We narrowed to 14,071 results for: cas9

1. dCas9-CRY2Clust-BRD4ΔN

   Plasmid

   #200968

   Purpose

   induction of phase-separated condensate mediated by BRD4dN-IDR

   Depositor

   Jian Xu

   Article

   Kim et al Sci Adv. 2023 Mar 31;9(13):eadg1123. doi

   Insert

   BRD4dN
   

   Expression

   Bacterial and Mammalian

   Available Since

   Aug. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. L2_lacZgRNA-Cas9-CsA

   Plasmid

   #136138

   Purpose

   Plasmid to accept gRNA target with SapI, lacZ blue-white screening. Contains Cas9 and HygR.

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   p5-35Sx2:HygR p5-MpU6:lacZgRNA p5-MpEF1a:Cas9-NLS
   

   Use

   Synthetic Biology

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Available Since

   Nov. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. dCas9-NUP98a_IDR-EGFP

   Plasmid

   #232766

   Purpose

   Expresses dCas9-NUP98a_IDR-EGFP

   Depositor

   Isaac Hilton

   Article

   Li et al Nucleic Acids Res. 2025 Feb 8;53(4):gkaf

   Insert

   dCas9-NUP98a_IDR-EGFP (NUP98 Human)
   

   Use

   Lentiviral

   Promoter

   EF1a

   Available Since

   April 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEN017-SpCas9-hiNLS-t-M4

   Plasmid

   #234455

   Purpose

   Expresses hiNLS variant t-M4 inside 0C triNLS-SpCas9-NGG

   Depositor

   Ross Wilson

   Article

   Noel et al CRISPR J. 2025 Apr;8(2):105-119. doi: 10

   Insert

   SpCas9 hiNLS t-M4
   

   Use

   CRISPR

   Tags

   His6, Nucleoplasmin, SV40, and c-Myc

   Expression

   Bacterial

   Mutation

   Cys at positions 80 and 574 have been mutated to …

   Available Since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. dCas9-p300 HAT

   Plasmid

   #179544

   Purpose

   encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT domain (aa 1287-1664) driven by AF4 promoter

   Depositor

   Isaac Hilton

   Article

   Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

   Insert

   S. pyogenes dCas9 with c-terminal fusion of human p300 HAT domain (aa 1287-1664) (EP300 Human)
   

   Use

   Lentiviral

   Tags

   Flag Tag

   Expression

   Mammalian

   Mutation

   D10A; H840A in S.pyogenes Cas9

   Promoter

   EF-1alpha

   Available Since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV-P3-evoCjCas9-TadCDd_gRNA

   Plasmid

   #202563

   Purpose

   P3-evoCjCas9-TadCDd and hU6-BsmBI-gRNA in AAV2 backbone (ITRs)

   Depositor

   Gerald Schwank

   Article

   Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

   Insert

   evoCjCas9-TadCDd
   

   Use

   AAV

   Available Since

   Sept. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMH-Cas9-gate

   Plasmid

   #113742

   Purpose

   Gateway destination vector encoding Maize ubiquitin promoter driving Cas9 and 35S promoter driving hygromycin for selection in plants.

   Depositor

   Magdalena Bezanilla

   Article

   Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

   Insert

   Cas9
   

   Use

   CRISPR; Gateway destination vector

   Promoter

   Z. Mays ubiquitin promoter, 35S promoter

   Available Since

   Sept. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSET-dCas9-actII-4-NT-S1

   Plasmid

   #110185

   Purpose

   Repression of gene expression in Streptomyces by CRISPRi

   Depositor

   Yinhua Lu

   Article

   Zhao et al Biotechnol J. 2018 Jun 3. doi: 10.1002/b

   Inserts

   dCas9
   sgRNA
   

   Use

   CRISPR; I

   Expression

   Bacterial

   Mutation

   D10A, H840A

   Promoter

   J23119 and ermE*p

   Available Since

   July 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCas9-EcRT-Z3-Hyg

   Plasmid

   #232095

   Purpose

   Yeast CEN plasmid for estradiol-inducible expression of spCas9 and Ec86 reverse transcriptase.

