175,634 results
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Plasmid#188778PurposedCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLSDepositorInsertZim3-dCas9
UseLentiviralTagsHA-2xNLS and Zim3 KRAB-NLS fusionPromoterEF1aAvailable SinceOct. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
Laconic/pcDNA3.1(-)
Plasmid#44238Purposea genetically-encoded sensor for lactate concentration, transport and metabolic fluxDepositorInsertLaconic (LACtate Optical Nano Indicator from CECs)
UseLactate biosensorExpressionMammalianPromoterCMVAvailable SinceMay 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
TFORF2315
Plasmid#142030PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
PT7lacO-EF-Tu-His8
Plasmid#224370PurposeExpress EF-Tu protein for his-tagged purificationDepositorAvailable SinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
FRE5
Plasmid#62377PurposeFluorescent reporter to quantify the ratio of two alternative splicing patterns.DepositorInsertdsRed and GFP
ExpressionMammalianAvailable SinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
EcoFlex MoClo Kit
Plasmid Kit#1000000080PurposeCollection of DNA parts for synthetic biology use in E. coli, cell-free protein synthesis, protein purification, and hierarchical assembly of transcription units.DepositorApplicationCloning and Synthetic BiologyVector TypeBacterial ExpressionCloning TypeGolden GateAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40
Plasmid#105540PurposeAAV expression of EGFP-Cre from hSyn promoterDepositorHas ServiceAAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9InsertEGFP-Cre
UseAAV and Cre/LoxExpressionMammalianPromoterhSynAvailable SinceFeb. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
dCas13d-dsRBD
Plasmid#154938PurposeBPNLS-dRfxCas13d-dsRBD-BPNLS-HADepositorInsertBPNLS-dRfxCas13d-dsRBD-BPNLS-HA
UseLentiviralTagsHAExpressionMammalianPromoterCMVAvailable SinceSept. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
HotPETase_pET21b
Plasmid#215407PurposeExpression construct for HotPETase gene, codon optimized for expression in E. coliDepositorInsertHotPETase
ExpressionBacterialMutationS121E/D186H/R280A/P181V/S207R/S214Y/Q119K/S213E/N…Available SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-hChR2(H134R)-EYFP (AAV Retrograde)
Viral Prep#26973-AAVrgPurposeReady-to-use AAV Retrograde particles produced from pAAV-hSyn-hChR2(H134R)-EYFP (#26973). In addition to the viral particles, you will also receive purified pAAV-hSyn-hChR2(H134R)-EYFP plasmid DNA. hSyn-driven, humanized channelrhodopsin H134R mutant fused to EYFP for optogenetic activation. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsEYFPAvailable SinceOct. 9, 2019AvailabilityAcademic Institutions and Nonprofits only