Addgene

We narrowed to 1,426 results for: CAG promoter

Showing: 1181 - 1200 of 1426 results

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Level 1 P4 TaU6 guide acceptor

Plasmid

#165600

Purpose

GoldenGate (MoClo) Level 1 Position 4 TaU6 guide acceptor plasmid

Depositor

John Innes Centre - Wheat Transformation and Genome Editing, Wendy Harwood

Article

Smedley et al Curr Protoc. 2021 Mar;1(3):e65. doi: 10.

Insert

TaU6 promoter::LacZ::sgRNA scaffold

Use

CRISPR and Synthetic Biology; Sgrna acceptor with…

Tags

Expression

Mutation

Promoter

Available since

April 26, 2021

Availability

Academic Institutions and Nonprofits only

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Level 1 P3 TaU6 guide acceptor

Plasmid

#165599

Purpose

GoldenGate (MoClo) Level 1 Position 3 TaU6 guide acceptor plasmid

Depositor

John Innes Centre - Wheat Transformation and Genome Editing, Wendy Harwood

Article

Smedley et al Curr Protoc. 2021 Mar;1(3):e65. doi: 10.

Insert

TaU6 promoter::LacZ::sgRNA scaffold

Use

CRISPR and Synthetic Biology; Sgrna acceptor with…

Tags

Expression

Mutation

Promoter

Available since

April 26, 2021

Availability

Academic Institutions and Nonprofits only

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pTomatoSlicer

Plasmid

#221085

Purpose

Dual-color splicing reporter expressing eGFP and tdTomato from the CMV early enhancer/chicken ß-actin (CAGGS) promoter.

Depositor

Chaolin Zhang

Article

Recinos et al Nat Commun. 2024 May 7;15(1):3839. doi:

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pX330-NEAT1pr_v2

Plasmid

#97082

Purpose

Encodes an sgRNA that creates a DSB at the promoter of human NEAT1 gene

Depositor

Archa Fox

Article

Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

Insert

sgRNA NEAT1pr_v2

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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OA-1050V (wingless)

Plasmid

#132424

Purpose

express arrays of gRNA targeting Wingless under dU6-3 promoter

Depositor

Omar Akbari

Article

Buchman et al CRISPR J. 2020 Jun;3(3):164-176. doi: 10

Insert

wingless gRNA array

Use

Tags

Expression

Insect

Mutation

Promoter

U6-3

Available since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pSM004

Plasmid

#122937

Purpose

Expression of the marked mRNA under the control of substitutional promoters.

Depositor

Attila Becskei

Article

Baudrimont et al Sci Adv. 2017 Jul 12;3(7):e1700006. doi:

Insert

YML065W (Marked)

Use

Tags

Expression

Yeast

Mutation

YML065W: bp 52-63 GAGCAGGGAAAT to GAACAAGGTAAC

Promoter

[tetO2]4inPgal1

Available since

April 9, 2019

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(NANOG_g1)-PGKpuroBFP-W

Plasmid

#211974

Purpose

Express gRNA against NANOG with puro and BFP

Depositor

Kosuke Yusa

Article

Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

Insert

sgRNA targeting NANOG (NANOG Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Human U6 promoter

Available since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pTalpha1-iCre

Plasmid

#133924

Purpose

Neuron-specific expression of iCre

Depositor

Sebastián Dupraz, Frank Bradke

Article

Dupraz et al Curr Biol. 2019 Nov 18;29(22):3874-3886.

Insert

iCre

Use

Tags

Expression

Mammalian

Mutation

Codon optimized

Promoter

neuron-specific tubulin-alpha-1 promoter

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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Lys-eMagB-iRFP

Plasmid

#162249

Purpose

Blue-light dependent heterodimerization system

Depositor

Pietro De Camilli

Article

Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

Insert

p18-eMagB-iRFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

beta-actin promoter

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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eMagB-iRFP-Mito

Plasmid

#162250

Purpose

Blue-light dependent heterodimerization system

Depositor

Pietro De Camilli

Article

Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

Insert

eMagB-iRFP-OMP25

Use

Tags

Expression

Mammalian

Mutation

Promoter

beta-actin promoter

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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eMagA-mCherry-Mito

