We narrowed to 8,824 results for: trac
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Plasmid#51606Purposeexpresses myc-tagged Sema4D/CD4 non-cleavable chimera with extracellular Sema4D, intracellular CD4 in lentiviral backboneDepositorInsertSema4D/CD4-FLAG (Sema4d Mouse, Human)
UseLentiviralTagsFLAG and mycExpressionMammalianMutationSema4D amino acids 660-861 were replaced with CD4…PromoterRSVAvailable sinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMIR-Report-RASA1-3'UTR (mut - miR-206 site)
Plasmid#62576PurposeTranslational Luciferase Reporter containing the mutated 3'UTR of RASA1. The miR-206 binding site was mutated.DepositorInsertRASA1 (RASA1 Human)
UseLuciferaseTagsNoneExpressionMutationmiR-206 binding site mutated (ACATTCCA --> AAC…PromoterAvailable sinceMay 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Flag YFP hNKCC1 cysteineless (NT868)
Plasmid#49079PurposeExpresses human NKCC1 mutant lacking cysteine residues and with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.DepositorInserthuman NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
UseTags3x Flag and mVenusExpressionMammalianMutationC295A, C543M, C563S,C568S,C577S,C582S, C630S, C72…PromoterCMVAvailable sinceNov. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Flag YFP hNKCC1 TM-cysteineless (NT859)
Plasmid#49078PurposeExpresses human NKCC1 mutant lacking cysteine residues within transmembrane domains and with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic,containing convenient restriction sitesDepositorInserthuman NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
UseTags3x Flag and mVenusExpressionMammalianMutationC295A, C543M, C630S, C723S, C724V in hNKCC1 in NT…PromoterCMVAvailable sinceNov. 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBB
Plasmid#53210PurposeContains a 103bp telomeric trackDepositorInsertTTAGGG(n) Track
UseTagsExpressionMutationPromoterT7Available sinceJune 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pVY-18 TC-sfGFP
Plasmid#194632Purposefluorescent protein with tetracysteine tagDepositorInserttetracysteine-sfGFP
UseTagsMGCCPGCCGGAExpressionBacterialMutationPromoterT7Available sinceJan. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
p2B-56
Plasmid#177925PurposeExpresses optimized unleaky reverse tetracycline transactivator (rtTA-M2-SE-G72P) under control of yeast MYO2 promoterDepositorArticleInsertOptimized unleaky reverse tetracycline transactivator (rtTA-M2-SE-G72P) under control of yeast MYO2 promoter
UseTagsExpressionBacterial and YeastMutationV9I; F67S; G72P, F86Y; R171KPromoterMYO2Available sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
p2B-55
Plasmid#177924PurposeExpresses optimized unleaky reverse tetracycline transactivator (rtTA-M2-SE-G72P) under control of yeast TDH3 promoterDepositorArticleInsertOptimized unleaky reverse tetracycline transactivator (rtTA-M2-SE-G72P) under control of yeast TDH3 promoter
UseTagsExpressionBacterial and YeastMutationV9I; F67S; G72P, F86Y; R171KPromoterTDH3Available sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 VDAC1-TC
Plasmid#211733PurposeExpresses tetracysteine tagged VDAC1 in mammalian cells. TC-tag is attached to the C-terminus of VDAC1.DepositorInsertVoltage-dependent anion channel 1 (VDAC1 )
UseTagsTetracysteineExpressionMammalianMutationPromoterCMVAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pVY-16 TC-TEM1
Plasmid#194630Purposeantibiotic resistance enzyme with tetracysteine tag for visualization of enzymeDepositorInserttetracysteine-TEM1
UseTagsMGCCPGCCGGAExpressionBacterialMutationPromoterpBADAvailable sinceJan. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pVY-14 TC-CAT
Plasmid#194628Purposeantibiotic resistance enzyme with tetracysteine tag for visualization of enzymeDepositorInserttetracysteine-CAT
UseTagsMGCCPGCCGGAExpressionBacterialMutationPromoterT7Available sinceJan. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pVY-17 TC-TEM1-tag3
Plasmid#194631Purposeantibiotic resistance enzyme with cationic peptide tag and tetracysteine tagDepositorInserttetracysteine-TEM1-GGSKKRKKRGGSKKRKKRGGSKKRKKR
UseTagsGGSKKRKKRGGSKKRKKRGGSKKRKKR and MGCCPGCCGGAExpressionBacterialMutationPromoterpBADAvailable sinceApril 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pVY-19 TC-sfGFP-tag3
Plasmid#194633Purposefluorescent protein with cationic peptide tag and tetracysteine tagDepositorInserttetracysteine-sfGFP-K(GGSKKRKKR)3
UseTagsKGGSKKRKKRGGSKKRKKRGGSKKRKKR and MGCCPGCCGGAExpressionBacterialMutationPromoterT7Available sinceJan. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pVY-15 TC-CAT-tag3
Plasmid#194629Purposeantibiotic resistance enzyme with cationic peptide tag and tetracysteine tagDepositorInserttetracysteine-CAT-(GGSKKRKKR)3
UseTagsGGSKKRKKRGGSKKRKKRGGSKKRKKR and MGCCPGCCGGAExpressionBacterialMutationPromoterT7Available sinceJan. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pIN1_GGGSx3_mScarlet
Plasmid#182936PurposeC-terminal mScarlet with flexible linker (GGGGS)x3DepositorTypeEmpty backboneUseTagsExpressionWormMutationPromoterAvailable sinceAug. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCS2 Notch1 ICv-6MT
Plasmid#41730DepositorInsertNotch-1 Intracellular Domain (Notch1 Mouse)
UseTags6xMycExpressionMammalianMutationContains murine Notch-1 protein beginning at Vali…PromoterAvailable sinceDec. 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBrain-ch-TOGKDP(L1939A,L1942A)-GFP-shch-TOG
Plasmid#69114PurposeDual-promoter plasmid to express knockdown-proof human ch-TOG with L1939A and L1942A substitutions tagged with EGFP along with shRNA to knock down endogenous ch-TOG in mammalian cells.DepositorInsertsUseRNAiTagsEGFPExpressionMammalianMutationL1939A and L1942A amino acid substitutions. Sile…PromoterCMVAvailable sinceOct. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
PA-DAD-dark
Plasmid#53096PurposeExpresses dark mutant PA-DAD in mammalian cells, an optogenetic tool to activate endogenous diaphanous related formins in live cells using light with LOV2 in the closed conformationDepositorInsertLOV(dark)-6aa-DAD
UseTags6x His and mVenusExpressionBacterial, Insect, and Mamm…MutationL47F and C49S in Venus; C450M mutation in LOV2PromoterAvailable sinceAug. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Flag YFP hNKCC1 N-terminus T,S mutations (NT51)
Plasmid#49061PurposeExpresses human NKCC1 mutated Ser and Thr residues in the N-terminus and an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.DepositorInserthuman NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
UseTags3x Flag and mVenusExpressionMammalianMutationS150N, S155Q, S170Q, T177A, S183R, S193E, T203A, …PromoterCMVAvailable sinceNov. 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
XE129 beta2AR Fz1-pcDNA3.1
Plasmid#16795DepositorUseXenopus expressionTagsExpressionMutationChimera of beta 2 adrenergic receptor (extracellu…PromoterAvailable sinceMarch 13, 2008AvailabilityAcademic Institutions and Nonprofits only