We narrowed to 7,593 results for: cre encoding plasmid

1. pSECC

   Plasmid

   #60820

   Purpose

   3rd generation vector. Expresses a sgRNA of interest, Cas9 and Cre

   Depositor

   Tyler Jacks

   Article

   Sanchez-Rivera et al Nature. 2014 Oct 22. doi: 10.1038/nature

   Inserts

   Cas9
   Cre
   

   Use

   CRISPR, Cre/Lox, Lentiviral, and Mouse Targeting

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   BsmBI site eliminated by C->A; E308G

   Promoter

   EFS and EFS (after Cas9-2A)

   Available since

   Nov. 19, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-EF1a-FLEX-GT

   Plasmid

   #26198

   Depositor

   Edward Callaway

   Article

   Wall et al Proc Natl Acad Sci U S A. 2010 Dec 14;10

   Insert

   Enhanced green fluorescent protein, TVA (eGFP TVA is a mouse codon optimized version of an avian gene., Mouse)
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mutation

   TVA mammalian codon optimized

   Promoter

   Available since

   Sept. 16, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-ef1a-DIO-eGCaMP(2+)

   Plasmid

   #201150

   Purpose

   AAV virus production for Cre recombinase dependent expression of eGCaMP(2+)

   Depositor

   Andre Berndt

   Article

   Wait et al Res Sq. 2023 Aug 7:rs.3.rs-3146778. doi:

   Insert

   eGCaMP_2+
   

   Use

   AAV, Cre/Lox, and Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-ef1a-DIO-eGCaMP(plus)

   Plasmid

   #201149

   Purpose

   AAV virus production for Cre recombinase dependent expression of eGCaMP(plus)

   Depositor

   Andre Berndt

   Article

   Wait et al Res Sq. 2023 Aug 7:rs.3.rs-3146778. doi:

   Insert

   eGCaMP+
   

   Use

   AAV, Cre/Lox, and Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJ204-loxP-100RPU.2-ETN-lox2272

   Plasmid

   #154833

   Purpose

   Donor plasmid for Cre/lox-based recombinase-mediated cassette exchange (RMCE), expressing one copy of etanercept

   Depositor

   Lise Marie Grav

   Article

   Sergeeva et al ACS Synth Biol. 2020 Aug 24. doi: 10.102

   Insert

   ETN
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   100RPU.2

   Available since

   Aug. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-Ef1a-DIO SwiChRca-TS-EYFP

   Plasmid

   #55631

   Purpose

   Cre-Dependent Chloride Channel

   Depositor

   Karl Deisseroth

   Article

   Berndt et al Science. 2014 Apr 25;344(6182):420-4. do

   Insert

   iC1C2-TS-EYFP
   

   Use

   AAV and Cre/Lox

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   C128A

   Promoter

   Ef1a

   Available since

   Aug. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-hSyn-frt-DIO-hM3D(Gq)-mCherry

   Plasmid

   #217397

   Purpose

   Expresses the hM3D(Gq) excitatory DREADD fused to mCherry, in a Cre-ON and FLP-OFF dependent manner, under the control of hSyn promoter

   Depositor

   Oscar Marin

   Insert

   hM3D(Gq)-mCherry
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   human Synapsin 1

   Available since

   May 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-hSyn-frt-DIO-hM4D(Gi)-mCherry

   Plasmid

   #217398

   Purpose

   Expresses the hM4D(Gi) inhibitory DREADD fused to mCherry, in a Cre-ON and FLP-OFF dependent manner, under the control of hSyn promoter

   Depositor

   Oscar Marin

   Insert

   hM4D(Gi)-mCherry
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   human Synapsin 1

   Available since

   May 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUCmini-iCAP-PHP.V1

   Plasmid

   #127847

   Purpose

   non-standard AAV2 rep-AAV-PHP.V1 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

   Depositor

   Viviana Gradinaru

   Article

   Ravindra Kumar et al Nat Methods. 2020 May;17(5):541-550. doi

   Insert

   Synthetic construct isolate AAV-PHP.V1 VP1 gene
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   p41

   Available since

   April 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUCmini-iCAP-PHP.B

    Plasmid

    #103002

    Purpose

    non-standard AAV2 rep-AAV-PHP.B cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

    Depositor

    Viviana Gradinaru

    Article

    Deverman et al Nat Biotechnol. 2016 Feb;34(2):204-9. do

    Insert

    Synthetic construct isolate AAV-PHP.B VP1 gene
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    p41

    Available since

    Feb. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUCmini-iCAP-PHP.N

    Plasmid

    #127851

    Purpose

    non-standard AAV2 rep-AAV-PHP.N cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

    Depositor

    Viviana Gradinaru

    Article

    Ravindra Kumar et al Nat Methods. 2020 May;17(5):541-550. doi

    Insert

    Synthetic construct isolate AAV-PHP.N VP1 gene
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    p41

