We narrowed to 20,614 results for: ACE
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Plasmid#47645PurposepAC-EsaR with a c659t mutation in esaRDepositorInsertesaR-V220A
UseSynthetic BiologyExpressionBacterialMutationChanged Valine 220 to AlaninePromoterPlacAvailable SinceSept. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMZ2007
Plasmid#204399PurposeA 3OC6-HSL sensor for B. subtilis on a shuttle vector for B. subtilis and E. coli.DepositorInsertsquorum sensing regulator LuxR
GFP
UseSynthetic BiologyPromoterPftsH and Synthetic promoter PLux0002Available SinceDec. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUPD2+pG10-90
Plasmid#170879PurposePhytobrick - pG10-90DepositorInsertpG10-90
ExpressionPlantAvailable SinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQE-WT-CTGA-R13-AKSIRV
Plasmid#63627PurposeCTGA-R13-AKSIRV RNAP mutantDepositorInsertCTGA-R13-AKSIRV RNAP
Tagsn/aMutationV725A, Q744K, L747I, N748S, L749I, R756T, Q758K, …PromoterT5/LacAvailable SinceJune 3, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUC19-Split-Broccoli-Toehold-Switch
Plasmid#87316PurposeExpresses the Split-Broccoli system and a Trigger/Toehold switch for mCherry.DepositorInsertSplit-Broccoli system and RNA Trigger (TS2_AT01) Toehold (TS2_KS01) switch for mCherry
UseSynthetic BiologyExpressionBacterialPromoterP70aAvailable SinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
BASIC_7_J23101-RBS34-mCherry-B0015
Plasmid#68141Purposestorage vector for BASIC part: RFP expression cassetteDepositorInsertJ23101-RBS34-mcherry-B0015
ExpressionBacterialAvailable SinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pQE-WT-N4-R5-RNRV
Plasmid#63630PurposeN4-R5-RNRV RNAP mutantDepositorInsertN4-R5-RNRV RNAP
MutationL747I, N748D, L749C, M750V, F751I, Q754T, F755R, …PromoterT5/LacAvailable SinceApril 15, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBP-TL5
Plasmid#72989PurposeRBS_M2DepositorInsertM2 RBS
UseSynthetic BiologyMutationCAT gene - C435G (nucleotide) - silent mutagenesi…Available SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBP-TL11
Plasmid#72995PurposeRBS_L4DepositorInsertL4 RBS
UseSynthetic BiologyMutationCAT gene - C435G (nucleotide) - silent mutagenesi…Available SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
CDS_aadA_chlo
Plasmid#163935PurposeL0 part - CDSDepositorInsertaadA gene for spectinomycin resistance
ExpressionBacterialAvailable SinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMM705
Plasmid#68892PurposeIPTG-inducible CRISPRi vector targeting BT1754, pNBU2 backbone, AmpRDepositorInsertsdCas9
LacI
sgRNA
Available SinceOct. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFTK046
Plasmid#171318PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertDsRed.T1 fluorescent reporter
UseSynthetic BiologyExpressionBacterial and YeastMutationn/aAvailable SinceDec. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
Golgi-DKAR
Plasmid#49790PurposeFRET-based, Golgi-targeted kinase activity reporter for PKDDepositorInsertD kinase activity reporter
TagsCFP, YFP, and eNOS(1-33)ExpressionMammalianPromoterCMVAvailable SinceJan. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSecEffector
Plasmid#172891PurposeContains TEVp display cassette, atc inducible (in any E. coli strain) BxbI and arabinose inducible TP901DepositorInsertsBxb1
TP901
TEV Display cassette
ProD promoter between anti aligned Bxb1 sites
ExpressionBacterialPromoterProD, nTETO, and pBAD /AraCAvailable SinceJuly 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQLinkHD_EBFP2
Plasmid#118853Purposefluorescent protein expression with an N-terminal His-tagDepositorInsertEBFP2
TagsHis-tagExpressionBacterialMutationEBFP1.2-I128V/V150I/ D155V/V224RAvailable SinceDec. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZA2[PTAN:GFP]
Plasmid#60779PurposeContains PTAN driving expression of GFPDepositorInsertyemGFP
Available SinceApril 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
NAT-TET-OFF -LEU- 4xFLAG
Plasmid#182527PurposeDoxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xFLAG N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.DepositorInsert7 Copies of TetO Box
Tags4XFLAGExpressionYeastAvailable SinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET21-tVYN-Nt∆
Plasmid#154431PurposePlasmid used to express the tVYN-Nt∆ luminescent biosensor for use in live cell cyclic di-GMP detectionDepositorInsertVenusΔC12-NtYcgRΔ-NLucΔ4
ExpressionBacterialPromoterT7Available SinceAug. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFTK056
Plasmid#171328PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertSpCas9-NLS
UseSynthetic BiologyExpressionBacterial and YeastMutationn/aAvailable SinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBbB13k-GFP
Plasmid#126122PurposeConstitutive expression vector for Escherichia coli. BglBrick compatible.DepositorInsertGFPuv
UseSynthetic BiologyExpressionBacterialPromoterJ23102Available SinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBLlSh2
