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FLAG-MBP1-K295TAG-W340A.pcDNA3-k
Plasmid#126627PurposeExpresses MBP in mammalian cells. Amber codon at AA# 295 as a noncanonical amino acid incorporation site.DepositorInsertMaltose Binding Protein
UseTagsFLAGExpressionMammalianMutationAmino acid mutations: K295 to Amber codon and W34…PromoterCMVAvailable sinceJuly 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Nod-BFP2-TSERex
Plasmid#124785PurposeExpresses the proton pump rhodopsin in mammalian cells under CAG promoterDepositorInsertNod-BFP2
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNP159
Plasmid#106361PurposeTo insert a single copy of a construct at a site on Chr II, corresponding to GFP-labelled RAB-11 expressed specifically in the epidermis.DepositorInsertttTi5605_SEC_dpy-7p_GFP_RAB-11_3'unc-54
UseTagsExpressionWormMutationPromoterAvailable sinceJuly 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
P0050::rpaAD53A
Plasmid#101831Purposedrives expression of rpaA D53A variant from an rpaA-independent promoterDepositorInsertRpaA D53A with p0050 promoter (+ gentamicin resistance casette)
UseCloning vectorTagsExpressionMutationD53 mutated to APromoterAvailable sinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET21a_Msm_RbpA
Plasmid#87928PurposeMycobacterium smegmatis RpbA in pET21a (ampR) used to co-express with Msm SigA for crystallization purposesDepositorInsertRbpA
UseTagsExpressionBacterialMutationPromoterT7PAvailable sinceMarch 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFLIP34
Plasmid#65501PurposeFRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneUseTags6His-AFPt9-attR1 and attR2-t7TFPt9-cMycExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFLIP35
Plasmid#65502PurposeFRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneUseTags6His-AFPt9-attR1 and attR2-TFPt9-cMycExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFLIP36
Plasmid#65504PurposeFRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneUseTags6His-AFPt9-attR1 and attR2-Cer-cMycExpressionMutationPromoterT7 promoterAvailable sinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFLIP42
Plasmid#65617PurposeFRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneUseTags6His-Cit-attR1 and attR2-mCer-cMycExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
p218 pCMV-CREM-L
Plasmid#8396DepositorInsertCREM-L
UseCre/LoxTagsExpressionMammalianMutationmodified Cre, single LOX 511 sitePromoterAvailable sinceApril 28, 2006AvailabilityAcademic Institutions and Nonprofits only -
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C078
Plasmid#174347PurposeTemplate for making mRNA for overexpressing frog ePAB (C-FLAG) in frog oocytesDepositorInsertePAB
UseIn vitro transcription templateTagsFLAGExpressionMutation13 silent mutations: G243A, A273G, G339A, C345G, …PromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
C233
Plasmid#174349PurposeTemplate for making mRNA for overexpressing frog PABPC1 M161A mutant (C-FLAG) in frog oocytesDepositorInsertPABPC1 (M161A) (pabpc1.S Frog)
UseIn vitro transcription templateTagsFLAGExpressionMutationM161APromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_AGIA-AirID
Plasmid#182210PurposeExpress AirID in mammalian cells.DepositorInsertAGIA-AirID
UseTagsAGIA-tagExpressionMammalianMutationPromoterAvailable sinceJuly 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
4xmts-G-Ca-FLITS
Plasmid#191462PurposeGenetically encoded calcium sensor (GECI) G-Ca-FLITS that reports with a lifetime changeDepositorInsertG-Ca-FLITS
UseTags4xmtsExpressionMammalianMutationPromoterAvailable sinceNov. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
p323-L10A-PATagRFP
Plasmid#74172PurposeFluorescent reporter for single ribosome trackingDepositorInsertribosomal protein L10A (Rpl10a Rat)
UseLentiviralTagsPATagRFPExpressionMutationPromoterUbCAvailable sinceApril 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTP264
Plasmid#149334PurposeBacterial expression of Biotin ligase BirADepositorInsertBiotin ligase BirA
UseTagsHis14 and bdNEDD8ExpressionBacterialMutationPromoterAvailable sinceJune 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-tetON-KRAB-dCas9-DHFR-EF1a-TagRFP-2A-tet3G
Plasmid#167935PurposeInducible CRISPRi expressionDepositorInsertKRAB-dCas9-DHFR
UseLentiviralTagsExpressionMutationPromotertetONAvailable sinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits only