    Available since

    April 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUCmini-iCAP-PHP.B3

    Plasmid

    #103004

    Purpose

    non-standard AAV2 rep-AAV-PHP.B3 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

    Depositor

    Viviana Gradinaru

    Article

    Deverman et al Nat Biotechnol. 2016 Feb;34(2):204-9. do

    Insert

    Synthetic construct isolate AAV-PHP.B3 VP1 gene
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    p41

    Available since

    Feb. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUCmini-iCAP-PHP.B2

    Plasmid

    #103003

    Purpose

    non-standard AAV2 rep-AAV-PHP.B2 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

    Depositor

    Viviana Gradinaru

    Article

    Deverman et al Nat Biotechnol. 2016 Feb;34(2):204-9. do

    Insert

    Synthetic construct isolate AAV-PHP.B2 VP1 gene
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    p41

    Available since

    Dec. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUCmini-iCAP-PHP.V2

    Plasmid

    #127848

    Purpose

    non-standard AAV2 rep-AAV-PHP.V2 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

    Depositor

    Viviana Gradinaru

    Article

    Ravindra Kumar et al Nat Methods. 2020 May;17(5):541-550. doi

    Insert

    Synthetic construct isolate AAV-PHP.V2 VP1 gene
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    p41

    Available since

    April 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUCmini-iCAP-PHP.B4

    Plasmid

    #127849

    Purpose

    non-standard AAV2 rep-AAV-PHP.B4 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

    Depositor

    Viviana Gradinaru

    Article

    Ravindra Kumar et al Nat Methods. 2020 May;17(5):541-550. doi

    Insert

    Synthetic construct isolate AAV-PHP.B4 VP1 gene
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    p41

    Available since

    April 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV.hSyn.FLEX.SYFP2-POST-T2A-dTomato.FLEX.WPRE.SV40

    Plasmid

    #105983

    Purpose

    This AAV plasmid in the presence of Cre recombinase expresses an extracellular SYFP2 fused to the neuroligin-1 cytoplasmic domain for targeting to the synapse with a cell fill dTomato.

    Depositor

    Marcel Bruchez

    Article

    Kuljis et al eNeuro. 2019 Sep 23. pii: ENEURO.0193-19

    Insert

    FLEX-SYFP2-POST-T2A-dTomato.FLEX
    

    Use

    AAV and Cre/Lox

    Tags

    SYFP2-POST, T2A-dTomato, and myc

    Expression

    Mammalian

    Mutation

    Promoter

    human Synapsin 1

    Available since

    Oct. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV.hSyn.FLEX.FAPdL5-POST-T2A-dTomato.FLEX.WPRE.SV40

    Plasmid

    #105982

    Purpose

    This AAV plasmid in the presence of Cre recombinase expresses an extracellular dL5 FAP fused to the neuroligin-1 cytoplasmic domain for targeting to the synapse with a cell fill dTomato.

    Depositor

    Marcel Bruchez

    Article

    Kuljis et al eNeuro. 2019 Sep 23. pii: ENEURO.0193-19

    Insert

    FLEX-FAPdL5-POST-T2A-dTomato.FLEX
    

    Use

    AAV and Cre/Lox

    Tags

    FAPdL5-POST, T2A-dTomato, and myc

    Expression

    Mammalian

    Mutation

    Promoter

    human synapsin 1

    Available since

    Oct. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-hSyn-FLEX-TVA-P2A-EGFP-2A-oG

    Plasmid

    #85225

    Purpose

    Expresses TVA, EGFP, and optimized Rabies G (oG) protein in a FLEX cassette

    Depositor

    John Naughton

    Inserts

    Optimized G Protein
    Enhanced GFP
    TVA (CD320 Chicken)
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    Jan. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLP-hSox2-ires-gfp

    Plasmid

    #49391

    Purpose

    mammalian bicistronic expression of human Sox2 and EGFP

    Depositor

    Randall McKinnon

    Insert

    Sox2 (SOX2 Human)
    

    Use

    Cre/Lox; Acceptor vector

    Tags

    6xHN, EGFP, and IRES

    Expression

    Mammalian

    Mutation

    TGA (stop) to CCA (Pro) at nt 1027 with respect t…

    Promoter

    CMV

    Available since

    Dec. 2, 2013

    Availability

    Academic Institutions and Nonprofits only

20. pAAV-EF1a-Con/Fon-GCaMP6M

    Plasmid

    #137119

    Purpose

    Intersectional viral expression of GCaMP6M in cells expressing both Cre AND Flp

    Depositor

    Karl Deisseroth, INTRSECT 2.0 Project

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV8

    Insert

    Con/Fon-GCaMP6M
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Tags

    Expression

    Mutation

    Removed RSET tag

    Promoter

    EF1a

    Available since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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