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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,916 results for: FAST

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Showing: 1181 - 1200 of 1916 results

  1. nLuc_AP10_PPVs_P9mS

    Plasmid
    #119302
    Purpose
    Expresses N-terminus of split luciferase fused to antiparallel coiled-coil AP10 connected with autoinhibitory coil P9mS and PPVs cleavage site in antiparallel harpin linker/for SPOC logic
    Depositor
    Roman Jerala
    Publication
    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    split nLuc fused to antiparallel Coiled-coil AP10 connected with P9mS via PPV cleavable linker
    Use
    Luciferase
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    119302-plasmid-map-sequence-id-232037

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  2. pTF-ACE2s

    Plasmid
    #162786
    Purpose
    The extracellular domain of Angiotensin converting enzyme 2 (ACE2) expression
    Depositor
    Ivan Vorobiev
    Publication
    Kolesov et al Diagnostics (Basel). 2022 Feb 3;12(2). p
    Insert
    Angiotensin-converting enzyme 2 (ACE2 Human)
    Tags
    10xHis, FLAG, and hTPA leader
    Expression
    Mammalian
    Promoter
    CHO EEF1A1 (Translation Elongation Factor 1 Alpha…
    Available since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    162786-plasmid-map-sequence-id-333795

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  3. cycLuc_SbMVs

    Plasmid
    #119209
    Purpose
    Circularly permutated firefly luciferase with SbMV cleavage site (Luciferase reporter for detection of proteolytic activity)
    Depositor
    Roman Jerala
    Publication
    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    cyclic luciferase with SbMVp cleavage site
    Use
    Luciferase
    Tags
    AU1 tag
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    119209-plasmid-map-sequence-id-231830

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  4. pCMV-Flag-H3(1-28)-NrdJ-1C-H3.1(10-130)

    Plasmid
    #258599
    Purpose
    Expression of NrdJ-1 C intein inserted in with histone H3.1 in mammalian cells. H3(1-28) is included to increase expression level
    Depositor
    Thomas Muir
    Publication
    Ye et al Chem Sci. 2026 Apr 28. doi: 10.1039/d6sc
    Insert
    Flag-H3(1-28)-NrdJ-1C-H3.1(10-130)
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    includes only aa 1-28 of H3 and aa 10-130 of H3.1
    Promoter
    pCMV
    Available since
    Aug. 17, 2026
    Availability
    Academic Institutions and Nonprofits only
    258599-plasmid-map-sequence-id-518765

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  11. Nabi2.244

    Plasmid
    #73756
    Purpose
    Nabi2.244 is a FRET (Förster Resonance Energy Transfer)-based protein voltage sensor. This probe contains Clover and mRuby2 inserted at different locations in the Ciona voltage sensitive domain.
    Depositor
    Bradley Baker
    Publication
    Sung et al PLoS One. 2015 Nov 20;10(11):e0141585. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Nabi2.244
    Expression
    Mammalian
    Available since
    April 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    73756-plasmid-map-sequence-id-136688

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  12. pcDNA3.1(+)-C-6His-mEsrra

    Plasmid
    #173152
    Purpose
    mouse estrogen-related receptor alpha (Esrra) expression plasmid in mammalian cells
    Depositor
    Brijesh Kumar Singh
    Publication
    Tripathi et al Mol Metab. 2024 Sep;87:101997. doi: 10.1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ORF clone Estrogen-related receptor alpha (Esrra) (Esrra Mouse)
    Tags
    His
    Expression
    Mammalian
    Mutation
    Wild type
    Available since
    Aug. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    173152-plasmid-map-sequence-id-339001

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  14. pET-His-SUMO-NrdJ-1C(N145A)-SUMO

    Plasmid
    #258594
    Purpose
    Expression of His-SUMO tagged NrdJ-1 C intein with an extra SUMO at C-ternimus in bacteria. Originally as splicing deficient mutant for ITC experiment
    Depositor
    Thomas Muir
    Publication
    Ye et al Chem Sci. 2026 Apr 28. doi: 10.1039/d6sc
    Insert
    His-SUMO-NrdJ-1C(N145A)-SUMO
    Tags
    6xHis-SUMO
    Expression
    Bacterial
    Mutation
    N145A, splicing deficient mutation on C intein
    Available since
    Aug. 18, 2026
    Availability
    Academic Institutions and Nonprofits only
    258594-plasmid-map-sequence-id-518777

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Showing: 1181 - 1200 of 1916 results