We narrowed to 3,175 results for: tef

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YIp ADE2-NatMx

Plasmid

#122918

Purpose

ADE2-NatMx integrating (YIp) vector for stable genomic integration in the BY4741 background.

Depositor

Randy Hampton

Publication

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Available since

Oct. 18, 2019

Availability

Academic Institutions and Nonprofits only

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YIp ADE2-HphMx

Plasmid

#122916

Purpose

ADE2-HphMx integrating (YIp) vector for stable genomic integration in the BY4741 background.

Depositor

Randy Hampton

Publication

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Available since

Oct. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pBES2

Plasmid

#83800

Purpose

Useful for tagging yeast proteins with N-terminal Venus tag; G418 selection

Depositor

Eric Muller

Publication

Hailey et al Methods Enzymol. 2002;351:34-49.

Insert

Venus

Expression

Yeast

Available since

Sept. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pKV005

Plasmid

#64763

Purpose

Recombination-Induced Tag Exchange (RITE) Cassette, HA to a T7 epitope tag, invariable Myc tag, Hygro selection

Depositor

Fred van Leeuwen

Publication

Terweij et al G3 (Bethesda). 2013 Aug 7;3(8):1261-72.

Insert

RITE cassette

Use

Rite cassette

Promoter

none

Available since

July 6, 2015

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yomRuby-SpHis5

Plasmid

#44843

Purpose

Yeast tagging vector to create gene fusion with yeast optimized mRuby with SpHis5 selection

Depositor

Wendell Lim, Kurt Thorn

Publication

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yomRuby

Expression

Yeast

Available since

May 10, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pFA6a-mIAA7-ALFA-HIS3MX6

Plasmid

#247627

Purpose

Yeast gene tagging with mIAA7

Depositor

Sebastian Schuck

Publication

Hubbe et al Mol Biol Cell. 2026 Feb 1;37(2):mr2. doi

Insert

mIAA7

Tags

ALFA

Expression

Yeast

Available since

Dec. 12, 2025

Availability

Academic Institutions and Nonprofits only

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UG75-PAH1-3HA

Plasmid

#184761

Purpose

PAH1 overexpression. Promotes the formation of diacylglycerol from phosphatidate. Uses antibiotic resistance marker hphMX6.

Depositor

Zhiping Xie

Publication

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

PAH1 (PAH1 Budding Yeast)

Tags

3HA

Expression

Yeast

Promoter

PAH1

Available since

Feb. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pGAL-PDE1

Plasmid

#160388

Purpose

Overxpression of PDE1 in yeast

Depositor

John McCusker

Insert

PDE1

Expression

Yeast

Promoter

GAL

Available since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pICE LYS2-HphMx

Plasmid

#122898

Purpose

LYS2-HphMx ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

Depositor

Randy Hampton

Publication

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Available since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pICE TRP1-KanMx

Plasmid

#122902

Purpose

TRP1-KanMx ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

Depositor

Randy Hampton

Publication

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Available since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pICE TRP1-NatMx

Plasmid

#122903

Purpose

TRP1-NatMx ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

Depositor

Randy Hampton

Publication

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Available since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pAV0758

Plasmid

#133511

Purpose

Expression of 3GFP tagged RasAct probe to monitor active Ras1

Depositor

Sophie Martin

Publication

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pLys3(BstZ17I)-p(pak1)-RasAct(3xbyr2RBD)-3GFP-kanMX-terminator(ScADH1)

Expression

Yeast

Available since

Nov. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pICE ADE2-NatMx

Plasmid

#122897

Purpose

ADE2-NatMx ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

Depositor

Randy Hampton

Publication

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Available since

Aug. 30, 2019

Availability

Academic Institutions and Nonprofits only

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SZ_EC_02

Plasmid

#155341

Purpose

Overexpression of xylose utilization genes of Yarrowia lipolytica using EasyCloneYALI. Cut with NotI to linearize repair fragment for C3 locus, overexpressing XK, XDH, and XR

Depositor

Jens Nielsen

Publication

Konzock et al BMC Microbiol. 2021 Mar 8;21(1):77. doi:

Inserts

tef1 promoter - xylulose kinase - pex20 terminator
pyk1 promoter - xylose reductase - pex16 terminator
gapdh promoter - xylitol dehydrogenase - lip2 terminator

Use

Easycloneyali insert vector

Expression

Yeast

Promoter

gapdh, pyk1, and tef1

Available since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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pUDE731

Plasmid

#103008

Purpose

S. cerevisiae Episomal plasmid harboring Fncpf1 under the control of TDH3p

Depositor

Jean-Marc Daran

Publication

Swiat et al Nucleic Acids Res. 2017 Dec 1;45(21):125

Insert

Fncpf1

Tags

3HA and NLS

Expression

Yeast

Mutation

human codon optimized

Promoter

TEF1

Available since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCAS9c

Plasmid

#141250

Purpose

Constitutive expression of Cas9 for addressing one target; Contains guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.

Depositor

Adam Bertl

Publication

Degreif et al J Biol Methods. 2018 Sep 12;5(3):e98. do

Type

Empty backbone

Use

CRISPR

Expression

Yeast

Promoter

TEF1 (Cas9), pSNR52 (gRNA)

Available since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pCRISPEY-GAL-Nat

Plasmid

#232097

Purpose

Yeast CEN plasmid for galactose-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with NatMX selection

Depositor

Jeffrey Lewis

Publication

Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

Type

Empty backbone

Use

CRISPR

Expression

Yeast

Available since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pRSII41K-TDH3pr-mNeon

Plasmid

#194534

Purpose

Low copy vector backbone with G418 selectivity, encodes for TDH3(pr) driven expression of mNeon

Depositor

Patrick Gibney

Publication

Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

Insert

kanMX

Expression

Yeast

Promoter

TDH3

Available since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pPath-BGC08-SGE

Plasmid

#200006

Purpose

BGC08 pathway. SGE

Depositor

Farren Isaacs

Publication

Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.

Insert

Pathways

Expression

Bacterial

Available since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pAW8-aid-Turg1:kanMX6

Plasmid

#169354

Purpose

C-terminal aid degron cassette with kanMX6 marker for S. pombe

Depositor

Tony Carr

Insert

auxin-inducible degron (aid)

Expression

Yeast

Available since

May 19, 2021

Availability

Academic Institutions and Nonprofits only

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