We narrowed to 5,507 results for: SPI

1. pQCXIP-mCherry-CDMPR

   Plasmid

   #182643

   Purpose

   Mammalian expression vector encoding a protein fusion composed of an N-terminal ER targeting sequence of hemagglutinin (HA), followed by mCherry and murine CDMPR (without its signal peptide)

   Depositor

   Martin Spiess

   Article

   Buser et al Life Sci Alliance. 2022 Mar 21;5(7). pii

   Insert

   CDMPR
   

   Use

   Retroviral

   Tags

   N-terminal ER targeting sequence of hemagglutinin…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pQCXIP-EGFP-CDMPR

   Plasmid

   #182642

   Purpose

   Mammalian expression vector encoding a protein fusion composed of an N-terminal ER targeting sequence of hemagglutinin (HA), followed by EGFP and murine CDMPR (without its signal peptide)

   Depositor

   Martin Spiess

   Article

   Buser et al Life Sci Alliance. 2022 Mar 21;5(7). pii

   Insert

   CDMPR
   

   Use

   Retroviral

   Tags

   EGFP and N-terminal ER targeting sequence of hema…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pB_Tet-Off_Flex_DEST_EFS_mODC_tTA (JDW 936)

   Plasmid

   #229828

   Purpose

   A Piggybac compatible, cre dependent, Tet-off destination vector with a downstream WPRE. EFS driving destabilized tTA

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv 2024.07.13.603267

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pME Lamin B Vhh mNeonGreen-pA (JDW 1312)

   Plasmid

   #224501

   Purpose

   Gateway compatible middle entry clone containing Lamin B nanobody fused to mNeonGreen with HA Tag (For visualizing the nuclear lamina, lamin chromobody)

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv 2024.07.13.603267

   Insert

   Lamin B Vhh mNeonGreen-pA
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. p5E-dr-myl7-pro (JDW 1148)

   Plasmid

   #229836

   Purpose

   A gateway compatible 5' entry clone containing the mysoin light chain seven (myl7/cmlc2) promoter for cardiomyocyte specific expression.

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv 2024.07.13.603267

   Insert

   cmlc2/myl7
   

   Use

   Gateway entry clone

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pME-DreERT2 (JDW 1344)

   Plasmid

   #224523

   Purpose

   A Gateway compatible middle entry clone containing Dre recombinase fused to mutant ERT2 for tamoxifen-inducible recombinase activity

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv 2024.07.13.603267

   Insert

   DreERT2
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pT7-7 asyn A53T

   Plasmid

   #105727

   Purpose

   Bacterial expression of mutant human alpha synuclein

   Depositor

   Hilal Lashuel

   Article

   Rospigliosi et al J Mol Biol. 2009 May 22;388(5):1022-32.

   Insert

   alpha-synuclein A53T (SNCA Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   A53T

   Promoter

   T7

   Available since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pME-Sun1-2xsfGFP-6xMYC-pA (JDW 681)

   Plasmid

   #224489

   Purpose

   Gateway compatible middle entry clone containing Sun1 reporter fusion to 2 copies of sfGFP and 6 MYC tags (Nuclear envelope reporter and affinity tag)

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv 2024.07.13.603267

   Insert

   Sun1-2xsfGFP-6xMYC-pA
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pME Actin-Vhh sfGFP (JDW 1215)

   Plasmid

   #224495

   Purpose

   Gateway compatible middle entry clone with Actin nanobody fused to sfGFP (GFP actin reporter)

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv 2024.07.13.603267

   Insert

   Actin-Vhh-sfGFP
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV-CaMKII-jGCaMP7f-WPRE

    Plasmid

    #203848

    Purpose

    Expression of GCaMP7f in CaMKII expressing cells

    Depositor

    Ivo Spiegel

    Article

    Cohen-Kashi Malina et al Neuron. 2021 Jul 7;109(13):2150-2164.e5.

    Insert

    jGCaMP7f
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAdTrack-CMV FLAG Rev-Erb alpha

    Plasmid

    #22745

    Depositor

    Bruce Spiegelman

    Article

    Estall et al Proc Natl Acad Sci U S A. 2009 Dec 29. 1

    Insert

    Rev Erb alpha (NR1D1 Human)
    

    Use

    Adenoviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 6, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA flag PPAR gamma E499Q

    Plasmid

    #8896

    Depositor

    Bruce Spiegelman

    Article

    Hauser et al J Biol Chem. 2000 Jun 16. 275(24):18527-

    Insert

    PPAR gamma (Pparg Mouse)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    E499Q, a mutant in the AF2 domain. Binds PPARg l…

    Promoter

    Available since

    May 24, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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13. pME-3xNLS-mTagBFP2-Cre (JDW 918)

    Plasmid

    #224517

    Purpose

    A Gateway-compatible middle entry clone containing a 3xNLS-mTagBFP2-Cre fusion

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Insert

    3xNLS-mTagBFP2-Cre
    

    Use

    Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-Syn1-fDIO-mScarlet-KASH-2a-dCre

    Plasmid

    #203839

    Purpose

    Flp-dependent enzymatically inactive Cre and mScarlet-KASH expression

    Depositor

    Ivo Spiegel

    Article

    Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

    Insert

    dCre
    

    Use

    AAV

    Tags

    mScarlet-KASH-2A

    Expression

    Mutation

    Enzymatically inactive Cre

    Promoter

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET24a-VHH-mCherry

    Plasmid

    #109421

    Purpose

    Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to mCherry. VHH-mCherry also contains a T7, HA, BAP and His6 epitope

    Depositor

    Martin Spiess

    Article

    Buser et al Proc Natl Acad Sci U S A. 2018 Jul 3;115

    Insert

    anti-GFP nanobody fused to a T7, mCherry, HA, BAP and His6 epitope
    

    Use

    Tags

    BAP, HA, His6, and T7

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA-f:PGC1b

    Plasmid

    #1031

    Depositor

    Bruce Spiegelman

    Article

    Lin et al J Biol Chem 2002 Jan 18;277(3):1645-8. E

    Insert

    PGC-1 beta (Ppargc1b Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 4, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pME Actin Vhh mNeonGreen-HA-pA (JDW 1248)

    Plasmid

    #224500

    Purpose

    Gateway compatible middle entry clone containing Actin nanobody fused to mNeonGreen with an HA tag (Visualizing actin, actin chromobody)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Insert

    Actin-Vhh-mNeonGreen-HA-pA
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pME-EGFP-P2A-iCre (JDW 921)

    Plasmid

    #224519

    Purpose

    A Gateway compatible middle entry clone containing EGFP P2A iCre (codon optimized cre)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Insert

    EGFP-P2A-iCre
    

    Use

    Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. p3E-IRES-3xNLS-mScarlet-I3-pA (JDW 1360)

    Plasmid

    #224536

    Purpose

    A Gateway compatible 3' entry clone containing IRES-FLAG- NLS-mScarlet-I3

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Insert

    IRES-FLAG- NLS-mScarlet-I3
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pME-mTurquoise-P2A-iCre (JDW 920)

    Plasmid

    #224518

    Purpose

    A Gateway compatible middle entry clone containing mTurquoise P2A iCre (codon optimized Cre)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Insert

    mTurquoise-P2A-iCre
    

    Use

    Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge