163,852 results

1. R619-M89-303: CMV51p> HCoV-HKU1 S-2P-T4f-3C-His8-Strep2x2

   Plasmid

   #166014

   Purpose

   mammalian expression of HCoV-HKU1 soluble spike trimer protein

   Depositor

   Dominic Esposito

   Article

   Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

   Insert

   HCoV-HKU1 S (spike)
   

   Use

   Tags

   3C-His8-Strep2x2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV51

   Available since

   March 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. GalT-RpHLuorin2

   Plasmid

   #171719

   Purpose

   Trans-Golgi expression of ratiometric pHLuorin2

   Depositor

   Geert van den Bogaart

   Article

   Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

   Insert

   Ratiometric pHLuorin2 fused to B4GALT1 (B4GALT1 Human)
   

   Use

   Tags

   ratiometric pHluorin2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. BIC-Gag-CAS9

   Plasmid

   #119942

   Purpose

   encodes a GAG (F-MLV)-CAS9(sp) fusion. Allows the production of GAG-CAS9 Virus like particles from producer cells in association with over expressed gRNA(s) and appropriate envelopes.

   Depositor

   Emiliano Ricci, Théophile Ohlmann, Philippe Mangeot

   Article

   Mangeot et al Nat Commun. 2019 Jan 3;10(1):45. doi: 10

   Insert

   GAG FMLV fused to spCAS9
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   hCMV

   Available since

   April 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. SUPERBLADE5

   Plasmid

   #134913

   Purpose

   Empty backbone for cloning sgRNA sequence to be used in nanoblades system (Optimized for increased genome editing efficiency via Chen B et al., 2013)

   Depositor

   Emiliano Ricci, Théophile Ohlmann, Philippe Mangeot

   Article

   Mangeot et al Nat Commun. 2019 Jan 3;10(1):45. doi: 10

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Dec. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. qTAG-N-Puro-dTAG

   Plasmid

   #207694

   Purpose

   Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-dTAG cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. p1303 DV-SFTPC-tdTomato

   Plasmid

   #126696

   Purpose

   donor vector for targeting a tdTomato reporter to the human SFTPC locus at the endogenous ATG start site

   Depositor

   Darrell Kotton

   Article

   Jacob et al Cell Stem Cell. 2017 Oct 5;21(4):472-488

   Insert

   tdTomato
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-CMV-SauriCas9

   Plasmid

   #135964

   Purpose

   Expresses SauriCas9, and cloning backbone for sgRNA

   Depositor

   Yongming Wang

   Article

   Hu et al PLoS Biol. 2020 Mar 30;18(3):e3000686. d

   Type

   Empty backbone

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. P4-TadCBEd

   Plasmid

   #225143

   Purpose

   Packaging plasmid for TadCBEd in v3b PE-eVLP architecture

   Depositor

   David Liu

   Article

   An et al Nat Biotechnol. 2024 Jan 8. doi: 10.1038

   Insert

   P4-TadCBEd
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-CBE4max-SpRYc-P2A-EGFP

   Plasmid

   #208336

   Purpose

   CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpRYc

   Depositor

   Pranam Chatterjee

   Article

   Zhao et al Nat Commun. 2023 Oct 4;14(1):6175. doi:

   Insert

   human codon optimized CBE4max Cas9 variant named SpRYc with P2A-EGFP
   

   Use

   CRISPR

   Tags

   P2A-EGFP

   Expression

   Mammalian

   Mutation

   Sc++ (1-1119), SpRY (1111-1368), D10A

   Promoter

   CAG

   Available since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMXs GFP-LC3-RFP

    Plasmid

    #117413

    Purpose

    Expresses GFP-LC3-RFP in mammalian cells to measure autophagic flux without any drug resistance.

    Depositor

    Noboru Mizushima

    Article

    Morita et al J Cell Biol. 2018 Aug 9. pii: jcb.201804

    Insert

    microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
    

    Use

    Retroviral

    Tags

    EGFP and mRFP1

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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