We narrowed to 10,629 results for: t7

1. T7-VEE-OKS-iG

   Plasmid

   #58974

   Purpose

   RNA-based iPSC generation. VEE RNA replicon 3'ORF encoding OCT4, KLF4, SOX2 separated by 2A peptides followed by an IRES then GLIS1 and a second IRES and PuroR ORF

   Depositor

   Steven Dowdy

   Article

   Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

   Inserts

   Oct4 (POU5F1 Human)
   Klf4 (KLF4 Human)
   Sox2 (SOX2 Human)
   GLIS (GLIS1 Human)
   

   Use

   Venezuelan equine encephalitis (vee) virus rna re…

   Expression

   Mammalian

   Available Since

   Oct. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. T7-phiC31-nos3'UTR

   Plasmid

   #182192

   Purpose

   in vitro transcription of phiC31 integrase mRNA

   Depositor

   David Stern

   Article

   Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

   Insert

   phiC31 integrase
   

   Use

   In vitro transcription

   Available Since

   March 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. T7-CMVtrans-FFLuc-polyA

   Plasmid

   #101156

   Purpose

   Template for T7 RNAP production of RNA transcript for expression of firefly luciferase in a eukaryotic mammalian cell free lysate.

   Depositor

   Marcel Bruchez

   Article

   Canady et al J Am Chem Soc. 2015 Aug 19;137(32):10268

   Insert

   Firefly luciferase
   

   Use

   Luciferase

   Promoter

   T7 promoter

   Available Since

   Jan. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pmtb-t7-alpha-bungarotoxin

   Plasmid

   #69542

   Purpose

   For in vitro transcription of alpha-bungarotoxin mRNA that can be injected into embryos.

   Depositor

   Sean Megason

   Article

   Swinburne et al PLoS One. 2015 Aug 5;10(8):e0134005. doi

   Insert

   alpha-bungarotoxin
   

   Use

   Tol2, beta-actin promoter, sp6,t7 for zebrafish

   Promoter

   beta-actin, sp6, t7

   Available Since

   Nov. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. T7-pAc5.1 mCherry EcoRV

   Plasmid

   #206490

   Purpose

   Schneider cell imaging

   Depositor

   Anne Ephrussi

   Article

   Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

   Type

   Empty backbone

   Tags

   mCherry

   Expression

   Insect

   Available Since

   Sept. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-T7-ABEmax-SpRY

   Plasmid

   #195279

   Purpose

   Allows for in vitro transcription of original pCMV-T7-ABEmax(7.10)-SpRY-P2A-EGFP (RTW5025) without EGFP

   Depositor

   Petros Patsali, Carsten Lederer

   Insert

   human codon optimized ABEmax(7.10) SpCas9 variant named SpRY without EGFP
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSNAP-tag (T7) Vector

   Plasmid

   #101137

   Purpose

   Expression of protein of interest with SNAP-Tag in bacterial cells

   Depositor

   New England Biolabs, Ana Egana

   Type

   Empty backbone

   Tags

   SNAP-tag (SNAP26b)

   Expression

   Bacterial

   Available Since

   Sept. 27, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pBJG1 T7-8xHis-OGT

   Plasmid

   #154271

   Purpose

   Bacterial expression of full length human OGT. N-terminal T7_leader-8xHis tag followed by HRV3C protease site.

   Depositor

   Suzanne Walker

   Article

   Gross et al J Am Chem Soc. 2005 Oct 26;127(42):14588

   Insert

   O-GlcNAc Transferase (OGT Human)
   

   Tags

   8x His, HRV3C protease site, and T7 leader

   Expression

   Bacterial

   Mutation

   bacterial codon optimized

   Available Since

   Sept. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pETDuet-T7-Ct-YhjH

   Plasmid

   #79167

   Purpose

   Dual expression of Ct biosensor and YhjH under individual T7 promoters

   Depositor

   Ming Hammond

   Article

   Wang et al Nucleic Acids Res. 2016 Jul 5. pii: gkw5

   Inserts

   yhjH
   T7-Spinach2-Ct
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEF-BOS-T7-CA150

    Plasmid

    #21924

    Depositor

    Mariano Garcia-Blanco

    Article

    Sune et al Mol Cell Biol. 1999 Jul . 19(7):4719-28.

    Insert

    CA150 (TCERG1 Human)
    

    Tags

    T7

    Expression

    Mammalian

    Available Since

    Sept. 4, 2009

    Availability

    Academic Institutions and Nonprofits only

11. T7-Elongin C-pcDNA3

    Plasmid

    #19998

    Depositor

    William Kaelin

    Article

    Lonergan et al Mol Cell Biol. 1998 Feb . 18(2):732-41.

    Insert

    Elongin C (ELOC Human)
    

    Tags

    T7-tag

    Expression

    Mammalian

    Available Since

    April 6, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. QUAS-0-T7-GFP_ColE1

    Plasmid

    #171649

    Purpose

    QUAS directly upstream of a T7 promoter. Expresses GFP in BL21(DE3) E. coli. Contains ColE1 origin of replication.

    Depositor

    Tara Deans

    Article

    MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

    Insert

    QUAS-0-T7
    

    Tags

    ssrA degradation tag (DAS+4)

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. QUAS-5-T7-GFP_ColE1

    Plasmid

    #171653

    Purpose

    QUAS five base pairs upstream of a T7 promoter. Expresses GFP in BL21(DE3) E. coli. Contains ColE1 origin of replication.

    Depositor

    Tara Deans

    Article

    MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

    Insert

    QUAS-5-T7
    

    Tags

    ssrA degradation tag (DAS+4)

    Expression

    Bacterial

    Available Since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. QUAS-10-T7-GFP_ColE1

    Plasmid

    #171654

    Purpose

    QUAS ten base pairs upstream of a T7 promoter. Expresses GFP in BL21(DE3) E. coli. Contains ColE1 origin of replication.

    Depositor

    Tara Deans

    Article

    MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

    Insert

    QUAS-10-T7
    

    Tags

    ssrA degradation tag (DAS+4)

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. QUAS-15-T7-GFP_ColE1

    Plasmid

    #171655

    Purpose

    QUAS fifteen base pairs upstream of a T7 promoter. Expresses GFP in BL21(DE3) E. coli. Contains ColE1 origin of replication.

    Depositor

    Tara Deans

    Article

    MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

    Insert

    QUAS-15-T7
    

    Tags

    ssrA degradation tag (DAS+4)

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pETM6-T7-VHH-GFPminimizer

    Plasmid

    #202488

    Purpose

    Expresses the nanobody GFP minimizer with T7 induction

    Depositor

    Michael Lynch

    Article

    Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

    Insert

    nanobody GFP minimizer
    

    Tags

    6XHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. 896 pcDNA3 T7 Akt1

    Plasmid

    #9003

    Depositor

    William Sellers

    Article

    Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

    Insert

    Akt1 (AKT1 Human)
    

    Expression

    Mammalian

    Available Since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET30-T7-His-sfGFP

    Plasmid

    #180755

    Purpose

    pET30 vector missing lacO with sfGFP-S-tag inserted between the Bgl II and T7 terminator

    Depositor

    Michael Black

    Article

    Jew et al Front Bioeng Biotechnol. 2022 Jun 23;10:

    Insert

    superfolder green fluorescent protein
    

    Tags

    strep tag

    Expression

    Bacterial

    Available Since

    July 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET47b-T7-AsCas12f-WT

    Plasmid

    #204640

    Purpose

    Expression of wild-type AsCas12f in E. coli

    Depositor

    Weixin Tang

    Article

    Wu et al Nat Chem Biol. 2023 Jul 3. doi: 10.1038/

    Insert

    AsCas12f
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJTM-T7-mNeongreen-6xHis

    Plasmid

    #210868

    Purpose

    Plasmid used for expressing his--tagged mNeongreen for protein purification.

    Depositor

    Richard Murray

    Article

    Meyerowitz et al ACS Synth Biol. 2024 Feb 16;13(2):530-53

    Insert

    mNeongreen-6xHistag
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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