168,238 results

1. pCDNA3.1-FLAG-hSF3B1-K700E

   Plasmid

   #82577

   Purpose

   Expresses a codon-optimized ORF of human SF3B1 ( K700E mutant)

   Depositor

   Manoj Pillai

   Article

   Kesarwani et al Oncogene. 2016 Aug 15. doi: 10.1038/onc.

   Insert

   human SF3B1-K700E (SF3B1 Human)
   

   Tags

   FLAG peptide

   Expression

   Mammalian

   Mutation

   K700E

   Promoter

   CMV

   Available Since

   March 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDAC565

   Plasmid

   #195242

   Purpose

   Mammalian expression of Csm-GFP complex (RNase mut) from separate promoters (Used for RNA imaging)

   Depositor

   Jennifer Doudna

   Article

   Colognori et al Nat Biotechnol. 2023 Jan 23. doi: 10.103

   Insert

   FLAG-NLS-Csm1; FLAG-NLS-Csm2, FLAG-NLS-Csm3(RNase mut)-GFP; FLAG-NLS-Csm4; FLAG-NLS-Csm5; FLAG-NLS-Cas6; crRNA
   

   Expression

   Mammalian

   Available Since

   Jan. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. GST-RH▲-MNase

   Plasmid

   #136292

   Purpose

   Expression of catalytic inactive RNase H fused to micrococcal nuclease

   Depositor

   Kavitha Sarma

   Article

   Yan et al Cell Rep. 2019 Oct 29;29(5):1369-1380.e5

   Insert

   RNase HI-D10R-E48R and MNase
   

   Expression

   Bacterial

   Available Since

   Feb. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEGFP-C1X-Nup88

   Plasmid

   #87338

   Purpose

   To express human Nup88 in mammalian cells

   Depositor

   Lei Lu

   Article

   Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

   Insert

   NUP88 (NUP88 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. p413 TEF NAPstarC

   Plasmid

   #232737

   Purpose

   Expresses control sensor NAPstarC in S. cerevisiae.

   Depositor

   Bruce Morgan

   Article

   Scherschel et al Nat Commun. 2024 Dec 19;15(1):10704. doi

   Insert

   NAPstarC
   

   Expression

   Yeast

   Available Since

   April 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pB-CAGGS-dCas9-KRAB-MeCP2

   Plasmid

   #110824

   Purpose

   PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2

   Depositor

   Alejandro Chavez, George Church

   Article

   Yeo et al Nat Methods. 2018 Jul 16. pii: 10.1038/s

   Insert

   dCas9-KRAB-MeCP2
   

   Expression

   Mammalian

   Mutation

   Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

   Promoter

   CAGGS

   Available Since

   July 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHR_PGK_antiHer24D5-7_synNotch_Gal4VP64

   Plasmid

   #85424

   Purpose

   Lentiviral vector for expression of anti-Her2 scFv 24D5-7 Gal4VP64 synthetic Notch

   Depositor

   Wendell Lim

   Article

   Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

   Insert

   anti-Her2_4D5-7_synNotch
   

   Use

   Lentiviral

   Promoter

   PGK

   Available Since

   Sept. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. loxP-Puro-loxP-3xV5-SNAP-Rab11 HR

   Plasmid

   #229677

   Purpose

   Homology repair plasmid for endogenous tagging of Rab11 at the N-terminus with SNAP tag and a 3x V5 epitope tag. Contains a puromycin resistance cassette for selection of edited cells.

   Depositor

   Francesca Bottanelli

   Article

   Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/

   Insert

   genomic sequence Rab11 locus (RAB11A Human)
   

   Use

   CRISPR

   Tags

   SNAP, 3xV5

   Available Since

   Jan. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. lenti-mito-mTagBFP2

   Plasmid

   #216130

   Purpose

   Lentiviral vector for expressing mTagBFP2 localizing to mitochondria.

   Depositor

   Stefan Kubicek

   Article

   Reicher et al Nat Cell Biol. 2024 May;26(5):745-756. d

   Insert

   EF1a-COX8AmitochondrialLocalizationSignal-mTagBFP2-WPRE
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLVX-ATF4 mScarlet NLS

    Plasmid

    #115969

    Purpose

    ER-stress sensor - ATF4 translation at ORF3 fluorescent reporter with mScarlet-I

    Depositor

    David Andrews

    Article

    Nougarede et al Assay Drug Dev Technol. 2018 Aug 8. doi:

    Insert

    activating transcription factor 4 (ATF4 Synthetic, Human)
    

    Use

    Lentiviral

    Tags

    HA tag, c-myc NLS, and mScarlet-I

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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