We narrowed to 5,091 results for: codon optimized

1. pBHM2642

   Plasmid

   #229526

   Purpose

   Carries OsTIR1-t2a-mNG-mIAA7_opt, used to insert TIR1 expressing construct into the C. neoformans genome.

   Depositor

   Hiten Madhani

   Insert

   OsTIR1
   

   Use

   Tags

   t2a-mNeonGreen-mIAA7_opt

   Expression

   Bacterial and Yeast

   Mutation

   F74G

   Promoter

   Available since

   March 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. ecALDH

   Plasmid

   #67921

   Purpose

   Expresses aldehyde dehydrogenase from Escherichia coli, codon optimized for expression in E. coli

   Depositor

   Justin Siegel

   Insert

   Escherichia coli aldehyde dehydrogenase (puuC Escherichia coli)
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJJR81

   Plasmid

   #75029

   Purpose

   tagBFP2^SEC^3xFlag vector with ccdB markers for cloning homology arms

   Depositor

   Mike Boxem

   Insert

   tagBFP2^SEC^3xFlag
   

   Use

   CRISPR

   Tags

   3xFLAG and C. elegans codon optimized tagBFP2

   Expression

   Worm

   Mutation

   Promoter

   Available since

   June 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET-29b(+)_NSP4ss_SerM_NucA-myc

   Plasmid

   #218191

   Purpose

   Bacterial expression of a periplasmically expressed myc-tagged Serratia Marcescens NucA (Benzonase)

   Depositor

   Florence Pojer

   Insert

   nucA
   

   Use

   Tags

   NSP4 signal secretion peptide and myc

   Expression

   Bacterial

   Mutation

   codon optimized ecoli residues - 22-266

   Promoter

   T7

   Available since

   May 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-CAG-mNeonGreen

   Plasmid

   #124101

   Purpose

   Expression of the fluorescent protein mNeonGreen in mammalian cells

   Depositor

   Pantelis Tsoulfas

   Insert

   mNeonGreen
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Codon usage was optimized for Homo Sapiens

   Promoter

   CAG

   Available since

   April 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBS-L1PA1CH blast

   Plasmid

   #69612

   Purpose

   Expresses a blasticidin tagged codon optimized L1PA1

   Depositor

   Astrid Roy-Engel

   Insert

   L1PA1
   

   Use

   Tags

   blast cassette

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBS-L1PA13ACHmneo

   Plasmid

   #69613

   Purpose

   Expresses a neomycin tagged codon optimized L1PA13A

   Depositor

   Astrid Roy-Engel

   Insert

   L1PA13A
   

   Use

   Tags

   mneo retrotransposition cassette

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCR182

   Plasmid

   #240642

   Purpose

   Template for in vitro transcription of AmNeonGreen mRNA using SP6 polymerase.

   Depositor

   John Pringle

   Insert

   AmNeonGreen
   

   Use

   Vertebrate gene expression and general in vitro t…

   Tags

   Expression

   Mutation

   Codon-optimized for Exaiptasia diaphana, amino ac…

   Promoter

   SP6

   Available since

   Aug. 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBHM2643

   Plasmid

   #229527

   Purpose

   Carries OsTIR1-t2a-mNG-mAID_opt, used to insert TIR1 expressing construct into the C. neoformans genome.

   Depositor

   Hiten Madhani

   Insert

   OsTIR1
   

   Use

   Tags

   t2a-mNeonGreen-mAID_opt

   Expression

   Bacterial and Yeast

   Mutation

   F74G

   Promoter

   Available since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHH0103 STXBP4 WW domain

    Plasmid

    #104265

    Purpose

    Bacterial expression of WW domain from STXBP4

    Depositor

    Sachdev Sidhu, Marius Sudol

    Insert

    STXBP4 WW domain (STXBP4 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    codon optimized for expression in bacteria

    Promoter

    tac

    Available since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHH0103 PIN1L WW domain

    Plasmid

    #104269

    Purpose

    Bacterial expression of WW domain from PIN1L

    Depositor

    Sachdev Sidhu, Marius Sudol

    Insert

    PIN1L WW domain (PIN1P1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    codon optimized for expression in bacteria

    Promoter

    tac

    Available since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTPGI_dCas9_VP64

    Plasmid

    #49013

    Purpose

    encodes yeast-optimized dCas9_VP64 synthetic transcription factor

    Depositor

    Timothy Lu

    Article

    Farzadfard et al ACS Synth Biol. 2013 Sep 11.

