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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,679 results for: αGFP

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Showing: 1241 - 1260 of 1679 results

  1. Nup54 no FG-mEGFP494(2)

    Plasmid
    #164271
    Purpose
    Mammalian expression of Nup54 without the FG-Nup domain, with mEGFP (missing the first 5 amino acids) at amino acid position 494 with a rigid alpha helix of 7 amino acids at the carboxyl end of mEGFP
    Depositor
    Sanford Simon
    Publication
    Pulupa et al Elife. 2020 Dec 21;9. pii: 60654. doi: 1
    Insert
    Nup54 (NUP54 Human)
    Tags
    mEGFP
    Expression
    Mammalian
    Mutation
    deleted the FG region of Nup54
    Promoter
    CMV
    Available since
    Feb. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    164271-plasmid-map-sequence-id-325399

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  2. Nup54 no FG-mEGFP494(1)

    Plasmid
    #164270
    Purpose
    Mammalian expression of Nup54 without the FG-Nup domain, with mEGFP (missing the first 5 amino acids) at amino acid position 494 with a rigid alpha helix of 6 amino acids at the carboxyl end of mEGFP
    Depositor
    Sanford Simon
    Publication
    Pulupa et al Elife. 2020 Dec 21;9. pii: 60654. doi: 1
    Insert
    Nup54 (NUP54 Human)
    Tags
    mEGFP
    Expression
    Mammalian
    Mutation
    deleted the FG region of Nup54
    Promoter
    CMV
    Available since
    Feb. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    164270-plasmid-map-sequence-id-325397

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  3. GFP-CD8a(7A)

    Plasmid
    #162498
    Purpose
    Expresses GFP tagged CD8a mutant in mammalian cells. The seven O-glycosylation sites in its stem region are mutated.
    Depositor
    Lei Lu
    Publication
    Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014
    Insert
    CD8a with a mutated stem region (CD8A Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    The five threonine and two serine residues are mu…
    Available since
    Feb. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    162498-plasmid-map-sequence-id-323489

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  4. Nup54-mEGFP494(2)

    Plasmid
    #163428
    Purpose
    Mammalian expression of Nup54 with a mEGFP (missing the first 5 amino acids) at amino acid position 494 with a rigid alpha helix of 7 amino acids at the carboxyl end of the mEGFP
    Depositor
    Sanford Simon
    Insert
    Nup54 (NUP54 Human)
    Tags
    mEGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Jan. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    163428-plasmid-map-sequence-id-321665

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  8. pSbi-pur-KSR1-CA3-EGFP (C-KSR)

    Plasmid
    #217768
    Purpose
    Fluorescent reporter for ceramide (for stable mammalian expression)
    Depositor
    Paula Nunes-Hasler
    Publication
    Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Kinase suppressor of ras 1 CA3 domain (KSR1 Human)
    Use
    Sleeping beauty transposase-compatible genome ins…
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    CA3 domain aa 317-400 plus vector-based linker LE…
    Promoter
    EF-1alpha promoter
    Available since
    June 10, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    217768-plasmid-map-sequence-id-435794

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  17. pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (AAV2)

    Viral prep
    #112677-AAV2
    Purpose
    Ready-to-use AAV2 particles produced from pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (#112677). In addition to the viral particles, you will also receive purified pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP plasmid DNA. EF1a-driven, Cre-dependent color switch. This AAV directs expression of nuclear mCherry in the absence of Cre. In the presence of Cre, nuclear EGFP (and not mCherry) will be expressed. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Brandon Harvey
    Publication
    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3
    Promoter
    EF1a
    Tags
    mCherry (Cre-negative cells), EGFP (Cre-positive cells)
    Available since
    Jan. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
Showing: 1241 - 1260 of 1679 results