We narrowed to 3,816 results for: golden gate

1. C20m

   Plasmid

   #120961

   Purpose

   MoClo golden gate assembly CD part with sfYFP-ssrA (superfolder YFP with ssrA degradation tag; variant of https://www.fpbase.org/protein/yfp/)

   Depositor

   Richard Murray

   Insert

   sfYFP-ssrA (has BbsI site)
   

   Use

   Synthetic Biology

   Tags

   ssrA degradation tag

   Available Since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. C21m

   Plasmid

   #120962

   Purpose

   MoClo golden gate assembly CD part for MG-aptamer (fluorescent RNA aptamer of malachite green; see DOI: 10.1021/ja0529788) - Please see Supplemental Documents for annotated Genbank file

   Depositor

   Richard Murray

   Insert

   MG-apt
   

   Use

   Synthetic Biology

   Available Since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. C22m

   Plasmid

   #120963

   Purpose

   MoClo golden gate assembly CD part for mSpinach (fluorescent RNA aptamer for DFHBI, suitable for in vivo use; see DOI: 10.1021/sb400089c). Please see Supplemental Documents for annotated Genbank file.

   Depositor

   Richard Murray

   Insert

   mSpinach
   

   Use

   Synthetic Biology

   Available Since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. P37m

   Plasmid

   #120947

   Purpose

   MoClo golden gate assembly AB part for pRpa* (improved pRpa from DOI: 10.1021/acssynbio.5b00286). Please see Supplemental Documents for annotated Genbank file.

   Depositor

   Richard Murray

   Insert

   pRpa* from Scott and Hasty 2016
   

   Use

   Synthetic Biology

   Available Since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. P38m

   Plasmid

   #120948

   Purpose

   MoClo golden gate assembly AB part for pTra* (improved pTra from DOI: 10.1021/acssynbio.5b00286). Please see Supplemental Documents for annotated Genbank file.

   Depositor

   Richard Murray

   Insert

   pTra* from Scott and Hasty 2016
   

   Use

   Synthetic Biology

   Available Since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. P58m

   Plasmid

   #120950

   Purpose

   MoClo golden gate assembly AB for pLas-A-10b (Adam Meyer's improved pLas promoter from https://doi.org/10.1101/285866, selected for dynamic range and less cross-sensitivity).

   Depositor

   Richard Murray

   Insert

   pLas Adam Meyer
   

   Use

   Synthetic Biology

   Available Since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. P59m

   Plasmid

   #120951

   Purpose

   MoClo golden gate assembly AB part for pLux76B (Adam Meyer's improved pLux promoter from https://doi.org/10.1101/285866, selected for dynamic range and less cross-sensitivity).

   Depositor

   Richard Murray

   Insert

   pLux Adam Meyer
   

   Use

   Synthetic Biology

   Available Since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. p3m

   Plasmid

   #120935

   Purpose

   MoClo golden gate assembly AB part for J23150 (strength ~400x Anderson promoter; see http://parts.igem.org/Promoters/Catalog/Anderson) - Please see Supplemental Documents for annotated Genbank file

   Depositor

   Richard Murray

   Insert

   J23150
   

   Use

   Synthetic Biology

   Available Since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. p4m

   Plasmid

   #120936

   Purpose

   MoClo golden gate assembly AB part for J23151 (strength ~900x Anderson promoter; see http://parts.igem.org/Promoters/Catalog/Anderson) - Please see Supplemental Documents for annotated Genbank file

   Depositor

   Richard Murray

   Insert

   J23151
   

   Use

   Synthetic Biology

   Available Since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAIP4_9C74

    Plasmid

    #234238

    Purpose

    Heterologous protein expression of variant of super folded GFP in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    9C74
    

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pR6K-GFP-RFP

    Plasmid

    #199652

    Purpose

    R6K backbone with Golden Gate (GG) cloning sites

    Depositor

    IIya Finkelstein

    Type

    Empty backbone

    Expression

    Bacterial

    Promoter

    J23119 promoter

    Available Since

    May 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. U25m

    Plasmid

    #121027

    Purpose

    MoClo golden gate assembly BC part for RiboJ-UTR1 (very strong RBS, prefixed by self-cleaving RiboJ for upstream context independence). Please see Supplemental Documents for annotated Genbank file.

    Depositor

    Richard Murray

    Insert

    RiboJ + UTR1
    

    Use

    Synthetic Biology

    Available Since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. T16m

    Plasmid

    #121035

    Purpose

    MoClo golden gate assembly DE part for L3S2P55 (256x-strength synthetic terminator; see DOI: 10.1038/nmeth.2515). Please see Supplemental Documents for annotated Genbank file.

    Depositor

    Richard Murray

    Insert

    L3S2P55 (synthetic Voigt terminator)
    

    Use

    Synthetic Biology

    Available Since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. T17m

    Plasmid

    #121036

    Purpose

    MoClo golden gate assembly DE part L3S2P11 (262x-strength synthetic terminator; see DOI: 10.1038/nmeth.2515). Please see Supplemental Documents for annotated Genbank file.

    Depositor

    Richard Murray

    Insert

    L3S2P11 (synthetic Voigt terminator)
    

    Use

    Synthetic Biology

    Available Since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. T12m

    Plasmid

    #121031

    Purpose

    MoClo golden gate assembly DE part ECK120029600 (378x-strength natural terminator; see DOI: 10.1038/nmeth.2515). Please see Supplemental Documents for annotated Genbank file.

    Depositor

    Richard Murray

    Insert

    ECK120029600 (strongest natural Voigt terminator)
    

    Use

    Synthetic Biology

    Available Since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKS1603

    Plasmid

    #239527

    Purpose

    Express CpPT1(52-408) membrane enzyme cargo and inactive Pex22 peroxisomal chauffeur; chromosomal integration by CRISPR

    Depositor

    John Dueber

    Insert

    2xCrk_SH3-mTurquoise2-CpPT1(52-408)
    

    Expression

    Yeast

    Available Since

    July 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKS1616

    Plasmid

    #239547

    Purpose

    Express GAL-inducible G8H cytochrome P450 and associated reductase as membrane protein cargoes and inactive Pex22 peroxisomal chauffeur; chromosomal integration by CRISPR

    Depositor

    John Dueber

    Insert

    CrG8H-2xCrk_SH3
    

    Expression

    Yeast

    Available Since

    July 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pKS1615

    Plasmid

    #239546

    Purpose

    Express GAL-inducible G8H cytochrome P450 and associated reductase as membrane protein cargoes and Pex22 peroxisomal chauffeur; chromosomal integration by CRISPR

    Depositor

    John Dueber

    Insert

    CrG8H-2xCrk_SH3
    

    Expression

    Yeast

    Available Since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLII-CRISPR for CKA4

    Plasmid

    #238521

    Purpose

    Plant expression of Cas9 and gRNAs against A. thaliana CK2alpha4

    Depositor

    Michael Hothorn

    Insert

    AtCK2alpha4 (AT2G23070 Mustard Weed)
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    June 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pKS1613

    Plasmid

    #239531

    Purpose

    Express CpPT1 membrane protein cargo fused to PTS1 and Pex22(1-36)-mRuby2; chromosomal integration by CRISPR

    Depositor

    John Dueber

    Insert

    mTurquoise2-CpPT1(52-408)-SKL
    

    Expression

    Yeast

    Available Since

    June 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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