We narrowed to 5,618 results for: cre encoding plasmid

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pJEP302-pAAV-CMV-dSaCas9-KRAB-pA

Plasmid

#113677

Purpose

A CMV driven de-catalyzed SaCas9 fused to KRAB domain for inhibition of transcription in targeted region.

Depositor

Jonathan Ploski

Article

Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

Insert

de-catalyzed SaCas9

Use

AAV and CRISPR

Tags

KRAB and NLS

Available Since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-FLEX-ArgiNLS-AausFP1

Plasmid

#220602

Purpose

Cre-dependent expression of a single-cell discriminating version of AausFP1 fluorescent protein.

Depositor

Sam Golden

Article

Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

Insert

AausFP1

Use

AAV and Cre/Lox

Tags

ArgiNLS

Expression

Mammalian

Promoter

CAG

Available Since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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pJEP305-pAAV-EFS-dSaCas9-KRAB-pA

Plasmid

#113681

Purpose

A EFS driven de-catalyzed SaCas9 fused to KRAB domain for inhibition of transcription in targeted region.

Depositor

Jonathan Ploski

Article

Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

Insert

de-catalyzed SaCas9

Use

AAV and CRISPR

Tags

KRAB and NLS

Available Since

Jan. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pJEP303-pAAV-CMV-dSaCas9-VP64-pA

Plasmid

#113678

Purpose

A CMV driven de-catalyzed SaCas9 fused to VP64 domain for increased transcription in targeted region

Depositor

Jonathan Ploski

Article

Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

Insert

de-catalyzed SaCas9

Use

AAV, CRISPR, and Synthetic Biology

Tags

NLS and VP64

Available Since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO {mCAR}off-{ChETA}on-WPRE

Plasmid

#111387

Purpose

AAV vector with hSynapsin promoter, Cre-OFF mCAR (for efficient CAV-2 infection) and Cre-ON ChETA (for optogenetic activation)

Depositor

Adam Kepecs

Article

Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi

Inserts

mCAR (Cxadr Mouse)
ChETA

Use

AAV

Tags

HA and Myc

Expression

Mammalian

Promoter

hSynapsin

Available Since

June 8, 2018

Availability

Academic Institutions and Nonprofits only

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pJEP312-pAAV-CMV-SaCas9-P2A-HAFLAGHA-KASH-pA

Plasmid

#113689

Purpose

SaCas9 driven by CMV. SaCas9 is followed by the self cleaving P2A sequence, several tags, and then the KASH transmembrane domain to enable FACS

Depositor

Jonathan Ploski

Article

Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

Insert

SaCas9 (NEWENTRY )

Use

AAV and CRISPR

Tags

Flag, HAx2, KASH, and NLS

Promoter

Cytomegalo Virus(CMV)

Available Since

Dec. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn1-DIO-Snca (human A53T)

Plasmid

#214201

Purpose

For Cre-dependent expression of alpha synuclein (human gene containing A53T mutation)

Depositor

Thomas Hnasko

Article

Steinkellner et al Brain. 2022 Apr 29;145(3):879-886. doi:

Insert

Snca (A53T) (SNCA Human)

Use

AAV and Cre/Lox

Expression

Mammalian

Mutation

A53T

Available Since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α-Flex/3'USS-ChRWR/Venus(ATG mut)

Plasmid

#197891

Purpose

Can be used to generate AAV virus that will express ChRWR/Venus in the presence of Cre and the leakage expression is significantly reduced without Cre

Depositor

Kazuto Kobayashi

Article

Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

Insert

ChRWR/Venus

Use

AAV

Mutation

N/A

Promoter

EF1a

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pJEP319-pAAV-U6SaCas9gRNA(emx1sg2)-EFS-GFP-KASH-pA

Plasmid

#113696

Purpose

U6 driven SaCas9 gRNA expression cassette with a gRNA targeting EMX1, followed by an EFS driven GFP-KASH in a separate reading frame.

Depositor

Jonathan Ploski

Article

Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

Inserts

SaCas9 gRNA Cassete
GFP

Use

AAV and CRISPR

Tags

KASH

Available Since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pJEP318-pAAV-U6SaCas9gRNA(emx1sg1)-EFS-GFP-KASH-pA

Plasmid

#113695

Purpose

U6 driven SaCas9 gRNA expression cassette with a gRNA targeting EMX1, followed by an EFS driven GFP-KASH in a separate reading frame.

