We narrowed to 16,171 results for: NUC

1. pUC19-B5XLC1

   Plasmid

   #103088

   Purpose

   Cas9 coding gene template with optimized sequence for human codon usage

   Depositor

   Duhee Bang

   Article

   Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

   Insert

   B5XLC1(Cas9 coding gene from Streptococcus pyogenes serotype M49 (strain NZ131))
   

   Use

   CRISPR; Cloning vector

   Tags

   Expression

   Mutation

   human codon-optimized

   Promoter

   Available since

   April 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUC19-C4ZA16

   Plasmid

   #103090

   Purpose

   Cas9 coding gene template with optimized sequence for human codon usage

   Depositor

   Duhee Bang

   Article

   Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

   Insert

   C4ZA16(Cas9 coding gene from Eubacterium rectale (strain ATCC 33656 / VPI 0990))
   

   Use

   CRISPR; Cloning vector

   Tags

   Expression

   Mutation

   human codon-optimized

   Promoter

   Available since

   April 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-HA-LMW-FGF2

   Plasmid

   #157663

   Purpose

   Expresses FGF2 in mammalian cell

   Depositor

   Byung Il Lee

   Article

   Bong et al Nucleic Acids Res. 2020 Jun 19;48(11):63

   Insert

   Fibroblast growth factor 2 (FGF2 Human)
   

   Use

   Tags

   HA tag

   Expression

   Mammalian

   Mutation

   deleted amino acid 1-134

   Promoter

   Available since

   Aug. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA6 asyn WT

   Plasmid

   #107425

   Purpose

   Mammalian expression of human alpha synuclein

   Depositor

   Hilal Lashuel

   Article

   Mbefo et al J Biol Chem. 2015 Apr 10;290(15):9412-27

   Insert

   alpha-synuclein (SNCA Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA6 asyn E46K

   Plasmid

   #105730

   Purpose

   Mammalian expression of mutant human alpha synuclein

   Depositor

   Hilal Lashuel

   Article

   Mbefo et al J Biol Chem. 2015 Apr 10;290(15):9412-27

   Insert

   alpha-synuclein E46K (SNCA Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   E46K

   Promoter

   CMV

   Available since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pT7-7 asyn E46K

   Plasmid

   #105728

   Purpose

   Bacterial expression of mutant human alpha synuclein

   Depositor

   Hilal Lashuel

   Article

   Mbefo et al J Biol Chem. 2015 Apr 10;290(15):9412-27

   Insert

   alpha-synuclein E46K (SNCA Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   E46K

   Promoter

   T7

   Available since

   April 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA6 asyn A30P

   Plasmid

   #105729

   Purpose

   Mammalian expression of mutant human alpha synuclein

   Depositor

   Hilal Lashuel

   Article

   Mbefo et al J Biol Chem. 2015 Apr 10;290(15):9412-27

   Insert

   alpha-synuclein A30P (SNCA Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   A30P

   Promoter

   CMV

   Available since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLVNP3.0-PEmax

   Plasmid

   #206883

   Purpose

   Expresses FLAG-tagged PEmax fused to Gag through a linker sequence

   Depositor

   Jacob Giehm Mikkelsen

   Article

   Haldrup et al Nucleic Acids Res. 2023 Sep 8:gkad676. d

   Insert

   PEmax
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Oct. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. GFP-DroshaS300/302A

   Plasmid

   #62530

   Purpose

   Serines 300 and 302 of Drosha are mutated to alanines

   Depositor

   Bharat Ramratnam

   Article

   Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

   Insert

   human Drosha with serines 300 and 302 changed alanines (DROSHA Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   changed serines 300 and 302 to alanines

   Promoter

   Available since

   March 20, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pKH3-TRIM28-C65A/C68A

    Plasmid

    #92199

    Purpose

    Mammalian overexpression vector for TRIM28; catalytically inactive (mutated on C65A and C68A)

    Depositor

    Huda Zoghbi

    Article

    Rousseaux et al Elife. 2016 Oct 25;5. pii: e19809. doi:

    Insert

    Tif1b (TRIM28 Synthetic, Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Changed Cysteines 65 and 68 to Alanines.

