We narrowed to 3,649 results for: WOL

1. pEJS887_pTetR-P2A-BFPnls/sgAlpha

   Plasmid

   #108651

   Purpose

   Alpha satellite repeats targeting Spy sgRNA under U6 promoter

   Depositor

   Erik Sontheimer

   Article

   Gao et al Nat Methods. 2018 Jun;15(6):433-436. doi

   Inserts

   Alpha satellite repeats targeting sgRNA
   TetR-P2A-BFP
   

   Use

   CRISPR and Lentiviral

   Tags

   No

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and hPGK

   Available since

   May 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1 CARD8-TEV-GFP-FLAG

   Plasmid

   #169991

   Purpose

   Mammalian purification construct for CARD8

   Depositor

   Daniel Bachovchin

   Article

   Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

   Insert

   CARD8 (CARD8 Human)
   

   Use

   Tags

   TEV-GFP-FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pIA1385

   Plasmid

   #166862

   Purpose

   Codon optimized SARS-CoV-2 Nsp12 gene was cloned into a modified pRSFDuet-1 vector (Addgene #159107) for expression in E. coli.

   Depositor

   Irina Artsimovitch

   Article

   Wang et al mBio. 2021 Jun 29;12(3):e0142321. doi: 1

   Insert

   SARS-COV-2 Nsp12 (ORF1ab Synthetic)
   

   Use

   Tags

   His-SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRK5-FLAG-Sestrin1 isoform1

   Plasmid

   #72594

   Purpose

   overexpression, codon optimized for mammalian expression

   Depositor

   David Sabatini

   Article

   Chantranupong et al Cell Rep. 2014 Oct 9;9(1):1-8. doi: 10.1

   Insert

   SESN1 (SESN1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   codon optimized for mammalian expression, see dep…

   Promoter

   CMV

   Available since

   Feb. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

5. E42_gRunx1_gRNA2_dTet_NGFR

   Plasmid

   #189801

   Purpose

   Retroviral delivery of guide RNA against mouse Runx1

   Depositor

   Ellen Rothenberg

   Article

   Shin et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

   Insert

   gRunx1_gRNA2 (Runx1 Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. E42_gRunx1_gRNA1_dTet_NGFR

   Plasmid

   #189802

   Purpose

   Retroviral delivery of guide RNA against mouse Runx1

   Depositor

   Ellen Rothenberg

   Article

   Shin et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

   Insert

   gRunx1_gRNA1 (Runx1 Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. MSCV-human NMyc-humanER-IRES-mCherry

   Plasmid

   #73571

   Purpose

   Mammalian retroviral expression of NMyc-ER

   Depositor

   Martine Roussel

   Article

   Vo et al Cancer Cell. 2016 Jan 11;29(1):5-16. doi

   Insert

   NMyc (MYCN Human)
   

   Use

   Retroviral

   Tags

   Human ER and IRES-mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   MSCV

   Available since

   Feb. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

8. pCW57.1_V5_mNeonGreen_FLAG_C

   Plasmid

   #218073

   Purpose

   RESOLUTE cloning plasmid

   Depositor

   Giulio Superti-Furga, RESOLUTE Consortium

   Article

   Gelová et al J Mol Biol. 2024 Jun 13:168665. doi: 10.

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   FLAG-mNeonGreen-V5

   Expression

   Mutation

   Promoter

   Available since

   May 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRK5-FLAG-Sestrin3

   Plasmid

   #72592

   Purpose

   overexpression, codon optimized for mammalian expression

   Depositor

   David Sabatini

   Article

   Chantranupong et al Cell Rep. 2014 Oct 9;9(1):1-8. doi: 10.1

   Insert

   SESN3 (SESN3 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   codon optimized for mammalian expression, see dep…

   Promoter

   CMV

   Available since

   March 28, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pCS2 Smad1 EVE GM

    Plasmid

    #27997

    Depositor

    Edward De Robertis

    Article

    Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

    Insert

    Human Smad1 EVE Gsk3 mutant (SMAD1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    S463E S465E S210A T202A S191A S198A

    Promoter

    Available since

    Sept. 12, 2011

    Availability

    Academic Institutions and Nonprofits only

11. pBS-f1(-)-ACTB-mEGFP

    Plasmid

    #115917

    Purpose

    donor to insert EGFP at the ACTB locus in human cells

    Depositor

    Erik Sontheimer

    Article

    Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

    Insert

    ACTB-mEGFP
    

    Use

    Donor template

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    Available since

    March 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHDR_NANOS3-T2A-mVenus-PuroTK

    Plasmid

    #222903

    Purpose

    Homology directed repair template for knocking in mVenus reporter to NANOS3.

    Depositor

    George Church

    Article

    Pierson Smela et al bioRxiv 2022.07.11.499564

    Insert

    mVenus
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGC(2.1)-U6.3

    Plasmid

    #170516

    Purpose

    PCR template vector for amplifying gRNAcore(2.1)-pU6.3 promoter fragment; used together with pAC-CR7T-gRNA2.1-nlsBFP

    Depositor

    Chun Han

    Article

    Koreman et al Proc Natl Acad Sci U S A. 2021 Apr 6;118

    Insert

    gRNAcore(2.1)-pU6.3
    

    Use

    Pcr template vector for amplifying grnacore(2.1)-…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBS-f1(-)-GAPDH-IRES-EGFP

    Plasmid

    #115918

    Purpose

    donor to insert IRES-EGFP at the GAPDH locus in human cells

    Depositor

    Erik Sontheimer

    Article

    Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

    Insert

    IRES-EGFP
    

    Use

    Donor template

    Tags

    dTomato

    Expression

    Mutation

    Promoter

    Available since

    March 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. YIp128-CUP1-NUbo-GFP-His6

    Plasmid

    #212790

    Purpose

    Copper-inducible expression of the artificial test substrate NUbo-GFP-His6 in budding yeast

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    NUbo-GFP-His6
    

    Use

    Integrative vector

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    CUP1

    Available since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBS-f1(-)-TOMM20-mEGFP

    Plasmid

    #115923

    Purpose

    donor to insert EGFP at the TOMM20 locus in human cells

    Depositor

    Erik Sontheimer

    Article

    Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

    Insert

    TOMM20-mEGFP
    

    Use

    Donor template

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    Available since

    March 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCDNA3-human C-MYC-V394D

    Plasmid

    #74162

    Purpose

    Mammalian expression of human cMyc VD mutant

    Depositor

    Martine Roussel

    Article

    Vo et al Cancer Cell. 2016 Jan 11;29(1):5-16. doi

    Insert

    cMyc (MYC Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    V394D

    Promoter

    CMV

    Available since

    April 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1 LIC 6A CARD8-FLAG S297A

    Plasmid

    #169949

    Purpose

    Mammalian expression of CARD8-FLAG that is autoprocessing-deficient (S297A)

    Depositor

    Hao Wu

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S297A

    Promoter

    CMV

    Available since

    May 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1 6B CARD8 UPA-CARD-mCherry WT

    Plasmid

    #164011

    Purpose

    Mammalian expression vector encoding CARD8 UPA-CARD with a C-terminal mCherry tag for imaging

    Depositor

    Hao Wu

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDONR221 NLRP1 S1213A

    Plasmid

    #166824

    Purpose

    Gateway pDONR vector containing NLRP1 with the P1214R patient mutation (no stop codon)

    Depositor

    Daniel Bachovchin

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Gateway shuttling vector

    Tags

    Expression

    Mutation

    S1213A

    Promoter

    Available since

    March 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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