243,317 results

1. Z-lock cofilin

   Plasmid

   #141137

   Purpose

   controlling the actin-severing ability of cofilin with light

   Depositor

   Klaus Hahn

   Article

   Stone et al Nat Chem Biol. 2019 Dec;15(12):1183-1190

   Insert

   Zdk2, Cofilin, LOV2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   mCherry, Zdk2(132F), Cofilin S3A, LOV WT

   Promoter

   Available Since

   Dec. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-nEF-Con/Fon-ChRmine-oScarlet (AAV8)

   Viral Prep

   #137159-AAV8

   Purpose

   Ready-to-use AAV8 particles produced from pAAV-nEF-Con/Fon-ChRmine-oScarlet (#137159). In addition to the viral particles, you will also receive purified pAAV-nEF-Con/Fon-ChRmine-oScarlet plasmid DNA. nEF-driven, Cre and Flp-dependent expression of ChRmine-oScarlet. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Karl Deisseroth, INTRSECT 2.0 Project

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Promoter

   nEF

   Tags

   oScarlet (Cre and Flp-dependent)

   Available Since

   Sept. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

3. pAAV.TBG.PI.eGFP.WPRE.bGH

   Plasmid

   #105535

   Purpose

   AAV expression of EGFP from TBG promoter

   Depositor

   James M. Wilson

   Has Service

   AAV8

   Insert

   EGFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TBG

   Available Since

   Feb. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV.TBG.PI.eGFP.WPRE.bGH

   Plasmid

   #105535

   Purpose

   AAV expression of EGFP from TBG promoter

   Depositor

   James M. Wilson

   Has Service

   AAV8

   Insert

   EGFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TBG

   Available Since

   Feb. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. IFNAR1_pCSdest

   Plasmid

   #53881

   Depositor

   Roger Reeves

   Article

   Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

   Insert

   IFNAR1 (IFNAR1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SP6 and CMV

   Available Since

   March 27, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. IFNAR1_pCSdest

   Plasmid

   #53881

   Depositor

   Roger Reeves

   Article

   Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

   Insert

   IFNAR1 (IFNAR1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SP6 and CMV

   Available Since

   March 27, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pZR071_SFFV-dCas9-mCherry-KRAB

   Plasmid

   #180264

   Purpose

   Lentiviral expression vector for dCas9-KRAB in mammalian cells

   Depositor

   Alexander Marson

   Article

   Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

   Insert

   dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   KRAB and mCherry

   Expression

   Mammalian

   Mutation

   Nuclease dead Cas9

   Promoter

   SFFV

   Available Since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGW-GOI-TurboID

   Plasmid

   #209378

   Purpose

   Gateway compatible plasmid for TurboID-based proximity labelling experiment in planta

   Depositor

   Rosa Lozano-Durán

   Article

   Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   TurboID gene sequence C249A

   Promoter

   Available Since

   Nov. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. zCas9i cloning kit: MoClo-compatible CRISPR/Cas9 cloning kit with intronized Cas9

   Plasmid Kit

   #1000000171

   Purpose

   Vectors and DNA modules for assembly of CRISPR/Cas9 constructs for use in plants. The constructs are made with zCas9i, a high efficiency intron-optimized SpCas9-coding gene.

   Depositor

   Johannes Stuttmann, Sylvestre Marillonnet

   Available Since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

10. pRDA_221

    Plasmid

    #169142

    Purpose

    Constitutive expression of EGFP and sgRNAs targeted to EGFP for measurement of activity of AsCas12a\EnAsCas12a.

    Depositor

    John Doench, David Root

    Insert

    EGFP , EGFP sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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