We narrowed to 7,660 results for: ski

1. pscALPS gag-gfp/vpx

   Plasmid

   #115808

   Purpose

   SFFV promoter expresses gag-gfp fusion with CypA promoter driving expression of SIVMAC251 vpx

   Depositor

   Jeremy Luban

   Article

   Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

   Insert

   gag-gfp
   

   Use

   Lentiviral

   Promoter

   SFFV

   Available Since

   March 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDH-CRY2-mCh-superPLDx10

   Plasmid

   #188986

   Purpose

   A lentiviral plasmid encoding CRY2-mCh-superPLDx10 (an engineered PLD mutant with 10 times higher activity than the wild-type)

   Depositor

   Jeremy Baskin

   Article

   Tei et al Nat Chem. 2023 May 22. doi: 10.1038/s415

   Insert

   PLD
   

   Tags

   CRY2 and mCherry

   Expression

   Mammalian

   Mutation

   F163L, K249R, G328S, G381V, G429D, D480G

   Promoter

   CMV

   Available Since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAPM-D4 miR30-SUV39h1 ts2

   Plasmid

   #115881

   Purpose

   SUV39h1 knockdown

   Depositor

   Jeremy Luban

   Article

   Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

   Insert

   SUV39h1 shRNA (SUV39H1 Human)
   

   Use

   Lentiviral

   Promoter

   SFFV

   Available Since

   Jan. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMC-J6-NbGP41-sfGFP-VP64-GB1-J9

   Plasmid

   #202007

   Purpose

   MoClo level 0 NbGP41-sfGFP-VP64-GB1 coding sequence module

   Depositor

   Michael Smanski

   Article

   Casas-Mollano et al Nucleic Acids Research, Volume 51, Issue

   Insert

   NbGP41-sfGFP-VP64-GB1
   

   Use

   Synthetic Biology; Moclo cloning vector

   Tags

   GB1 and sfGFP

   Available Since

   July 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAPM-D4 miR30-SUV39h1 ts1

   Plasmid

   #115880

   Purpose

   SUV39h1 knockdown

   Depositor

   Jeremy Luban

   Article

   Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

   Insert

   SUV39h1 shRNA (SUV39H1 Human)
   

   Use

   Lentiviral

   Promoter

   SFFV

   Available Since

   Jan. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLENTI CMV GFP TREX1 R128A R174A BLAST

   Plasmid

   #164234

   Purpose

   lentiviral plasmid for GFP-TREX1 R128A R174A

   Depositor

   John Maciejowski

   Article

   Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

   Insert

   TREX1 (TREX1 Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   R124A R174A

   Promoter

   CMV

   Available Since

   March 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET15b/bPanK

   Plasmid

   #73619

   Purpose

   Produces Escherichia coli pantothenate kinase, with N-terminal His6 tag (H6PanK)

   Depositor

   T. J. Kappock, Suzanne Jackowski

   Article

   Calder et al J Biol Chem. 1999 Jan 22;274(4):2014-20.

   Insert

   pantothenate kinase (coaA Escherichia coli)
   

   Tags

   His6

   Expression

   Bacterial

   Mutation

   relative to MG1655 sequence, changes glutamate-5 …

   Promoter

   T7

   Available Since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pscALPS-HIV-1 Group O-vpr

   Plasmid

   #115836

   Purpose

   Encodes codon optimized 3xFLAG-HIV1 Group O Vpr (L20571) and puromycin resistance protein

   Depositor

   Jeremy Luban

   Article

   Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

   Insert

   HIV1 Group O Vpr (L20571)
   

   Use

   Lentiviral

   Tags

   3x-FLAG

   Promoter

   SFFV

   Available Since

   Jan. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. p1071-Lenti-Single loxP

   Plasmid

   #217892

   Purpose

   Lentiviral vector, entry vector for Lentiviral Switch-OVER

   Depositor

   Ulrich Elling

   Article

   Chylinski et al Nat Commun. 2019 Nov 29;10(1):5454. doi:

   Type

   Empty backbone

   Use

   CRISPR, Cre/Lox, and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-Gad65P-eGFP-3XNLS-WPRE-pA

    Plasmid

    #183804

    Purpose

    AAV vector to express nuclear localized eGFP from a Gad65 promoter

    Depositor

    Jonathan Ploski

    Article

    de Solis et al Brain Res. 2017 Oct 1;1672:148-162. doi:

    Insert

    eGFP-NLS
    

    Use

    AAV

    Tags

    NLS

    Available Since

    May 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDH-CRY2-mCh-superPLDx30

    Plasmid

    #188988

    Purpose

    A lentiviral plasmid encoding CRY2-mCh-superPLDx30 (an engineered PLD mutant with 30 times higher activity than the wild-type)

