We narrowed to 5,008 results for: puc

1. pBBK18 pCAS-Tyr-[gRNA: 1=FF18] (SplitHygR, AmpR)

   Plasmid

   #179002

   Purpose

   Sp.Cas9 and gRNA yeast expression vector with FF18 gRNA pre-cloned. Selection =SplitHygromycin resistance

   Depositor

   Vincent Martin

   Article

   Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

   Insert

   S. pyogenes Cas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. Bxb1-GA_CAG-EGFP

   Plasmid

   #229799

   Purpose

   Bxb1-GA donor plasmid for integrating a transcription unit for constitutive expression of EGFP

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   EGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. Bxb1-GA Donor_hEF1a

   Plasmid

   #229779

   Purpose

   Bxb1-GA donor plasmid for the cloning of payloads <20 kb with hEF1a promoter for expressing landing pad enrichment marker

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. miniTn7(Gm)PA1/04/03-eyfp-a

   Plasmid

   #111620

   Purpose

   eYFP Fluorescent tagging of bacteria at their Tn7-site

   Depositor

   Tim Tolker-Nielsen

   Article

   Klausen et al Mol Microbiol. 2003 Jun;48(6):1511-24.

   Insert

   PA1/04/03-eyfp
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pX330A_D10A-1x2

   Plasmid

   #58772

   Purpose

   Expresses Cas9 nickase and gRNA

   Depositor

   Takashi Yamamoto

   Article

   Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

   Insert

   humanized S. pyogenes Cas9 (D10A) nickase
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   D10A nickase-converting mutation

   Promoter

   CBh

   Available since

   Nov. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRA1EGFP

   Plasmid

   #193791

   Purpose

   Expresses EGFP under the control of PR promoter, pUC origin of replication, Ampicillin selection

   Depositor

   Sangram Bagh

   Article

   Sarkar et al J Biol Eng. 2019 Mar 1;13:20. doi: 10.11

   Insert

   EGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PR promoter

   Available since

   April 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-SCP1-dSa-VPR mini.

   Plasmid

   #99685

   Purpose

   Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter

   Depositor

   George Church

   Article

   Vora et al bioRxiv 2018. 298620

   Insert

   dCas9
   

   Use

   AAV

   Tags

   VPR mini

   Expression

   Mutation

   dead Cas9

   Promoter

   Available since

   Sept. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. Bxb1-GA Donor_CAG

   Plasmid

   #229780

   Purpose

   Bxb1-GA donor plasmid for the cloning of payloads <20 kb with CAG promoter for expressing landing pad enrichment marker

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Level 1 P4 TaU6 guide acceptor

   Plasmid

   #165600

   Purpose

   GoldenGate (MoClo) Level 1 Position 4 TaU6 guide acceptor plasmid

   Depositor

   John Innes Centre - Wheat Transformation and Genome Editing, Wendy Harwood

   Article

   Smedley et al Curr Protoc. 2021 Mar;1(3):e65. doi: 10.

   Insert

   TaU6 promoter::LacZ::sgRNA scaffold
   

   Use

   CRISPR and Synthetic Biology; Sgrna acceptor with…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Level 1 P3 TaU6 guide acceptor

    Plasmid

    #165599

    Purpose

    GoldenGate (MoClo) Level 1 Position 3 TaU6 guide acceptor plasmid

    Depositor

    John Innes Centre - Wheat Transformation and Genome Editing, Wendy Harwood

    Article

    Smedley et al Curr Protoc. 2021 Mar;1(3):e65. doi: 10.

    Insert

    TaU6 promoter::LacZ::sgRNA scaffold
    

    Use

    CRISPR and Synthetic Biology; Sgrna acceptor with…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pX330A_D10A-1x5

    Plasmid

    #58775

    Purpose

    Expresses Cas9 nickase and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

    Insert

    humanized S. pyogenes Cas9 (D10A) nickase
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    D10A nickase-converting mutation

    Promoter

    CBh

    Available since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. KG#62

    Plasmid

    #110874

    Purpose

    Expresses the C. elegans acy-1 cDNA pan-neuronally

    Depositor

    Kenneth Miller

    Article

    Reynolds et al Genetics. 2005 Feb;169(2):651-70. doi: 1

    Inserts

    rab-3 promoter
    acy-1 cDNA
    unc-54 3' control region with 1 intron just upstream and 1 intron in control region
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMAD7-crRNA-2

    Plasmid

    #170135

    Purpose

    For crRNA expression in monocotyledons protoplasts

    Depositor

    Caixia Gao

    Article

    Lin et al J Genet Genomics. 2021 May 3. pii: S1673

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Sept. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAV-EFS-dSa-VPR mini.

    Plasmid

    #99686

    Purpose

    Expresses dSa Cas9 fused to optimized combination of truncated activation domains from EFS promoter

    Depositor

    George Church

    Article

    Vora et al bioRxiv 2018. 298620

    Insert

    dCas9
    

    Use

    AAV

    Tags

    VPR mini

    Expression

    Mutation

    dead Cas9

    Promoter

    Available since

    Sept. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. miniTn7(Gm)PA1/04/03-ecfp-a

    Plasmid

    #111619

    Purpose

    eCFP Fluorescent tagging of bacteria at their Tn7-site

    Depositor

    Tim Tolker-Nielsen

    Article

    Klausen et al Mol Microbiol. 2003 Jun;48(6):1511-24.

    Insert

    PA1/04/03-ecfp
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. mNeonGreen-P2A-3xFLAG-msEEA1 HDR template

    Plasmid

    #214990

    Purpose

    Endogenously tag mouse EEA1 with an N-terminal 3xFLAG in addition to cytosolic mNG for cell sorting.

    Depositor

    Wade Harper

    Article

    Hollingsworth et al bioRxiv 2024.04.19.590338

    Insert

    EEA1 (Eea1 Mouse)
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    Available since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pX330A_dCas9-1x2

    Plasmid

    #63596

    Purpose

    Expresses dCas9 and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

    Insert

    humanized S. pyogenes dCas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    D1361Y

    Promoter

    CBh

    Available since

    June 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pX330A_D10A-1x7

    Plasmid

    #58777

    Purpose

    Expresses Cas9 nickase and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

    Insert

    humanized S. pyogenes Cas9 (D10A) nickase
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    D10A nickase-converting mutation

    Promoter

    CBh

    Available since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDONRPEX18Km-SceI-pheS

    Plasmid

    #111610

    Purpose

    Gateway compatible allelic exchange vector for Burkholderia sp.

    Depositor

    Tim Tolker-Nielsen

    Article

    Fazli et al Appl Environ Microbiol. 2015 Jun;81(11):

    Type

    Empty backbone

    Use

    Allelic exchange in burkholderia sp.

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pX330A_FokI-1x2

    Plasmid

    #63602

    Purpose

    Expresses FokI-dCas9 and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

    Insert

    FokI-dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    June 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge