Skip to main content
Addgene
Showing: 2401 - 2440 of 57997 results

  1. mPlum-PCNA-19-SV40NLS-4

    Plasmid
    #55994
    Purpose
    Localization: Nucleus, Excitation: 590, Emission: 649
    Depositor
    Michael Davidson
    Insert
    PCNA-19 SV40NLS (PCNA Human)
    Use
    Tags
    mPlum
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only

  2. R777-E274 Hs.RCE1-nostop

    Plasmid
    #70558
    Purpose
    Gateway ORF clone of human RCE1 [NM_005133.2] without stop codon (for C-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    RCE1 (RCE1 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70558-plasmid-map-sequence-id-126007

    Enlarge

  3. R777-E189 Hs.PREX2

    Plasmid
    #70473
    Purpose
    Gateway ORF clone of human PREX2 [NM_024870.2] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    PREX2 (PREX2 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70473-plasmid-map-sequence-id-125922

    Enlarge

  4. R777-E295 Hs.RPS6KA6

    Plasmid
    #70579
    Purpose
    Gateway ORF clone of human RPS6KA6 [NM_014496.4] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    RPS6KA6 (RPS6KA6 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70579-plasmid-map-sequence-id-126028

    Enlarge

  5. R777-E289 Hs.RPS6KA1

    Plasmid
    #70573
    Purpose
    Gateway ORF clone of human RPS6KA1 [NM_002953.3] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    RPS6KA1 (RPS6KA1 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70573-plasmid-map-sequence-id-181742

    Enlarge

  6. R777-E186 Hs.PLXNB1-nostop

    Plasmid
    #70470
    Purpose
    Gateway ORF clone of human PLXNB1 [NM_001130082.2] without stop codon (for C-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    PLXNB1 (PLXNB1 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70470-plasmid-map-sequence-id-125919

    Enlarge

  7. pFETCh_TGIF2

    Plasmid
    #72373
    Purpose
    Donor vector for 3' FLAG tag of human TGIF2
    Depositor
    Eric Mendenhall, Richard M. Myers
    Insert
    TGIF2 Homology Arms (TGIF2 Human)
    Use
    CRISPR
    Tags
    3XFLAG-P2A-NeoR
    Expression
    Mutation
    Promoter
    Available since
    Feb. 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    72373-plasmid-map-sequence-id-132123

    Enlarge

  8. mApple-Zyxin-6

    Plasmid
    #54964
    Purpose
    Localization: Focal Adhesions, Excitation: 568, Emission: 592
    Depositor
    Michael Davidson
    Insert
    Zyxin (ZYX Human)
    Use
    Tags
    mApple
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 7, 2014
    Availability
    Academic Institutions and Nonprofits only

  9. MIGR1-PREP1

    Plasmid
    #78147
    Purpose
    retroviral expression of PREP1 and GFP
    Depositor
    Francesco Blasi
    Insert
    PREP1 (PKNOX1 Human)
    Use
    Retroviral
    Tags
    IRES-GFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 10, 2016
    Availability
    Academic Institutions and Nonprofits only

  10. pHH0103 SMURF2 WW domain #3

    Plasmid
    #104226
    Purpose
    Bacterial expression of WW domain #3 from SMURF2
    Depositor
    Marius Sudol, Sachdev Sidhu
    Insert
    SMURF2 WW domain #3 (SMURF2 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available since
    Feb. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    104226-plasmid-map-sequence-id-204295

    Enlarge

  11. pHH0103 SMURF2 WW domain #2

    Plasmid
    #104225
    Purpose
    Bacterial expression of WW domain #2 from SMURF2
    Depositor
    Marius Sudol, Sachdev Sidhu
    Insert
    SMURF2 WW domain #2 (SMURF2 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available since
    Feb. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    104225-plasmid-map-sequence-id-204192

    Enlarge

  12. pHH0103 SMURF2 WW domain #1

    Plasmid
    #104224
    Purpose
    Bacterial expression of WW domain #1 from SMURF2
    Depositor
    Marius Sudol, Sachdev Sidhu
    Insert
    SMURF2 WW domain #1 (SMURF2 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available since
    Jan. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    104224-plasmid-map-sequence-id-201513

    Enlarge

  13. pFETCh_ZNF146

    Plasmid
    #72359
    Purpose
    Donor vector for 3' FLAG tag of human ZNF146
    Depositor
    Eric Mendenhall, Richard M. Myers
    Insert
    ZNF146 Homology Arms (ZNF146 Human)
    Use
    CRISPR
    Tags
    3XFLAG-P2A-NeoR
    Expression
    Mutation
    Promoter
    Available since
    Feb. 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    72359-plasmid-map-sequence-id-135361

    Enlarge

  14. Dronpa-Zyxin-6

    Plasmid
    #57309
    Purpose
    Localization: Focal Adhesions, Excitation: 503, Emission: 518
    Depositor
    Michael Davidson
    Insert
    Zyxin (ZYX Human)
    Use
    Tags
    Dronpa
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 13, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  15. R777-E191 Hs.PRKAA1

    Plasmid
    #70475
    Purpose
    Gateway ORF clone of human PRKAA1 [NM_006251.5] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    PRKAA1 (PRKAA1 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70475-plasmid-map-sequence-id-125924

    Enlarge

  16. Nup133_mEGFP(-9a)

    Plasmid
    #163419
    Purpose
    Mammalian expression of Nup133 fused at its carboxyl-terminus to mEGFP with no linker and a total net fusion of (-9 amino acids)
    Depositor
    Sanford Simon
    Insert
    Nup133 (NUP133 Human)
    Use
    Tags
    mEGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    163419-plasmid-map-sequence-id-321638

    Enlarge

  17. mApple-PCNA-19-NLS-4

    Plasmid
    #54937
    Purpose
    Localization: Nucleus, Excitation: 568, Emission: 592
    Depositor
    Michael Davidson
    Insert
    PCNA-19 NLS (PCNA Human)
    Use
    Tags
    mApple
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 7, 2014
    Availability
    Academic Institutions and Nonprofits only

  18. R777-E133 Hs.MLST8

    Plasmid
    #70417
    Purpose
    Gateway ORF clone of human MLST8 [NM_022372.4] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    MLST8 (MLST8 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70417-plasmid-map-sequence-id-125866

    Enlarge

  19. TARS_pNIC-Bio3

    Plasmid
    #153057
    Purpose
    Expression construct for producing Avi tagged Threonyl-tRNA synthetase
    Depositor
    Susanne Gräslund
    Insert
    TARS (TARS1 Human)
    Use
    Tags
    Avi and His6-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    June 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    153057-plasmid-map-sequence-id-304477

    Enlarge

  20. pE-SUMO CRYGB

    Plasmid
    #80751
    Purpose
    Expression of human gamma B-crystallin in E. coli
    Depositor
    Larry David
    Insert
    CRYGB (CRYGB Human)
    Use
    Tags
    6xHIS and Smt3 SUMO
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    80751-plasmid-map-sequence-id-153519

    Enlarge

  21. R777-E201 Hs.PRKAG2

    Plasmid
    #70485
    Purpose
    Gateway ORF clone of human PRKAG2 [NM_024429.1] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    PRKAG2 (PRKAG2 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70485-plasmid-map-sequence-id-125934

    Enlarge

  22. R777-E251 Hs.RASSF1

    Plasmid
    #70535
    Purpose
    Gateway ORF clone of human RASSF1 [NM_007182.4] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    RASSF1 (RASSF1 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70535-plasmid-map-sequence-id-125984

    Enlarge

  23. MTMR6A

    Plasmid
    #42483
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    MTMR6A
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    See Comments
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  24. mApple-Moesin-C-14

    Plasmid
    #54927
    Purpose
    Localization: Cytoskeleton/Membrane, Excitation: 568, Emission: 592
    Depositor
    Michael Davidson
    Insert
    Moesin (MSN Human)
    Use
    Tags
    mApple
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 7, 2014
    Availability
    Academic Institutions and Nonprofits only

  25. mVenus-Zyxin-6

    Plasmid
    #56626
    Purpose
    Localization: Focal Adhesions, Excitation: 515, Emission: 528
    Depositor
    Michael Davidson
    Insert
    Zyxin (ZYX Human)
    Use
    Tags
    mVenus
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 18, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  26. R777-E196 Hs.PRKAB1-nostop

    Plasmid
    #70480
    Purpose
    Gateway ORF clone of human PRKAB1 [NM_006253.4] without stop codon (for C-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    PRKAB1 (PRKAB1 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70480-plasmid-map-sequence-id-125929

    Enlarge

  27. INPP5EA

    Plasmid
    #42481
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    INPP5EA
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    See Comments
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  28. R777-E266 Hs.RASSF7-nostop

    Plasmid
    #70550
    Purpose
    Gateway ORF clone of human RASSF7 [NM_001143993.1] without stop codon (for C-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    RASSF7 (RASSF7 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70550-plasmid-map-sequence-id-358342

    Enlarge

  29. R777-E304 Hs.SAV1-nostop

    Plasmid
    #70588
    Purpose
    Gateway ORF clone of human SAV1 [NM_021818.3] without stop codon (for C-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    SAV1 (SAV1 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70588-plasmid-map-sequence-id-126037

    Enlarge

  30. R777-E188 Hs.PPP1CA-nostop

    Plasmid
    #70472
    Purpose
    Gateway ORF clone of human PPP1CA [NM_002708.3] without stop codon (for C-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    PPP1CA (PPP1CA Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70472-plasmid-map-sequence-id-125921

    Enlarge

  31. pCB94

    Plasmid
    #69743
    Purpose
    Retroviral expression of PP2A beta
    Depositor
    Iain Cheeseman
    Insert
    PP2A beta (PTPA Human)
    Use
    Retroviral
    Tags
    eGFP-TEV-S
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 3, 2015
    Availability
    Academic Institutions and Nonprofits only

  32. 4I79

    Plasmid
    #51319
    Depositor
    Cheryl Arrowsmith
    Insert
    4I79 (NUP43 Human)
    Use
    Baculovirus expression
    Tags
    His
    Expression
    Mutation
    Truncation: See Comments
    Promoter
    T7
    Available since
    Feb. 24, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  33. mKO2-PCNA-19-SV40NLS-4

    Plasmid
    #57890
    Purpose
    Localization: Nucleus, Excitation: 551, Emission: 565
    Depositor
    Michael Davidson
    Insert
    PCNA-19 SV40NLS (PCNA Human)
    Use
    Tags
    mKO2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 24, 2014
    Availability
    Academic Institutions and Nonprofits only
    57890-plasmid-map-sequence-id-233994

    Enlarge

  34. pHH0103 WBP4 WW domain #2

    Plasmid
    #104253
    Purpose
    Bacterial expression of WW domain #2 from WBP4
    Depositor
    Marius Sudol, Sachdev Sidhu
    Insert
    WBP4 WW domain #2 (WBP4 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available since
    Feb. 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    104253-plasmid-map-sequence-id-204383

    Enlarge

  35. pE-SUMO CRYGC

    Plasmid
    #80754
    Purpose
    Expression of human gamma C-crystallin in E. coli
    Depositor
    Larry David
    Insert
    CRYGC (CRYGC Human)
    Use
    Tags
    6xHIS and Smt3 SUMO
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    80754-plasmid-map-sequence-id-153567

    Enlarge

  36. R777-E232 Hs.RASA3-nostop

    Plasmid
    #70516
    Purpose
    Gateway ORF clone of human RASA3 [NM_007368.2] without stop codon (for C-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    RASA3 (RASA3 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70516-plasmid-map-sequence-id-125965

    Enlarge

  37. R777-E309 Hs.SHC2

    Plasmid
    #70593
    Purpose
    Gateway ORF clone of codon optimized human SHC2 [NM_012435.2] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    SHC2 (SHC2 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70593-plasmid-map-sequence-id-126042

    Enlarge

  38. tdEos-PLK1-N-16

    Plasmid
    #57657
    Purpose
    Localization: Centrosomes, Excitation: 505 / 569, Emission: 516 / 581
    Depositor
    Michael Davidson
    Insert
    PLK1 (PLK1 Human)
    Use
    Tags
    tdEos
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 5, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  39. tdEos-ZO1-C-14

    Plasmid
    #57694
    Purpose
    Localization: Tight Junctions, Excitation: 505 / 569, Emission: 516 / 581
    Depositor
    Michael Davidson
    Insert
    ZO1 (TJP1 Human)
    Use
    Tags
    tdEos
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 13, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  40. mPlum-Zyxin-6

    Plasmid
    #56009
    Purpose
    Localization: Focal Adhesions, Excitation: 590, Emission: 649
    Depositor
    Michael Davidson
    Insert
    Zyxin (ZYX Human)
    Use
    Tags
    mPlum
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
Showing: 2401 - 2440 of 57997 results