Plasmid#170981PurposeExpression of ShCAST under control of lacI Plac promoter/repressor pair.DepositorInsertslacI
ShTnsB, ShTnsC, ShTniQ, ShCas12k
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterpLlac01Available SinceJuly 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHelper_O
Plasmid#173783PurposeThe 186 helper plasmid necessary for genomic integration of the pIT5_KO plasmid.DepositorInsert186 Integrase
UseSynthetic BiologyExpressionBacterialMutationE59G - please see depositor commentsAvailable SinceDec. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHelper_W
Plasmid#173784PurposeThe Wphi helper plasmid necessary for genomic integration of the pIT5_KW plasmid.DepositorInsertWphi Integrase
UseSynthetic BiologyExpressionBacterialAvailable SinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHelper_D
Plasmid#173781PurposeThe D145 helper plasmid necessary for genomic integration of the pIT5_KD plasmid.DepositorInsertD145 Integrase
UseSynthetic BiologyExpressionBacterialAvailable SinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFTK088
Plasmid#171360PurposeAMA1 sequence as Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertAMA1 sequence (short), entry vector providing fungal replication
UseSynthetic BiologyExpressionBacterial and YeastMutationn/aAvailable SinceJan. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFTK019
Plasmid#171291PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertPTET-10x-repeat promoter+CPgpdA (fused)
UseSynthetic BiologyExpressionBacterial and YeastMutationn/aAvailable SinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFTK017
Plasmid#171289PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertPTET-5x-repeat promoter+CPgpdA (fused)
UseSynthetic BiologyExpressionBacterial and YeastMutationn/aAvailable SinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFTK038
Plasmid#171310PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInserthph, hygR, hygromycin selection marker
UseSynthetic BiologyExpressionBacterial and YeastMutationn/aAvailable SinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFTK037
Plasmid#171309PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertbleoR phleomycin, selection marker
UseSynthetic BiologyExpressionBacterial and YeastMutationn/aAvailable SinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pZA2[PLac:mCherry,PTAN:GFP]
Plasmid#60780PurposeContains PLac driving expression of mCherry and PTAN driving expression of GFP.DepositorInsertsmCherry
yemGFP
Available SinceApril 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
TDGS50
Plasmid#183189PurposeTEV protease expressed under J23106 promoter; DivIVAsfGFP-TEV protease recognition site-M0050 expressed under J23116 promoterDepositorInsertsDivIVA-GFP(tevR M0050)
TEV-S219V
ExpressionBacterialAvailable SinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFTK084
Plasmid#171356PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertTtRNA[Met] A.niger Pol-III terminator
UseSynthetic BiologyExpressionBacterial and YeastMutationn/aAvailable SinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFTK039
Plasmid#171311PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertpyrG AO090011000868 Orotidine 5'-phosphate decarboxylase, selection marker
UseSynthetic BiologyExpressionBacterial and YeastMutationn/aAvailable SinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUCSh3-mRFP
Plasmid#170983PurposeEncodes sgRNA scaffold with mRFP in place of target and Tetracycline resistance transposon. New targets can be cloned replacing mRFP using around the horn cloning.DepositorInsertmRFP
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterJ23119Available SinceJuly 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFTK080
Plasmid#171352PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertTcyc1 YJR048W terminator
UseSynthetic BiologyExpressionBacterial and YeastMutationn/aAvailable SinceJan. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFTK079
Plasmid#171351PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertToat1 Pc18g03600 terminator
UseSynthetic BiologyExpressionBacterial and YeastMutationn/aAvailable SinceJan. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFTK052
Plasmid#171324PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertFirefly luciferase reporter
UseSynthetic BiologyExpressionBacterial and YeastMutationn/aAvailable SinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFTK049
Plasmid#171321PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertmCherry fluorescent reporter
UseSynthetic BiologyExpressionBacterial and YeastMutationn/aAvailable SinceDec. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR APLP2 shRNA 3
Plasmid#30161DepositorInsertAPLP2 shRNA 3
ExpressionMammalianAvailable SinceJuly 28, 2011AvailabilityAcademic Institutions and Nonprofits only -
pFTK051
Plasmid#171323PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInserteBFP fluorescent reporter
UseSynthetic BiologyExpressionBacterial and YeastMutationn/aAvailable SinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only