We narrowed to 14,071 results for: cas9

1. ER50-SpCas9-ER50

   Plasmid

   #85448

   Purpose

   Expresses SpCas9 fused to ER50 domain on both N- and C-termini in mammalian cells

   Depositor

   Amit Choudhary

   Article

   Maji et al Nat Chem Biol. 2017 Jan;13(1):9-11. doi:

   Insert

   ER50-SpCas9-ER50
   

   Use

   AAV

   Tags

   destabilized domain of estrogen receptor ligand b…

   Expression

   Mammalian

   Promoter

   Cbh

   Available Since

   Feb. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pREDIT_Cas9-MS2-BB_BbsI

   Plasmid

   #164802

   Purpose

   REDIT backbone for wildtype Cas9, pU6-MS2-gRNA-backbone(BbsI)-CBH-SpCas9-T2A-EBFP

   Depositor

   Le Cong

   Article

   Wang et al Nucleic Acids Res. 2021 Apr 6;49(6):e36.

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   Feb. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. SpCas9-CBE6a-IVT

   Plasmid

   #215833

   Purpose

   Template for in vitro transcription of CBE6a (with SpCas9)

   Depositor

   David Liu

   Article

   Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

   Insert

   CBE6a-SpCas9-2xUGI
   

   Use

   In vitro transcription template

   Available Since

   March 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-CMV-SpyCas9

   Plasmid

   #113034

   Purpose

   AAV vector for expression of SpyCas9 in mammalian cells

   Depositor

   Dominik Niopek

   Article

   Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

   Insert

   SpyCas9
   

   Use

   AAV, CRISPR, and Synthetic Biology

   Expression

   Mammalian

   Available Since

   Oct. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPB-R1R2_EF1adCas9VP64_T2A_MS2p65HSF1-IRESbsdpA

   Plasmid

   #112924

   Purpose

   piggyBac transposon vector carrying human EF1a promoter-driven dCas9VP64-T2A-MS2p65HSF1 with Blasticidin resistant marker

   Depositor

   Kosuke Yusa

   Article

   Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

   Insert

   EF1a promoter, dCas9VP94, MS2p65HSF1, IRESneo
   

   Use

   Piggybac transposon vector

   Expression

   Mammalian

   Available Since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. LentiCas9-sgVRK1 #4-Blast

   Plasmid

   #199647

   Purpose

   Expresses Cas9 and sgRNA guide targeting VRK1

   Depositor

   William Hahn

   Article

   So et al JCI Insight. 2022 Oct 10;7(19):e158755.

   Insert

   N/A (VRK1 Human)
   

   Use

   Lentiviral

   Available Since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pYPQ-dpcoCas9-SunTag

   Plasmid

   #158410

   Purpose

   CRISPR-dpcoCas9 fused with SunTag recruiting VP64 activators for transcriptional activation

   Depositor

   Yiping Qi

   Article

   Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

   Insert

   dpcoCas9-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-VP64-GB1-NOS-ter
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   June 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. XLone-Cas9-2A-GFP

   Plasmid

   #170159

   Purpose

   Express Cas9 and GFP upon doxycycline treatment

   Depositor

   Xiaojun Lian

   Article

   Ream et al Stem Cell Reports. 2024 Mar 5:S2213-6711

   Insert

   Cas9
   

   Use

   Synthetic Biology; Piggybac

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   TRE3G promoter

   Available Since

   July 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAN-PTet-dCas9

   Plasmid

   #62244

   Purpose

   controls the expression of S. pyogenes dCas9 from a Tc‐inducible PTet promoter.

   Depositor

   Christopher Voigt

   Article

   Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

   Insert

   dCas9
   

   Use

   CRISPR

   Expression

   Bacterial

   Mutation

   catalytically inactive, D10A, H840A

   Promoter

   Ptet

   Available Since

   July 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-dCas9-D3A

    Plasmid

    #78256

    Purpose

    Expresses dead Cas9 (dCas9) fused to DNMT3A catalytic domain (CD) under the control of the CMV promoter

    Depositor

    Grant Challen

    Article

    McDonald et al Biol Open. 2016 May 11. pii: bio.019067.

    Insert

    DNMT3A CD aa 598-912 (DNMT3A Human)
    

    Use

    CRISPR

    Tags

    Flag epitope tag

    Expression

    Mammalian

    Promoter

    pCMV

    Available Since

    June 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSpCas9(BB)-T2A-Puro_h53BP1_gRNA_D

    Plasmid

    #110302

    Purpose

    Expresssion of Cas9-T2A-puromycin resistant gene and a gRNA targeting exon 10 of human 53BP1

    Depositor

    Chris Kok-Lung Chan

    Article

    Tiwari et al Nat Commun. 2018 Feb 14;9(1):677. doi: 1

    Insert

    p53-binding protein 1 (TP53BP1 Human)
    

    Expression

    Mammalian

    Available Since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. Gephyrin (px330:2-guideCas9)

    Plasmid

    #169432

    Purpose

    Expression of Cas9 and 2 guides

    Depositor

    Richard Huganir

    Article

    Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

    Insert

    spCas9
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    June 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHL-EF1a-SphcCas9-iP-A

    Plasmid

    #60599

    Purpose

    Expresses human codon-optimized Cas9 (derived from Streptococcus pyogenes) and pruomycin resistance gene.

    Depositor

    Akitsu Hotta

    Article

    Li et al Stem Cell Reports. 2014 Nov 25. pii: S22

    Insert

    CRISPR Cas9
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    Codon usage optimized for human usage

    Available Since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. p-dCas9-G9a-Hygro

    Plasmid

    #104408

    Purpose

    transient expression of dCas9-G9a fusion protein

    Depositor

    Stephan Beck

    Insert

    G9a (EHMT2 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV promoter

    Available Since

    April 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMC-J6-dCas9-10XGP41-J9

    Plasmid

    #202005

    Purpose

    MoClo level 0 dCas9-10XGP41 coding sequence module

    Depositor

    Michael Smanski

    Article

    Casas-Mollano et al Nucleic Acids Research, Volume 51, Issue

    Insert

    dCas9-10XGP41
    

    Use

    Synthetic Biology; Moclo cloning vector

    Tags

    GS linkers

    Available Since

    July 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pNS20-SpCas9-SNAP

    Plasmid

    #113717

    Purpose

    Bacterial vector for expression of Snap-tagged Streptococcus pyogenes Cas9

    Depositor

    Gerald Schwank

    Article

    Savic et al Elife. 2018 May 29;7. pii: 33761. doi: 1

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    HA-2xNLS-SNAP-NLS and His6-MBP-TEV

    Expression

    Bacterial

    Available Since

    Sept. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAS9-BFP empty

    Plasmid

    #80976

    Purpose

    Cas9, TagBFP, and sgRNA expression

    Depositor

    Veit Hornung

    Article

    Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. Ezh2[SET]-dCas9

    Plasmid

    #100087

    Purpose

    Catalytic domain [SET] of mouse Ezh2 fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression

    Depositor

    David Segal

    Article

    O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99

    Insert

    Ezh2[SET] (Ezh2 Mouse)
    

    Use

    CRISPR

    Tags

    3XFLag-NLS-Ezh2[SET]-dCas9-NLS

    Expression

    Mammalian

    Mutation

    Ezh2[SET] includes aa 482-746

    Promoter

    CMV

    Available Since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pNOC_dCas9-KRAB_sgRNA Td2

    Plasmid

    #176264

    Purpose

    pNOC episomal plasmid harboring the dead version of humanized spCas9 gene sequence tagged to the KRAB domain and Nlux and sgRNA targeting the fluorescent gene tdtomato with spacer 2

    Depositor

    John van der Oost

    Article

    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

    Insert

    dead spCas9
    

    Use

    CRISPR, Luciferase, and Synthetic Biology ; Expre…

    Tags

    Kruppel associated box domain and luciferase

    Mutation

    H840A and D10A mutations on spCas9 to inactivate …

    Available Since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pREDIT_Cas9n-MS2-BB_BbsI

    Plasmid

    #164804

    Purpose

    REDIT backbone for nickase Cas9n, pU6-MS2-gRNA-backbone(BbsI)-CBH-SpCas9n(D10A)-T2A-EGFP

    Depositor

    Le Cong

    Article

    Wang et al Nucleic Acids Res. 2021 Apr 6;49(6):e36.

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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