Depositor

Jonathan Ploski

Article

Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

Inserts

SaCas9 gRNA Cassete
GFP

Use

AAV and CRISPR

Tags

KASH

Available Since

Jan. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-Fon/ConN2cG

Plasmid

#234709

Purpose

pAAV transfer plasmid with Ef1a promoter driving Cre- and Flp-dependent expression of G protein for rabies N2c delta G

Depositor

David Ng

Article

Mendelsohn et al bioRxiv [Preprint]. 2024 Sep 18:2024.08.

Insert

Fon Con N2cG

Use

AAV

Expression

Mammalian

Promoter

Ef1a

Available Since

March 27, 2025

Availability

Academic Institutions and Nonprofits only

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AAV.pCry1(408).DIO.Luciferase

Plasmid

#192623

Purpose

Cre-dependent expression of firefly luciferase, driven by a minimal mouse Cry1 promoter

Depositor

Michael Hastings

Article

Brancaccio et al Science. 2019 Jan 11;363(6423):187-192.

Insert

luciferase

Use

AAV

Expression

Mammalian

Available Since

Nov. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pB6-Id1-loxP-AU1-rox-FNF-loxP-drePBD*-rox

Plasmid

#168770

Purpose

B6J Id1 AU1 tag and LSL-TagRFP-T-drePBD* targeting vector, low copy number

Depositor

Mario Capecchi

Insert

Id1 (Id1 Mouse)

Use

Cre/Lox and Mouse Targeting; Dre / rox

Mutation

See Depositor Comments Below

Available Since

Aug. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-FLEX-Archon1-KGC-EGFP-ER2

Plasmid

#108422

Purpose

AAV production plasmid encoding for Archon1 fluorescent voltage reporter, Cre-dependent expression

Depositor

Edward Boyden

Article

Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

Has Service

AAV5

Insert

Archon1-KGC-EGFP-ER2

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

CAG

Available Since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

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hSyn-FAS-DO-ReaChR-mCitrine-WPRE

Plasmid

#64035

Purpose

Expresses ReaChR under hSyn promoter. FAS-DO turns off expression in Cre-positive cells. FAS is compatible with other traditional Flex/DIO constructs

Depositor

Karel Svoboda

Insert

hSyn-FAS-DO-ReaChR-mCitrine-WPRE

Use

AAV

Tags

ReaChR tagged with mCitrine

Expression

Mammalian

Promoter

hSyn

Available Since

May 27, 2015

Availability

Academic Institutions and Nonprofits only
pCMV-FLEx-TetOn3G-3xHA-FLEx

Plasmid

#133729

Purpose

Plasmid contains the floxed TetOn3G-transactivator sequence for expression of TetOn3G in the presence of Cre recombinase.

Depositor

Erin Schuman

Article

Heumuller et al Nat Methods. 2019 Jul 15. pii: 10.1038/s

Insert

TetOn3G-3xHA (floxed & inverted)

Use

AAV

Tags

3xHA-tag

Expression

Mammalian

Promoter

pCMV

Available Since

Dec. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-DIO-hM3Dq-mCherry

Plasmid

#166599

Purpose

Encodes Cre-dependent hM3Dq-mCherry under control of the TRE

Depositor

Martin Myers

Article

Sabatini et al Elife. 2021 Mar 11;10. pii: 66835. doi:

Insert

hM3Dq-mCherry

Use

AAV

Promoter

tetracycline response element

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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PB-eDIO-EYFP

Plasmid

#104388

Purpose

PiggyBac plasmid encoding EYFP in an inverse orientation flanked by loxP and lox2722 sites under the control of the EF1a promotor.

Depositor

Volker Busskamp

Article

Klapper et al Sci Rep. 2017 Oct 31;7(1):14450. doi: 10

Insert

Enhanced Yellow Fluorescent Protein

Use

Cre/Lox; Piggybac

Expression

Mammalian

Available Since

Jan. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pATF4.1.0-gDNA

Plasmid

#113772

Purpose

CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ATF4

Depositor

Kevin White

Insert

ATF4 (ATF4 Human)

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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AAV CAG-FLEx-Rpl22-3xHA

Plasmid

#245798

Purpose

Cre-dependent expression of Rpl22-HA

Depositor

Baljit Khakh

Insert

Ribosomal protein L22 (Rpl22 Mouse)

Use

AAV

Tags

3xHA

Promoter

CAG

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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