161,601 results

1. pInducer20 CARD8-HA PAM

   Plasmid

   #169982

   Purpose

   Tet/Dox-inducible mammalian expression of CARD8 with sgRNA binding site mutations

   Depositor

   Daniel Bachovchin

   Article

   Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

   Insert

   CARD8 (CARD8 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   PAM

   Promoter

   Available since

   May 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHAGE-POU4F2

   Plasmid

   #116776

   Purpose

   Lentiviral expression of POU4F2

   Depositor

   Gordon Mills, Kenneth Scott

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   POU4F2 (POU4F2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. mEos3.2FPP

   Plasmid

   #203758

   Purpose

   Encodes the membrane anchor of HRas, including the last 10 residues of the C terminus with 1 farnesylation and 2 palmitoylations, with mEos3.2 fused to the N terminus. Used as a fluorescent plasma membrane probe.

   Depositor

   Sarah Veatch

   Article

   Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d

   Insert

   HRas c-term (HRAS Human)
   

   Use

   Tags

   mEos3.2

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. ePPE-V223A

   Plasmid

   #205243

   Purpose

   For plant prime editing in wheat plants or monocotyledons protoplasts

   Depositor

   Zong Yuan

   Article

   Ni et al Genome Biol. 2023 Jun 29;24(1):156. doi:

   Insert

   SV40_NLS-nCas9-NC-MLV-SV40_NLS
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Detailed in manuscript

   Promoter

   Available since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. Double UP mNeon to mScarlet Rac1-V12

   Plasmid

   #125136

   Purpose

   Natively produces mNeonGreen, in the presence of Cre will be recombined to produce mScarlet and an untagged Rac1-V12 (Constitutively Active).

   Depositor

   Erik Dent

   Article

   Taylor et al Front Mol Neurosci. 2020 May 20;13:82. d

   Insert

   mNeon/mScarletP2A Rac1-V12
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Removed Not1 site in mScarlet. 12th AA in Rac1 ch…

   Promoter

   CAG

   Available since

   May 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pU6-SasgRNA scaffold-CMV-nSaCas9

   Plasmid

   #171695

   Purpose

   Expresses nSaCas9 in mammalian cells

   Depositor

   Jia Chen

   Article

   Wang et al Nat Cell Biol. 2021 May 10. pii: 10.1038

   Insert

   sgRNA scaffold-nSaCas9-NLS
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   U6 and CMV

   Available since

   Aug. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. RAB11A-mCherry HDRT Source (pTR 144)

   Plasmid

   #112013

   Purpose

   DNA sequence source for amplifying an HDR template to tag endogenous human RAB11A gene with mCherry

   Depositor

   Alexander Marson

   Article

   Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

   Insert

   RAB11A-mCherry HDRT (RAB11A Human)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHH0103_GNAI2_G-alpha

   Plasmid

   #109791

   Purpose

   Protein expression and purification of GNAI2_G-alpha

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

   Insert

   GNAI2_G-alpha (GNAI2 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEN_TT 3xFLAG-CSE1L

   Plasmid

   #192295

   Purpose

   Gateway entry vector for an inducible 3xFLAG-CSE1L

   Depositor

   Kevin Janes

   Article

   Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

   Insert

   CSE1L (CSE1L Human)
   

   Use

   Gateway entry vector

   Tags

   3xFLAG

   Expression

   Mutation

   Promoter

   Available since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. MRGPRD-Tango

    Plasmid

    #66435

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    MRGPRD (MRGPRD Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only