    Insert

    dCas9_VP64 (codon-optimized for expression in S. cerevisiae)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    SV40 NLS

    Expression

    Yeast

    Mutation

    Promoter

    pTPGI (galactose+aTc inducible)

    Available since

    Jan. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV_dCas9_VP64

    Plasmid

    #49015

    Purpose

    encodes human-optimized dCas9_VP64 synthetic transcription factor

    Depositor

    Timothy Lu

    Article

    Farzadfard et al ACS Synth Biol. 2013 Sep 11.

    Insert

    dCas9_VP64 (human-codon-optimized)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGC4780

    Plasmid

    #113408

    Purpose

    Translation reporter with inducible translation coupling (high native GFP expression)

    Depositor

    Adam Arkin, Guillaume Cambray

    Article

    Cambray et al Nat Biotechnol. 2018 Nov;36(10):1005-101

    Insert

    158_33121123_1
    

    Use

    Synthetic Biology

    Tags

    fused to codon deptimized sfGFP with Flexible lin…

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGC4785

    Plasmid

    #113409

    Purpose

    Translation reporter with inducible translation coupling (low native GFP expression, high under coupling)

    Depositor

    Adam Arkin, Guillaume Cambray

    Article

    Cambray et al Nat Biotechnol. 2018 Nov;36(10):1005-101

    Insert

    359_31121113_2
    

    Use

    Synthetic Biology

    Tags

    fused to codon deptimized sfGFP with Flexible lin…

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Keasling-2337

    Plasmid

    #100168

    Purpose

    Used to improve production from engineered biosynthetic pathways include optimizing codon usage, enhancing production of rate-limiting enzymes, and eliminating the accumulation of toxic intermediates or byproducts to improve cell growth

    Depositor

    Jay Keasling

    Article

    Anthony et al Metab Eng. 2009 Jan;11(1):13-9. doi: 10.

    Insert

    lacIq-Ptrc-ADS
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    trc

    Available since

    Sept. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. (565) pAAV Alb-AAT KRAB-SadCas9 U6-gSTOP

    Plasmid

    #163030

    Purpose

    Expression of CRISPRi with gSTOP gRNA

    Depositor

    Tonia Rex

    Article

    Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

    Insert

    Mammalian codon-optimized SadCas9
    

    Use

    AAV

    Tags

    KRAB, SV40 pA, and myc NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Alb-AAT

    Available since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. (364) SB KRAB-SadCas9

    Plasmid

    #163022

    Purpose

    Sleeping Beauty TET-On expression of CRISPRi (without U6-gRNA)

    Depositor

    Tonia Rex

    Article

    Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

    Insert

    Mammalian codon-optimized SadCas9
    

    Use

    Transposon

    Tags

    KRAB and myc NLS

    Expression

    Mammalian

    Mutation

    Promoter

    TET-On

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBM019

    Plasmid

    #128179

    Purpose

    Construct for generating stable Cas9-expressing Toxoplasma gondii

    Depositor

    Sebastian Lourido

    Article

    Markus et al mSphere. 2019 Jun 26;4(3). pii: 4/3/e003

    Inserts

    sgRNA#1
    SpCas9
    Chloramphenicol acetyltransferase
    

    Use

    CRISPR; Toxoplasma gondii expression

    Tags

    3X FLAG and Nuclear Localization Signal

    Expression

    Mutation

    Promoter

    TUB1 (Toxoplasma gondii), U6 (Toxoplasma gondii),…

    Available since

    July 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pX330-Flag-dSpCas9

    Plasmid

    #92113

    Purpose

    Expression plasmid for human codon-optimized dead/inactive SpCas9 (without U6-sgRNA coding sequence)

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Insert

    dead/inactive SpCas9 with FLAG tag
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    Cbh

    Available since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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