bejenari et al J Microbiol Biol Educ. 2023 Nov 27;24(3)

Insert

Fusarubin polyketide synthase

Use

Synthetic Biology

Mutation

Codon optimized for expression in Saccharomyces c…

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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EF-hGPC3

Plasmid

#24670

Depositor

Jorge Filmus

Article

Gonzalez et al J Cell Biol. 1998 Jun 15. 141(6):1407-14

Insert

GPC3 (GPC3 Human)

Tags

HA

Expression

Mammalian

Available Since

May 6, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA5/FRT/TO GABARAP

Plasmid

#218427

Purpose

Expression of tagless GABARAP

Depositor

Dieter Willbold

Article

Uffing et al Autophagy Rep. 2024 May 16;3(1):2348899.

Insert

GABARAP (GABARAP Human)

Expression

Mammalian

Promoter

CMV

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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pK-eCFP

Plasmid

#176559

Purpose

Expression of eCFP for auxotrophic selection in the absence of histidine

Depositor

Markus Ralser

Article

Olin-Sandoval et al Nature. 2019 Aug;572(7768):249-253. doi:

Insert

eCFP

Expression

Yeast

Promoter

TDH1(GAP)

Available Since

Dec. 23, 2021

Availability

Academic Institutions and Nonprofits only

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ScSpe1 pET-20b(+)

Plasmid

#117145

Purpose

Expresses the His tagged Saccharomyces cerevisiae Ornithine Decarboxylase in Escherichia coli BL21

Depositor

Markus Ralser

Article

Olin-Sandoval et al Nature. 2019 Aug;572(7768):249-253. doi:

Insert

SPE1

Tags

Histidine

Expression

Bacterial

Promoter

T7

Available Since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO mTagBFP2-GABARAP

Plasmid

#212110

Purpose

Expression of N-terminally tagged GABARAP with mTagBFP2

Depositor

Dieter Willbold

Article

Uffing et al Autophagy Rep. 2024 May 16;3(1):2348899.

Insert

mTagBFP2-GABARAP (GABARAP Entacmaea quadricolor, Human)

Tags

mTagBFP2

Expression

Mammalian

Promoter

CMV

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO eYFP-GABARAP

Plasmid

#212111

Purpose

Expression of N-terminally tagged GABARAP with eYFP

Depositor

Dieter Willbold

Article

Uffing et al Autophagy Rep. 2024 May 16;3(1):2348899.

Insert

eYFP-GABARAP (GABARAP Aequorea victoria, Human)

Tags

eYFP

Expression

Mammalian

Promoter

CMV

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pCOA-Leu-FvPPT1

Plasmid

#179923

Purpose

Expression vector for heterologous expression of a polyketide synthase in S. cerevisiae. Carries and empty insertion site and the Fusarium verticillioides 4´-phosphopantetheinyltransferase PPT1

Depositor

Jens Laurids Sørensen

Article

Bejenari et al J Microbiol Biol Educ. 2023 Nov 27;24(3)

Insert

FvPPT1

Expression

Yeast

Mutation

Codon optimized for expression in Saccharomyces c…

Promoter

Yarrowia lipolytica EXP1

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO GABARAP(G116A)-mTagBFP2

Plasmid

#212112

Purpose

Expression of C-terminally tagged GABARAP(G116A) with mTagBFP2

Depositor

Dieter Willbold

Article

Uffing et al Autophagy Rep. 2024 May 16;3(1):2348899.

Insert

GABARAP(G116A)-mTagBFP2 (GABARAP Entacmaea quadricolor, Human)

Tags

mTagBFP2

Expression

Mammalian

Mutation

G116A (lipidation deficiency) of GABARAP

Promoter

CMV

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO mTagBFP2-GABARAP(G116A)-GABARAP

Plasmid

#212109

Purpose

Expression of N-terminally tagged GABARAP tandem construct with mTagBFP2

Depositor

Dieter Willbold

Article

Uffing et al Autophagy Rep. 2024 May 16;3(1):2348899.

Insert

mTagBFP2-GABARAP(G116A)-GABARAP (GABARAP Entacmaea quadricolor, Human)

Tags

mTagBFP2

Expression

Mammalian

Promoter

CMV

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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U6REST1_U6REST2.hPGK.BRN2.hPGK.Ascl1WPRE

Plasmid

#101852

Purpose

Direct reprogramming of adult fibroblasts to neurons

Depositor

Malin Parmar

Article

Drouin-Ouellet et al EMBO Mol Med. 2017 Aug;9(8):1117-1131. d

Insert

Ascl1, Brn2

Use

Lentiviral

Promoter

U6, PGK

Available Since

Oct. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pET-15b_LC3B

Plasmid

#73949

Purpose

For recombinant expression of human LC3B/MAP1LC3B in E. coli

Depositor

Dieter Willbold

Article

Ma et al J Biol Chem. 2013 Dec 27;288(52):37204-1

Insert

LC3B (MAP1LC3B Human)

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

March 25, 2016

Availability

Academic Institutions and Nonprofits only
pLKO.1-REST_sh_seq1_WPRE

Plasmid

#127573

Purpose

shRNA to knock down RE1-silencing transcription factor (REST)

Depositor

Malin Parmar

Article

Drouin-Ouellet et al EMBO Mol Med. 2017 Aug;9(8):1117-1131. d

Insert

shRNA for knock down of REST (REST Human)

Use

Lentiviral

Promoter

U6

Available Since

July 18, 2019

Availability

Academic Institutions and Nonprofits only

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pLKO.1REST_sh_seq2_WPRE

Plasmid

#127574

Purpose

shRNA to knock down RE1-silencing transcription factor (REST)

Depositor

Malin Parmar

Article

Drouin-Ouellet et al EMBO Mol Med. 2017 Aug;9(8):1117-1131. d

Insert

shRNA for knock down of REST (REST Human)

Use

Lentiviral

Promoter

U6

Available Since

July 18, 2019

Availability

Academic Institutions and Nonprofits only

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pGex-4T-2_LC3A

Plasmid

#73946

Purpose

For recombinant expression of human LC3A/MAP1LC3A in E. coli

Depositor

Dieter Willbold

Article

Ma et al J Biol Chem. 2013 Dec 27;288(52):37204-1

Insert

LC3A (MAP1LC3A Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

March 25, 2016

Availability

Academic Institutions and Nonprofits only
pGex-4T-2_GATE-16

Plasmid

#73518

Purpose

For recombinant expression of human GATE-16/GABARAPL2 in E. coli

Depositor

Dieter Willbold

Article

Ma et al J Biol Chem. 2013 Dec 27;288(52):37204-1

Insert

GATE-16 (GABARAPL2 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

March 25, 2016

Availability

Academic Institutions and Nonprofits only
pGex-4T-2_GEC1

Plasmid

#73945

Purpose

For recombinant expression of human GEC1/GABARAPL1 in E. coli

Depositor

Dieter Willbold

Article

Ma et al J Biol Chem. 2013 Dec 27;288(52):37204-1

Insert

GEC1 (GABARAPL1 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

March 25, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA5/FRT/TO GABARAP(G116A)-mCherry-GABARAP

Plasmid

#212113

Purpose

Expression of GABARAP split tandem protein with mCherry

Depositor

Dieter Willbold

Article

Uffing et al Autophagy Rep. 2024 May 16;3(1):2348899.

Insert

GABARAP(G116A)-mCherry-GABARAP (GABARAP Human, Discosoma sp.)

Tags

mCherry

Expression

Mammalian

Mutation

G116A (lipidation deficiency) of upstream GABARAP

Promoter

CMV

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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