We narrowed to 15,977 results for: LEA

1. pLenti-CMV-SMCHD1-Exon1-9.41-48-3xFLAG

   Plasmid

   #130685

   Purpose

   Expresses human SMCHD1 Exon1-9.41-48 with a C-terminal 3xFLAG tag

   Depositor

   Stephen Tapscott

   Article

   Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

   Insert

   SMCHD1 Exon1-9.41-48 (SMCHD1 Human)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   I378_Q1684del

   Promoter

   CMV

   Available Since

   Sept. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-CMV-SMCHD1-Exon1-14.47M-48-3xFLAG

   Plasmid

   #130682

   Purpose

   Expresses human SMCHD1 Exon1-14.47M-48 with a C-terminal 3xFLAG tag

   Depositor

   Stephen Tapscott

   Article

   Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

   Insert

   SMCHD1 Exon1-14.47M-48 (SMCHD1 Human)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   E653_G1960del

   Promoter

   CMV

   Available Since

   Sept. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti-CMV-SMCHD1-Exon1-9.47A-48-3xFLAG

   Plasmid

   #130687

   Purpose

   Expresses human SMCHD1 Exon1-9.47A-48 with a C-terminal 3xFLAG tag

   Depositor

   Stephen Tapscott

   Article

   Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

   Insert

   SMCHD1 Exon1-9.47A-48 (SMCHD1 Human)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   I378_E1992del

   Promoter

   CMV

   Available Since

   Sept. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEGFP-RTRG

   Plasmid

   #69845

   Purpose

   The RTRG repeat act as a nuclear/cytoplasmic partition signal in the expressed fusion protein providing increased nuclear to cytoplasmic partition ratio when increasing the number of repeats

   Depositor

   Daniel Christophe

   Article

   Christophe-Hobertus et al Protein Pept Lett. 2008;15(4):397-401.

   Insert

   1 RTRG repeat
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pEGFP-(RTRG)2

   Plasmid

   #69846

   Purpose

   The RTRG repeat act as a nuclear/cytoplasmic partition signal in the expressed fusion protein providing increased nuclear to cytoplasmic partition ratio when increasing the number of repeats

   Depositor

   Daniel Christophe

   Article

   Christophe-Hobertus et al Protein Pept Lett. 2008;15(4):397-401.

   Insert

   2 RTRG repeats
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pEGFP-(RTRG)3

   Plasmid

   #69847

   Purpose

   The RTRG repeat act as a nuclear/cytoplasmic partition signal in the expressed fusion protein providing increased nuclear to cytoplasmic partition ratio when increasing the number of repeats

   Depositor

   Daniel Christophe

   Article

   Christophe-Hobertus et al Protein Pept Lett. 2008;15(4):397-401.

   Insert

   3 RTRG repeats
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pEGFP-(RTRG)4

   Plasmid

   #69848

   Purpose

   The RTRG repeat act as a nuclear/cytoplasmic partition signal in the expressed fusion protein providing increased nuclear to cytoplasmic partition ratio when increasing the number of repeats

   Depositor

   Daniel Christophe

   Article

   Christophe-Hobertus et al Protein Pept Lett. 2008;15(4):397-401.

   Insert

   4 RTRG repeats
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. TALEN-CCR5L18-CtermQ3

   Plasmid

   #51440

   Purpose

   Expresses TALEN targeting left CCR5A site (L18) with Q3 C-terminal domain

   Depositor

   David Liu

   Article

   Guilinger et al Nat Methods. 2014 Apr;11(4):429-35. doi:

   Insert

   TALEN CCR5 Left (L18) Q3 C-term
   

   Use

   TALEN

   Tags

   3xFLAG and Fok1 EL variant

   Expression

   Mammalian

   Mutation

   K788Q, R792Q and R801Q

   Promoter

   CMV

   Available Since

   April 1, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. TALEN-CCR5R18-CtermQ3

   Plasmid

   #51441

   Purpose

   Expresses TALEN targeting right CCR5A site (R18) with Q3 C-terminal domain

   Depositor

   David Liu

   Article

   Guilinger et al Nat Methods. 2014 Apr;11(4):429-35. doi:

   Insert

   TALEN CCR5 Right (R18) Q3 C-term
   

   Use

   TALEN

   Tags

   3xFLAG and Fok1 KK variant

   Expression

   Mammalian

   Mutation

   K788Q, R792Q and R801Q

   Promoter

   CMV

   Available Since

   April 1, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. TALEN-CCR5L18-NtermN1

    Plasmid

    #51444

    Purpose

    Expresses TALEN targeting left CCR5A site (L18) with N1 N-terminal domain

    Depositor

    David Liu

    Article

    Guilinger et al Nat Methods. 2014 Apr;11(4):429-35. doi:

    Insert

    TALEN CCR5 Left (L18) N1 N-term
    

    Use

    TALEN

    Tags

    3xFLAG and Fok1 EL variant

    Expression

    Mammalian

    Mutation

    K150Q

    Promoter

    CMV

    Available Since

    April 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. TALEN-CCR5R18-NtermN1

    Plasmid

    #51445

    Purpose

    Expresses TALEN targeting right CCR5A site (R18) with N1 N-terminal domain

    Depositor

    David Liu

    Article

    Guilinger et al Nat Methods. 2014 Apr;11(4):429-35. doi:

    Insert

    TALEN CCR5 Right (R18) N1 N-term
    

    Use

    TALEN

    Tags

    3xFLAG and Fok1 KK variant

    Expression

    Mammalian

    Mutation

    K150Q

    Promoter

    CMV

    Available Since

    April 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pREP3-TEV-NLS

    Plasmid

    #21146

    Depositor

    Stephen Kearsey

    Article

    Yang et al BMC Mol Biol. 2005 Jun 7;6:13. doi: 10.1

    Insert

    TEV protease (TEVgp1 tobacco etch virus)
    

    Use

    S. pombe

    Tags

    2xNLS, NLS, and myc

    Expression

    Yeast

    Available Since

    July 6, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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13. pOCC305

    Plasmid

    #118898

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for rec. protein with N-terminal TwinStrep-MBP tag, cleavable with 3C and C-terminal HIS6, cleavable with 3C

    Depositor

    PEPC MPI CBG, Marc Bickle

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-TwinStrep-MBP-3C-NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
    

    Tags

    HIS6, cleavable with 3C protease and TwinStrep-MB…

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTXB1-EXO1b

    Plasmid

    #68267

    Purpose

    Bacterial expression of human EXO1

    Depositor

    Stefano Ferrari

    Article

    El-Shemerly et al Cancer Res. 2005 May 1;65(9):3604-9.

    Insert

    EXO1 (EXO1 Human)
    

    Tags

    Mxe intein/chitin binding domain

    Expression

    Bacterial

    Mutation

    b form, codons 1-846. Please see depositor commen…

    Promoter

    T7

    Available Since

    Aug. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

15. Lenti-eCas9-sgNT

    Plasmid

    #140238

    Purpose

    Lentiviral vector expressing high-specificity eSpCas9(1.1) and a control non-targeting sgRNA

    Depositor

    Urs Greber

    Article

    Bauer et al Cell Rep. 2019 Dec 17;29(12):3785-3795.e

    Insert

    non-targeting sgRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFB-LIC-Bse

    Plasmid

    #26108

    Purpose

    SGC Baculovirus transfer vector with His tag in 22-aa N-terminal fusion peptide with TEV protease cleavage site. Includes sites for LIC cloning, and SacB gene for negative selection

    Depositor

    Opher Gileadi

    Article

    Savitsky et al J Struct Biol. 2010 Oct;172(1):3-13. doi

    Type

    Empty backbone

    Use

    Baculovirus expression

    Tags

    His6 - TEV

    Expression

    Insect

    Promoter

    polyhedrin

    Available Since

    Oct. 4, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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17. NLS pEGFP-N3 (1299)

    Plasmid

    #62043

    Purpose

    mammalian expression of NLS-EGFP

    Depositor

    Eric Schirmer

    Article

    Zuleger et al Genome Biol. 2013 Feb 15;14(2):R14. doi:

    Insert

    SV40 NLS
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pALS099 (pGEX-2TP-cTevHis-DEST)

    Plasmid

    #139787

    Purpose

    Gateway destination vector for recombinant protein expression in E. coli; PreScission-cleavable N-terminal GST and TEV-cleavable C-terminal 6xHis

    Depositor

    Arneet Saltzman, Jeannie Lee

    Article

    Saltzman et al Genetics. 2018 Nov;210(3):907-923. doi:

    Type

    Empty backbone

    Tags

    6xHis (cleavable by TEV protease) and GST (cleava…

    Expression

    Bacterial

    Available Since

    April 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. Lenti-eCas9-sgMIB1

    Plasmid

    #140239

    Purpose

    Lentiviral vector expressing high-specificity eSpCas9(1.1) and a gRNA targeting human MIB1

    Depositor

    Urs Greber

    Article

    Bauer et al Cell Rep. 2019 Dec 17;29(12):3785-3795.e

    Insert

    sgRNA targeting human MIB1
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pOCC72

    Plasmid

    #118866

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and N-terminal monomeric GFP, cleavable with TEV

    Depositor

    PEPC MPI CBG, Marc Bickle

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS6-3C-mGFP-TEV-NotI-ccdB-AscI-stop-HindIII cassette
    

    Tags

    HIS6, cleavable with 3C protease, mGFP, cleavable…

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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