Plasmid

#162251

Purpose

Blue-light dependent heterodimerization system

Depositor

Pietro De Camilli

Article

Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

Insert

eMagA-mCherry-OMP25

Use

Tags

Expression

Mammalian

Mutation

Promoter

beta-actin promoter

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pR023_PIE-PUM2

Plasmid

#204819

Purpose

Expression PIE-PUM2 fusion protein in mammalian cells

Depositor

Xiaochang Zhang

Article

Ruan et al Nat Commun. 2023 Jun 6;14(1):3275. doi:

Insert

Pumilio 2 (PUM2 Human)

Use

Tags

HA-Flag-rApobec1 and huADAR2dd

Expression

Mammalian

Mutation

Promoter

chicken β-actin promoter

Available since

Nov. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pDEST14-SpyCatcher003-TEVs-SpyTag003DA

Plasmid

#216283

Purpose

Expresses SpyCatcher003-TEV site-SpyTag003DA in bacterial cytoplasm, to allow TEV protease to remove SpyTag003DA and thereby uncage SpyCatcher003 reactivity.

Depositor

Mark Howarth

Article

Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

Insert

SpyCatcher003-TEVs-SpyTag003DA

Use

Tags

His6 and TEV cleavage site

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pX330-GFP-SFPQ

Plasmid

#97084

Purpose

Encodes an sgRNA that creates a DSB at the promoter of human SFPQ gene.

Depositor

Archa Fox

Article

Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

Insert

sgRNA GFP-SFPQ

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

July 31, 2017

Availability

Academic Institutions and Nonprofits only

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pJK03_Rho_basal_DsRed

Plasmid

#173490

Purpose

Vector backbone used to clone MPRA libraries

Depositor

Barak Cohen

Article

White et al Proc Natl Acad Sci U S A. 2013 Jul 16;11

Insert

Bovine Rhodopsin promoter driving DsRed fluorescent protein

Use

Tags

Expression

Mammalian

Mutation

Promoter

Bovine Rhodopsin

Available since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

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Cdk12_intron3_sg2_pX330

Plasmid

#127174

Purpose

sgRNA that cuts within intron 3 of mouse Cdk12 genomic locus in pX330 backbone

Depositor

Phil Sharp

Article

Dubbury et al Nature. 2018 Dec;564(7734):141-145. doi:

Insert

Mouse Cdk12 Intron 3 sgRNA

Use

CRISPR and Mouse Targeting

Tags

Expression

Mutation

Promoter

U6 Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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ER-EGFP-eMagA

Plasmid

#162245

Purpose

Blue-light dependent heterodimerization system

Depositor

Pietro De Camilli

Article

Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

Insert

CytP450 ER targeting sequencel-EGFP-eMagA

Use

Tags

Expression

Mammalian

Mutation

Promoter

beta-actin promoter

Available since

Jan. 5, 2021

Availability

Academic Institutions and Nonprofits only

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ER-mCherry-eMagA

Plasmid

#162248

Purpose

Blue-light dependent heterodimerization system

Depositor

Pietro De Camilli

Article

Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

Insert

CytP450 ER targeting sequencel-mCherry-eMagA

Use

Tags

Expression

Mammalian

Mutation

Promoter

beta-actin promoter

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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TagRFP-T-VAPB(1- 218)- eMagB

Plasmid

#162255

Purpose

Blue-light dependent heterodimerization system

Depositor

Pietro De Camilli

Article

Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

Insert

TagRFP-T-VAPB(1- 218)- eMagB

Use

Tags

Expression

Mammalian

Mutation

Promoter

beta-actin promoter

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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SETMAR-Cas9

Plasmid

#186701

Purpose

Plasmid encoding SETMAR-Cas9 fusion under CMV promoter

Depositor

Jennifer Doudna

Article

Chen et al Proc Natl Acad Sci U S A. 2022 Dec 6;119

Insert

SETMAR-Cas9

Use

CRISPR

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

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