We narrowed to 5,610 results for: SPI

1. ANKRD24-TwinStrepII

   Plasmid

   #83190

   Purpose

   Expression of tagged protein in mammalian cells

   Depositor

   Peter Barr-Gillespie

   Article

   Morgan et al Elife. 2016 Aug 15;5:e18312. doi: 10.755

   Insert

   ANKRD24 (Ankrd24 Mouse)
   

   Use

   Tags

   Twin Strep Tag II

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA-f:PGC1(delta CTD)

   Plasmid

   #1030

   Depositor

   Bruce Spiegelman

   Article

   Monsalve et al Mol Cell 2000 Aug;6(2):307-16.

   Insert

   PGC1a (Ppargc1a Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   delta CTD

   Promoter

   Available since

   Oct. 4, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET24a-VHH-tev-APEX2

   Plasmid

   #117751

   Purpose

   Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to ascorbate peroxidase 2. VHH-APEX2 also contains a T7, HA, BAP, tev cleavage site and His6 epitope

   Depositor

   Martin Spiess

   Article

   Buser et al J Vis Exp. 2019 Feb 21;(144). doi: 10.37

   Insert

   anti-GFP nanobody fused to a T7, tev site, APEX2, HA, BAP and His6 epitope
   

   Use

   Tags

   BAP, HA, His6, and T7

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. Gal4 PGC1 alpha 1-350

   Plasmid

   #8893

   Depositor

   Bruce Spiegelman

   Article

   Puigserver et al Science 1999 Nov 12;286(5443):1368-71.

   Insert

   PGC-1 alpha (Ppargc1a Mouse)
   

   Use

   Tags

   Gal4 DBD

   Expression

   Mammalian

   Mutation

   amino acids 1-350 of PGC-1 alpha

   Promoter

   Available since

   Aug. 19, 2005

   Availability

   Academic Institutions and Nonprofits only

5. pET24a-LaM4-1xTS

   Plasmid

   #162778

   Purpose

   Bacterial expression of a functionalized anti-mCherry (LaM4) nanobody fused to one tyrosine sulfation (TS) motif. LaM4-1xTS also contains a T7, HA, BAP and His6 epitope

   Depositor

   Martin Spiess

   Article

   Buser et al Life Sci Alliance. 2022 Mar 21;5(7). pii

   Insert

   anti-mCherry nanobody fused to a TS site, T7, HA, BAP and His6 epitope
   

   Use

   Tags

   BAP, HA, His6, T7, and TS site from proCCK

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEX-PGC1(1-180)

   Plasmid

   #1028

   Depositor

   Bruce Spiegelman

   Article

   Puigserver et al Science 1999 Nov 12;286(5443):1368-71.

   Insert

   PGC-1 alpha (Ppargc1a Mouse)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   contains only aa1-180 (the EcoRI site)

   Promoter

   Available since

   Sept. 26, 2005

   Availability

   Academic Institutions and Nonprofits only

7. pB-2x-lns-DEST (JDW 1206)

   Plasmid

   #229818

   Purpose

   A PiggyBac destination vector compatible with gateway with 2 core cHS4 insulators by each ITR site

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. CMV-NF186WT-HA

   Plasmid

   #239774

   Purpose

   Expresses WT rat NF186 with a C-terminal HA tag in mammalian cells.

   Depositor

   Ivana Nikić-Spiegel

   Article

   Stajkovic et al J Cell Sci. 2023 Jun 15;136(12):jcs26060

   Insert

   Nfasc (Nfasc Rat)
   

   Use

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. hNSE-NF186WT-HA

   Plasmid

   #239781

   Purpose

   Expresses WT rat NF186 and with a C-terminal HA tag in mammalian cells.

   Depositor

   Ivana Nikić-Spiegel

   Article

   Stajkovic et al J Cell Sci. 2023 Jun 15;136(12):jcs26060

   Insert

   Nfasc (Nfasc Rat)
   

   Use

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   Promoter

   hNSE (obtained from Addgene plasmid #11606)

   Available since

   July 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pB-Puro-DEST (JDW 925)

    Plasmid

    #229808

    Purpose

    A puromycin selectable, PiggyBac destination vector compatible with 4-way multi-site gateway cloning system

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGLAST-DEST (JDW 1009)

    Plasmid

    #229822

    Purpose

    A single site gateway compatible destination vector with an upstream GLAST promotor to drive expression in glial cells.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pB-Neo-DEST (JDW 940)

    Plasmid

    #229825

    Purpose

    A neo selectable, PiggyBac destination vector compatible with gateway cloning system.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBeta-MHC-promoter DV (JDW 26)

    Plasmid

    #229835

    Purpose

    A gateway compatible destination vector containing the murine beta-MHC promoter for embryonic and postnatal cardiomyocyte expression.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pXMLC2-DEST (JDW 7)

    Plasmid

    #229833

    Purpose

    A gateway compatible destination vector containing the Xenopous myosin light chain 2 promoter for embryonic and adult cardiomyocyte expression.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Type

    Empty backbone

    Use

    Gateway destination vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pME-MCS (JDW 455)

    Plasmid

    #229832

    Purpose

    A gateway compatible middle entry clone containing an MCS (mutiple cloning site).

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    attL1/L2 flanked MCS
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAGEN-DV-IRES-myr-BFP (JDW 491)

    Plasmid

    #229834

    Purpose

    A CAGGS driven gateway compatible destination vector that contains an IRES-myristoylated-TagBFP for labeling the cell membrane

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCS2-phiC31o (JDW 453)

    Plasmid

    #229842

    Purpose

    A CMV-driven Phi31 recombinase.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    phiC31 codon optimized, PhiC31o
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pME-StayGoldc4-Giantin (JDW 1355)

    Plasmid

    #229844

    Purpose

    A gateway compatible middle entry clone containing a V5 tagged n2StayGoldc4 flourescent protein

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    n2StayGoldc4
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. p3E-EF1a-pA (JDW 1222)

    Plasmid

    #224526

    Purpose

    A Gateway compatible 3' entry clone containing rat EF-1a polyA

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    EF-1a polyA
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. p5E_hs_Glast (JDW 1182)

    Plasmid

    #224483

    Purpose

    Gateway compatible 5' entry clone containing the Human GLAST promoter

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    GLAST promoter
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge