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Addgene
Showing: 1181 - 1200 of 11046 results
  1. pRAN473

    Plasmid
    #120813
    Purpose
    E. coli-C. difficile shuttle vector: tetracycline-inducible promoter (Ptet) driving expression of red fluorescent protein (mCherryOpt), multiple cloning site for fusion to N-terminus of target protein
    Insert
    red fluorescent protein (mCherry), codon-optimized for C. difficile, multiple-cloning site for protein fusion
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Ptet
    Available since
    Feb. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
  2. pSMOX-mNG::LacI-V3

    Plasmid
    #210224
    Purpose
    Integrates a T7 RNA polymerase, tetracycline repressor and an mNeongreen Lac repressor fusion protein with a puromycin drug selection marker into the tubulin locus.
    Depositor
    Insert
    mNeongreen Lac repressor fusion protein
    Use
    Trypanosoma brucei expression
    Tags
    Expression
    Mutation
    Promoter
    Pol II readthrough transcription after integration
    Available since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
  3. MC177VSG221RNAi

    Plasmid
    #59730
    Purpose
    For use in trypanosome cell lines already expressing T7 polymerase and Tet-repressor. Tetracycline induction results in the production of dsRNA targeting VSG221.
    Depositor
    Insert
    VSG221 RNAi fragment
    Use
    RNAi
    Tags
    Expression
    Mutation
    Promoter
    opposing T7 promoters
    Available since
    Aug. 6, 2015
    Availability
    Academic Institutions and Nonprofits only
Showing: 1181 - 1200 of 11046 results