165,888 results

1. Gi1-CASE

   Plasmid

   #168120

   Purpose

   Encodes a BRET-based activity sensor for the heterotrimeric G protein Gi1. Composed of the subunits G alpha i1 (GNAI1) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 2 (GNG2).

   Depositor

   Gunnar Schulte

   Article

   Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.

   Insert

   GNAI1, GNB1, GNG2 (GNB1, GNG2, GNAI1 Human)
   

   Use

   Luciferase

   Tags

   cpVenus on GNG2

   Expression

   Mammalian

   Mutation

   NLuc is inserted at A121/E122 within GNAI1

   Promoter

   Available Since

   July 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. TetON-shYAP

   Plasmid

   #115667

   Purpose

   Inducible YAP knockdown

   Depositor

   Joan Massague

   Article

   Er et al Nat Cell Biol. 2018 Aug;20(8):966-978. d

   Insert

   YAP shRNA (YAP1 Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHR_PGK_SNIPR_Hinge Notch SNIPR (Notch2 JMD)

   Plasmid

   #188375

   Purpose

   Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD, a Notch2-JMD, and a Gal4VP64 transcriptional factor

   Depositor

   Kole Roybal

   Article

   Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

   Insert

   PGK_antiCD19_CD8alpha-variant2-ECD_Notch1-TMD_Notch2-JMD_Gal4VP64
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCRIS-PITChv2-Puro-dTAG (BRD4)

   Plasmid

   #91793

   Purpose

   PITCh dTAG donor vector for Puro-P2A-2xHA-FKBP_F36V knock-in into the N-terminus of the human BRD4 locus.

   Depositor

   Behnam Nabet, James Bradner

   Article

   Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

   Insert

   Puro-P2A-2xHA-FKBP_F36V
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Phenylalanine 36 to valine in FKBP12

   Promoter

   Promotorless

   Available Since

   March 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV5-Smad7-HA

   Plasmid

   #11733

   Depositor

   Jeff Wrana

   Article

   Hayashi et al Cell. 1997 Jun 27. 89(7):1165-73.

   Insert

   SMAD7 (SMAD7 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 12, 2006

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. EF1a-dCas9-KRAB-GFP backbone

   Plasmid

   #194281

   Purpose

   Vector for CRISPRi-GFP expression ready for gateway cloning of sgRNA

   Depositor

   Elizabeth Kirby

   Article

   Rieskamp et al iScience. 2023 Jun 8;26(7):107068. doi:

   Insert

   dCas9-KRAB-GFP
   

   Use

   CRISPR and Lentiviral

   Tags

   GFP

   Expression

   Mutation

   Promoter

   EF1a

   Available Since

   June 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTn Donor

   Plasmid

   #236211

   Purpose

   Arabinose-inducible Tn5 InducTn-seq plasmid

   Depositor

   Matthew Waldor

   Article

   Basta et al Nat Microbiol. 2025 Mar 27. doi: 10.1038

   Insert

   None
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRK5-HA-Parkin

   Plasmid

   #17613

   Depositor

   Ted Dawson

   Article

   Zhang et al Proc Natl Acad Sci U S A 2000 Nov 21;97(

   Insert

   Parkin (PRKN Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 3, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSP2-48-merTetO-EFS-BLaR

   Plasmid

   #118712

   Purpose

   Contains an optimized 48-mer TetO repeat for imaging of desired loci

   Depositor

   Huimin Zhao

   Article

   Tasan et al Nucleic Acids Res. 2018 Jun 15. pii: 503

   Inserts

   Blasticidin resistance gene
   EFS-NS promoter
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EFS-NS

   Available Since

   Feb. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG_smFP HA

    Plasmid

    #59759

    Purpose

    encodes multiple copies of an epitope tag HA

    Depositor

    Loren Looger

    Article

    Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

    Insert

    smFP _HA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Oct. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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