    Depositor

    Jeremy Baskin

    Article

    Tei et al Nat Chem. 2023 May 22. doi: 10.1038/s415

    Insert

    PLD
    

    Tags

    CRY2 and mCherry

    Expression

    Mammalian

    Mutation

    K109R, P245A, G328S, G381V, G429D

    Available Since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCellFree_G03 GDI2

    Plasmid

    #67093

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    GDI2 (GDI2 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pVG122

    Plasmid

    #211765

    Purpose

    Expresses strongLOV under PGK1pr. StrongLOV is a variant of optogenetic transcription factor EL222 that is more sensitive than EL222 to blue light.

    Depositor

    Sahand Jamal Rahi

    Article

    Gligorovski et al Nat Commun. 2023 Jun 27;14(1):3810. doi:

    Insert

    strongLOV
    

    Use

    For single-copy integration (if cut with pmei)

    Tags

    SV40 NLS and VP16

    Expression

    Yeast

    Mutation

    compared to EL222, strongLOV contains Glu84Asp mu…

    Available Since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. EPB104

    Plasmid

    #68369

    Purpose

    contains human U6 promoter

    Depositor

    Eric-Paul Bennett

    Article

    Lonowski et al Nat Protoc. 2017 Mar;12(3):581-603. doi:

    Insert

    U6 promoter (RNU6-1 Human)
    

    Expression

    Bacterial

    Promoter

    U6

    Available Since

    Feb. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-EF1a-DIO-APEX.NES-P2A-EGFP

    Plasmid

    #182824

    Purpose

    Cre-dependent expression of cytosolic APEX

    Depositor

    Yevgenia Kozorovitskiy

    Article

    Dumrongprechachan et al Nat Commun. 2021 Aug 11;12(1):4855. doi:

    Insert

    APEX.NES-P2A-EGFP
    

    Use

    AAV

    Tags

    EGFP reporter (P2A)

    Promoter

    EF1a

    Available Since

    May 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMOD_D-CmYLCV-NbGP41P-sfGFP-VP64-GB1

    Plasmid

    #202010

    Purpose

    Moclo level 1 - Module D, Promoter: CmYLCV, Gene: NbGP41P-sfGFP-VP64-GB1, Terminator: ZmUBI

    Depositor

    Michael Smanski

    Article

    Casas-Mollano et al Nucleic Acids Research, Volume 51, Issue

    Insert

    NbGP41P-sfGFP-VP64-GB1
    

    Use

    Synthetic Biology; Moclo level 1 vector

    Tags

    GB1 and sfGFP

    Promoter

    CmYLCV

    Available Since

    July 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBetaActin-Halo-CAMSAP3

    Plasmid

    #191329

    Purpose

    Expresses the fluorescently labeled microtubule minus end marker CAMSAP3 to measure microtubule retrograde flow

    Depositor

    Frank Bradke

    Article

    Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

    Insert

    Halo-CAMSAP3 (Camsap3 Mouse)
    

    Expression

    Mammalian

    Available Since

    Jan. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. LNCX BMP-RII

    Plasmid

    #12641

    Depositor

    Rik Derynck

    Article

    Skillington et al J Cell Biol. 2002 Oct 14. 159(1):135-46.

    Insert

    BMP-RII (BMPR2 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Oct. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

19. RhoC FLARE.sc mCer, mVenus - CA

    Plasmid

    #65424

    Purpose

    RhoC CA biosensor single chain; mCerulean-tagged ROCK-RBD, mVenus-tagged RhoC Q63L

    Depositor

    Klaus Hahn

    Article

    Zawistowski et al PLoS One. 2013 Nov 5;8(11):e79877. doi:

    Insert

    RBD, mCerulean, mVenus, RhoC
    

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    T153M in mVenus, Q63L in RhoC

    Available Since

    Aug. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

20. RhoC FLARE.sc mCer, mVenus - DN

    Plasmid

    #65423

    Purpose

    RhoC DN biosensor single chain; mCerulean-tagged ROCK-RBD, mVenus-tagged RhoC T19N

    Depositor

    Klaus Hahn

    Article

    Zawistowski et al PLoS One. 2013 Nov 5;8(11):e79877. doi:

    Insert

    RBD, mCerulean, mVenus, RhoC
    

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    T153M in mVenus, T19N in RhoC

    Available Since

    Aug. 11, 2015

    Availability

    Academic Institutions and Nonprofits only