We narrowed to 15,783 results for: LEA
-
Plasmid#69848PurposeThe RTRG repeat act as a nuclear/cytoplasmic partition signal in the expressed fusion protein providing increased nuclear to cytoplasmic partition ratio when increasing the number of repeatsDepositorInsert4 RTRG repeats
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pOCC305
Plasmid#118898Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for rec. protein with N-terminal TwinStrep-MBP tag, cleavable with 3C and C-terminal HIS6, cleavable with 3CDepositorInsertNcoI-TwinStrep-MBP-3C-NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
UseTagsHIS6, cleavable with 3C protease and TwinStrep-MB…ExpressionInsectMutationPromoterpolHAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
TALEN-CCR5L18-CtermQ3
Plasmid#51440PurposeExpresses TALEN targeting left CCR5A site (L18) with Q3 C-terminal domainDepositorInsertTALEN CCR5 Left (L18) Q3 C-term
UseTALENTags3xFLAG and Fok1 EL variantExpressionMammalianMutationK788Q, R792Q and R801QPromoterCMVAvailable sinceApril 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
TALEN-CCR5R18-CtermQ3
Plasmid#51441PurposeExpresses TALEN targeting right CCR5A site (R18) with Q3 C-terminal domainDepositorInsertTALEN CCR5 Right (R18) Q3 C-term
UseTALENTags3xFLAG and Fok1 KK variantExpressionMammalianMutationK788Q, R792Q and R801QPromoterCMVAvailable sinceApril 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTXB1-EXO1b
Plasmid#68267PurposeBacterial expression of human EXO1DepositorInsertEXO1 (EXO1 Human)
UseTagsMxe intein/chitin binding domainExpressionBacterialMutationb form, codons 1-846. Please see depositor commen…PromoterT7Available sinceAug. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
Lenti-eCas9-sgNT
Plasmid#140238PurposeLentiviral vector expressing high-specificity eSpCas9(1.1) and a control non-targeting sgRNADepositorInsertnon-targeting sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFB-LIC-Bse
Plasmid#26108PurposeSGC Baculovirus transfer vector with His tag in 22-aa N-terminal fusion peptide with TEV protease cleavage site. Includes sites for LIC cloning, and SacB gene for negative selectionDepositorTypeEmpty backboneUseBaculovirus expressionTagsHis6 - TEVExpressionInsectMutationPromoterpolyhedrinAvailable sinceOct. 4, 2010AvailabilityAcademic Institutions and Nonprofits only -
NLS pEGFP-N3 (1299)
Plasmid#62043Purposemammalian expression of NLS-EGFPDepositorInsertSV40 NLS
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pALS099 (pGEX-2TP-cTevHis-DEST)
Plasmid#139787PurposeGateway destination vector for recombinant protein expression in E. coli; PreScission-cleavable N-terminal GST and TEV-cleavable C-terminal 6xHisDepositorTypeEmpty backboneUseTags6xHis (cleavable by TEV protease) and GST (cleava…ExpressionBacterialMutationPromoterAvailable sinceApril 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
Lenti-eCas9-sgMIB1
Plasmid#140239PurposeLentiviral vector expressing high-specificity eSpCas9(1.1) and a gRNA targeting human MIB1DepositorInsertsgRNA targeting human MIB1
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOCC72
Plasmid#118866Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and N-terminal monomeric GFP, cleavable with TEVDepositorInsertNcoI-HIS6-3C-mGFP-TEV-NotI-ccdB-AscI-stop-HindIII cassette
UseTagsHIS6, cleavable with 3C protease, mGFP, cleavable…ExpressionInsectMutationPromoterpolHAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOCC28
Plasmid#118873Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal MBP tag, cleavable with 3C, and C-terminal HIS6 tag, cleavable with 3CDepositorInsertNcoI-MBP-3C--NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
UseTagsHIS6, cleavable with 3C protease and MBP, cleavab…ExpressionInsectMutationPromoterpolHAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pT3TS-HiFi-nCas9n-A72
Plasmid#125646Purposemicro-injection of poly-adenylated HiFi-nCas9n RNADepositorInsertHiFi-nCas9n
UseRna in vitro transcription vectorTagsExpressionMutationR691A from wild-type Cas9PromoterT3Available sinceJuly 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pALS343 (pGEX-2TP-cTevHis-Gibson/MCS)
Plasmid#139788PurposeVector for recombinant protein expression in E. coli; PreScission-cleavable N-terminal GST and TEV-cleavable C-terminal 6xHisDepositorTypeEmpty backboneUseTags6xHis (cleavable by TEV protease) and GST (cleava…ExpressionBacterialMutationPromoterAvailable sinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pREP3-TEV-NLS
Plasmid#21146DepositorInsertTEV protease (TEVgp1 tobacco etch virus)
UseS. pombeTags2xNLS, NLS, and mycExpressionYeastMutationPromoterAvailable sinceJuly 6, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mCherry-TSC2-NLS
Plasmid#192441PurposeEncodes mCherry-tagged wild-type TSC2 isoform 5 targeted to the nucleus.DepositorInsertmCherry-TSC2-NLS (TSC2 Human)
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailable sinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX330-HA-dSpCas9
Plasmid#92112PurposeExpression plasmid for human codon-optimized dead/inactive SpCas9 (without U6-sgRNA coding sequence)DepositorInsertdead/inactive SpCas9
UseCRISPRTagsHA tag and NLSExpressionMammalianMutationD10A, H840APromoterCbhAvailable sinceOct. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJOE5751.1
Plasmid#135083PurposepBR322-based L-rhamnose-inducible vector.DepositorInsertP-rhaP-BAD-His tag-eGFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGWB21_TaNAM-A1
Plasmid#124572PurposeExpresses full-length TaNAM-A1 with an N-terminal 10x Myc tag under the 35s promoter.DepositorInsertTaNAM-A1
UseTags10xMycExpressionPlantMutationPromoter35sAvailable sinceAug. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOCC193
Plasmid#118903Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal MBP, cleavable with 3C and monomeric GFP and C-terminal TwinStrep, cleavable with 3CDepositorInsertNcoI-MBP-3C-mGFP-NotI-ccdB-AscI-3C-TwinStrep-stop-HindIII cassette
UseTagsMBP, cleavable with 3C protease; mGFP and TwinStr…ExpressionInsectMutationPromoterpolHAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only