    Promoter

    Available since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLVNP3.0-ABE8e

    Plasmid

    #206882

    Purpose

    Expresses FLAG-tagged ABE8e fused to Gag through a linker sequence

    Depositor

    Jacob Giehm Mikkelsen

    Article

    Haldrup et al Nucleic Acids Res. 2023 Sep 8:gkad676. d

    Insert

    ABE8e
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Oct. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLVNP3.0-PEmaxRH

    Plasmid

    #206884

    Purpose

    Expresses FLAG-tagged PEmax (lacking the RNAseH domain) fused to Gag through a linker sequence

    Depositor

    Jacob Giehm Mikkelsen

    Article

    Haldrup et al Nucleic Acids Res. 2023 Sep 8:gkad676. d

    Insert

    PEMax Delta RNAseH
    

    Use

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Oct. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHis-LMW_FGF2

    Plasmid

    #157657

    Purpose

    Expresses FGF2 in E.coli cell

    Depositor

    Byung Il Lee

    Article

    Bong et al Nucleic Acids Res. 2020 Jun 19;48(11):63

    Insert

    Fibroblast growth factor 2 (FGF2 Human)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    changed Cystein 211,229 to serine, deleted amino …

    Promoter

    Available since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV14_NHP2L1(15.5kD)

    Plasmid

    #73065

    Purpose

    expression clone for human NHP2L1 (15.5kD) with C-terminal 3xFLAG-tag

    Depositor

    Stefan Stamm

    Article

    Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

    Insert

    NHP2L1 (15.5kD) (SNU13 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV Flag dPDZ-PTEN (1-400)

    Plasmid

    #22232

    Depositor

    Hong Wu

    Article

    Mosessian et al J Biol Chem. 2009 Oct 30;284(44):30159-6

    Insert

    PTEN (Pten Mouse)
    

    Use

    Tags

    Flag

    Expression

    Bacterial and Mammalian

    Mutation

    PDZ binding domain deleted. Contains amino acids …

    Promoter

    Available since

    March 19, 2010

    Availability

    Academic Institutions and Nonprofits only

16. pT7-EGFP-C1-HsNot1iso1-V1251R_R

    Plasmid

    #147376

    Purpose

    Mammalian Expression of HsNot1iso1-V1251R

    Depositor

    Elisa Izaurralde

    Article

    Petit et al Nucleic Acids Res. 2012 Nov;40(21):11058

    Insert

    HsNot1iso1-V1251R (CNOT1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A deletion of 5 AA 822-827 SKMKPS-> T and one …

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. GFP-Drosha1-390

    Plasmid

    #62521

    Purpose

    C-terminal aa391-1374 of Drosha is deleted

    Depositor

    Bharat Ramratnam

    Article

    Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

    Insert

    human Drosha with aa 391-1374 deleted (DROSHA Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    deleted amino acids 391-1374

    Promoter

    Available since

    March 23, 2015

    Availability

    Academic Institutions and Nonprofits only

18. GFP-Drosha1-850

    Plasmid

    #62522

    Purpose

    C-terminal aa851-1374 of Drosha is deleted

    Depositor

    Bharat Ramratnam

    Article

    Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

    Insert

    human Drosha with aa 851-1374 deleted (DROSHA Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    deleted amino acids 851-1374

    Promoter

    Available since

    March 20, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pMT-ubb-NLS-BirA-2a-mCherry

    Plasmid

    #79886

    Purpose

    Ubiquitin promoter driving HA-tagged biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein; flanked by Tol2 sequences

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
    

    Use

    Tags

    3x HA

    Expression

    Bacterial

    Mutation

    Promoter

    Ubiquitin

    Available since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCVL.SSA TLR (CCR5 TALEN Sce Spacer)

    Plasmid

    #46941

    Purpose

    Codes for the SSA-TLR with the target site for the CCR5 TALEN with the spacer region corresponding to I-Sce I in a lentiviral backbone

    Depositor

    Andrew Scharenberg

    Article

    Kuhar et al Nucleic Acids Res. 2013 Oct 10.

    Inserts

    5' iRFP arm
    eGFP with TALEN TS
    +3 mCherry
    3' iRFP arm
    

    Use

    Lentiviral

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    embedded TALEN TS from 163-218, truncated 25 amin…

    Promoter

    SFFV

    Available since

    Nov. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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