   Depositor

   Jeffrey Lewis

   Article

   Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

   Insert

   Z3 promoter and Z3EV transcription factor
   

   Use

   CRISPR

   Expression

   Yeast

   Promoter

   Z3

   Available Since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. p221z-CAS9p-t35s

    Plasmid

    #118385

    Purpose

    Entry clone containing CAS9p and 35S terminator. Used to make CRISPR construct . For use in plants and compatible with the MultiSite Gateway system

    Depositor

    Ari Pekka Mähönen

    Article

    Wang et al Nat Plants. 2020 Jun 29. pii: 10.1038/s4

    Insert

    CAS9p
    

    Use

    CRISPR

    Available Since

    Sept. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti6.3-SaCas9-BlastR

    Plasmid

    #220966

    Purpose

    Expresses human codon-optimized SaCas9

    Depositor

    Hyongbum Kim

    Article

    Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

    Insert

    SaCas9
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. Rad51–Cas9(D10A)

    Plasmid

    #125567

    Purpose

    Expresses the Rad51–Cas9(D10A) fusion construct in mammalian cells under CMV promoter

    Depositor

    David Liu

    Article

    Rees et al Nat Commun. 2019 May 17;10(1):2212. doi:

    Insert

    Rad51–Cas9(D10A) (RAD51 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    May 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. PX458-3xHA-en31FnCas9ABEmax8.17d

    Plasmid

    #201956

    Purpose

    Mammalian expression plasmid of en31FnCas9ABEmax8.17d base editor with T2A-EGFP and cloning backbone for sgRNA

    Depositor

    Debojyoti Chakraborty

    Article

    Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

    Insert

    bpNLS-en31FnCas9ABEmax8.17d-bpNLS-3xHA-T2A-EGFP
    

    Use

    CRISPR

    Tags

    3xHA, NLS, and T2A-EGFP

    Expression

    Mammalian

    Promoter

    Cbh

    Available Since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pYPQ-dpcoCas9-Act3.0

    Plasmid

    #158408

    Purpose

    CRISPR-Act3.0 system containing dpcoCas9-VP64 fusion protein, T2A linked MS2-SunTag fusion protein, and ScFv-sfGFP-2xTAD activator.

    Depositor

    Yiping Qi

    Article

    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

    Insert

    dpcoCas9-VP64-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pdCas9-tdTomato-VPR

    Plasmid

    #232632

    Purpose

    VPR activation domain coupled to catalytically inactive dCas9 for localization; contains tdTomato for labeling and as a spacer

    Depositor

    Karsten Rippe

    Insert

    dCas9-tdTomato-VPR
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. eSpCas9(1.1)_No_FLAG_LMNA_G2

    Plasmid

    #178090

    Purpose

    Expresses the LMNA G2 sgRNA in combination with FLAGless eSpCas9(1.1). This vector can be used in combination with LMNA_mScarlet-I_Donor to tag LMNA with mScarlet-I. pX330-like plasmid.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    LMNA G2 sgRNA + FLAGless enhanced specificity Cas9 (1.1)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6 promoter

    Available Since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. eSpCas9(1.1)_No_FLAG

    Plasmid

    #79877

    Purpose

    FLAGless construct expressing high specificity eSpCas9(1.1). Px330-like plasmid.

    Depositor

    Yannick Doyon

    Article

    Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Untagged eSpCas9(1.1)

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    July 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. p-dCas9-VP64-Hygro

    Plasmid

    #104405

    Purpose

    transient expression of dCas9-VP64 fusion protein

    Depositor

    Stephan Beck

    Insert

    VP64
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV promoter

    Available Since

    April 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. G1397 DddAtox-N–dSpCas9

    Plasmid

    #157835

    Purpose

    expresses split DddAtox-Cas9 construct in mammalian cells

    Depositor

    David Liu

    Article

    Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

    Insert

    bpNLS–G1397 DddAtox-N–dSpCas9–UGI–UGI–bpNLS
    

    Use

    CRISPR

    Available Since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pX330-Flag-deSpCas9

    Plasmid

    #92114

    Purpose

    Expression plasmid for human codon-optimized dead/inactive increased fidelity eSpCas9 (1.1) (without U6-sgRNA coding sequence)

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Insert

    dead/inactive eSpCas9 with FLAG tag
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    D10A, H840A, K848A, K1003A, R1060A

    Promoter

    Cbh

    Available Since

    Oct. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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