We narrowed to 4,411 results for: Ado;

1. VPS13B^GFP

   Plasmid

   #224588

   Purpose

   codon optimized GFP tagged VPS13B for expression in mammalian cells

   Depositor

   Pietro De Camilli

   Article

   Ugur et al bioRxiv [Preprint]. 2023 Dec 18:2023.12.

   Insert

   VPS13B^GFP (VPS13B Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRN3P-NLS-EGFP-deadBtgh-3NLS

   Plasmid

   #194470

   Purpose

   Encodes for catalytically inactive bacterial O-GlcNAcase BtGH84 fused to enhanced GFP and NLSs for effective nuclear localization, in backbone for T3 In Vitro Transcription.

   Depositor

   Mathieu Boulard

   Article

   Formichetti et al Dev Cell 10 Jan 2024

   Insert

   BtGH84
   

   Use

   For in vitro transcription from t3 promoter after…

   Tags

   SV40 Nuclear Localization Signal, SV40 Nuclear Lo…

   Expression

   Mutation

   Aspartic Acid 242 (in Dennis et al.) changed to A…

   Promoter

   Available since

   Jan. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-SypHer2-DAAO-NES

   Plasmid

   #119184

   Purpose

   Fusion of the pH-sensitive fluorescent biosensor SypHer2 with D-amino acid oxidase; excluded from nucleus. CMV promoter. Can serve as a pH control for HyPer.

   Depositor

   Thomas Michel

   Article

   Steinhorn et al Nat Commun. 2018 Oct 2;9(1):4044. doi: 1

   Insert

   SypHer2-DAAO-NES
   

   Use

   AAV

   Tags

   NES

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-GFAP-mKate2.5f

   Plasmid

   #99129

   Purpose

   Also known as pAAV-GFAP-mKate2.5-f. An AAV genome that express the farnesylated fluorescent protein mKate2.5 from the GFAP promoter

   Depositor

   Viviana Gradinaru

   Article

   Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

   Insert

   mKate2.5f
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   GFAP

   Available since

   April 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCS2+ Zf GFP-vclb

   Plasmid

   #185411

   Purpose

   GFP tagged form of zebrafish vinculin b. For in vitro transcription (SP6) or expression through CMV

   Depositor

   Nicolas David

   Article

   Boutillon et al Dev Cell. 2022 May 17. pii: S1534-5807(2

   Insert

   vinculin b (vclb Zebrafish)
   

   Use

   In vitro synthesis of mrna

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCS2+ Zf GFP-vcla

   Plasmid

   #185410

   Purpose

   GFP tagged form of zebrafish vinculin a. For in vitro transcription (SP6) or expression through CMV

   Depositor

   Nicolas David

   Article

   Boutillon et al Dev Cell. 2022 May 17. pii: S1534-5807(2

   Insert

   vinculin a (vcla Zebrafish)
   

   Use

   In vitro synthesis of mrna

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEf1a OsTIR ires NEO pA T2BH

   Plasmid

   #127910

   Purpose

   Transposon vector containing an OsTIR IRES Neo cassette driven by the Ef1a promoter

   Depositor

   Mark Groudine

   Article

   Strom et al Elife. 2021 Jun 9;10. pii: 63972. doi: 1

   Insert

   OsTIR
   

   Use

   Transposon

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAC93-pmax-dCas9VP160

   Plasmid

   #48225

   Purpose

   dCas9VP160 on pmax expression vector

   Depositor

   Rudolf Jaenisch

   Article

   Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

   Insert

   dCas9(D10A;H840A) fusion with VP160 activation domain
   

   Use

   CRISPR

   Tags

   HA Tag and VP160

   Expression

   Mammalian

   Mutation

   D10A;H840A (catalytically inactive)

   Promoter

   CAGGS

   Available since

   Sept. 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. U6-NT sgRNA; EF1a-dCas9-KRAB-GFP

   Plasmid

   #194284

   Purpose

   EF1a-dCas9-KRAB-GFP with nontarget sgRNA

   Depositor

   Elizabeth Kirby

   Article

   Rieskamp et al iScience. 2023 Jun 8;26(7):107068. doi:

   Insert

   dCas9-KRAB-GFP
   

   Use

   CRISPR and Lentiviral

   Tags

   GFP

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   June 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. U6-CjCas9-MS2gRNA-Backbone

    Plasmid

    #210711

    Purpose

    This plasmid express MS2 loop containing Cj Cas9 gRNA

    Depositor

    Isaac Hilton

    Article

    Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

    Insert

    MS2 loop containing Cj Cas9 gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. beta Synuclein-VC

    Plasmid

    #89484

    Purpose

    expresses human beta Synuclein fused to the C-terminal part of Venus

    Depositor

    Tiago Outeiro

    Article

    Tenreiro et al Hum Mol Genet. 2016 Jan 15;25(2):275-90.

    Insert

    SNCB (SNCB Human)
    

    Use

    Tags

    c-terminal part of Venus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. 138-dCas9-Dnmt3a

    Plasmid

    #84570

    Purpose

    PiggyBac transposon system construct with dCas9-Dnmt3a

    Depositor

    Rudolf Jaenisch

    Article

    Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

    Insert

    dCas9-Dnmt3a (DNMT3A Synthetic, Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEYFP-N1-A2BR

    Plasmid

    #37202

    Depositor

    Robert Tarran

    Article

    Watson et al FASEB J. 2011 Sep;25(9):2996-3003. Epub

    Insert

    A2BR (ADORA2B Human)
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEF-ManII-GFP

    Plasmid

    #160905

    Purpose

    Expresses the Golgi marker ManII-GFP in mammalian cells

    Depositor

    Benjamin Glick

    Article

    Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

    Insert

    MAN2A1 (MAN2A1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    Nov. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. mCh-LgBiT-2A-EGFP-Vin-PILATeS

    Plasmid

    #216761

    Purpose

    PiggyBac vector for stable co-expression of mCherry-LgBiT and EGFP-Vinculin-PILATeS (700 pM) in mammalian cells. Requires transposase

    Depositor

    Alex Dunn

    Article

    Zhong et al ACS Sens. 2024 Jul 26;9(7):3489-3495. do

    Insert

    mCherry-LgBiT-P2A-T2A-EGFP-Vin-PILATeS
    

    Use

    Tags

    EGFP and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. EF1a_MYOD1_P2A_Hygro_Barcode

    Plasmid

    #120464

    Purpose

    Barcoded lentiviral vector to express MYOD1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    MYOD1 (MYOD1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. EF1a_SRY_P2A_Hygro_Barcode

    Plasmid

    #120485

    Purpose

    Barcoded lentiviral vector to express SRY in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    SRY (SRY Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. ePB-Bsd-CAG-LoxP-dsRed_PA-LoxP-SV40-NLS-NG-T2A-hSHH

    Plasmid

    #179586

    Purpose

    ePiggyBac vector that has a blasticidin selectable cassette and a LoxP exchangeable dual colors cassette with SHH expression (Red module-dsRed/Green module-NeonGreen T2A SHH)

    Depositor

    Ali Hemmati Brivanlou

    Article

    De Santis et al Nat Commun. 2021 Nov 19;12(1):6768. doi:

    Insert

    NLS-NG-T2A-hSHH
    

    Use

    Piggybac

    Tags

    NeonGreen

    Expression

    Mutation

    Promoter

    TRE

    Available since

    Feb. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA5FRT/TO-UBE2QL1-Strep/HA

    Plasmid

    #124666

    Purpose

    Expresses UBE2QL1 with C-terminal Strep-HA tag in mammalian cells

    Depositor

    Hemmo Meyer

    Article

    Koerver et al EMBO Rep. 2019 Aug 21:e48014. doi: 10.15

    Insert

    UBE2QL1 (UBE2QL1 Human)
    

    Use

    Tags

    2xStrep/HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAC2-dual-dCas9VP48-sgExpression

    Plasmid

    #48236

    Purpose

    Dual expression construct expressing both dCas9VP48 and sgRNA from separate promoters

    Depositor

    Rudolf Jaenisch

    Article

    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

    Insert

    dCas9VP48
    

    Use

    CRISPR

    Tags

    HA-tag and VP48

    Expression

    Mammalian

    Mutation

    D10A H840A (catalytically inactive)

    Promoter

    Available since

    Sept. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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