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GFP-mDia1-GBD (1-305 aa) in pEGFPN1

Plasmid

#187280

Purpose

Express EGFP-mDia1 GTPase-binding domain

Depositor

Alpha Yap

Article

Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

Insert

mDia1 (Diaph1 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

GBD (1-305aa)

Promoter

CMV

Available since

Sept. 21, 2022

Availability

Academic Institutions and Nonprofits only

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GFP-Rock1-GBD (840-110 aa) in pEGFPN1

Plasmid

#187279

Purpose

Express EGFP-Rock1 GTPase-binding domain

Depositor

Alpha Yap

Article

Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

Insert

Rock1 (Rock1 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

GBD (84-110aa)

Promoter

CMV

Available since

Sept. 21, 2022

Availability

Academic Institutions and Nonprofits only

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GFP-mROCK2

Plasmid

#101296

Purpose

Mammalian expression of Rock2-GFP

Depositor

Alpha Yap

Article

Priya et al Nat Cell Biol. 2015 Oct;17(10):1282-93.

Insert

Rock2 (Rock2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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GFP-ROCK1

Plasmid

#187296

Purpose

Express GFP ROCK1

Depositor

Alpha Yap

Article

Priya et al Nat Cell Biol. 2015 Oct;17(10):1282-93.

Insert

Rock1 (a.k.a. 1110055K06Rik, Rock-I) (Rock1 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pLL5.0_shRNAHsCavin1_Del4UC1_EGFP

Plasmid

#229691

Purpose

Lentiviral expression of shRNA targeting Cavin1 + reconstitution of zebrafish mutant Cavin1 (Del4UC1)

Depositor

Alpha Yap

Article

Brooks et al Mol Biol Cell. 2023 Nov 1;34(12):ar120.

Inserts

shRNA for hCavin1 (CAVIN1 Human)
mutant cavin1b (Del4UC1) (cavin1b Zebrafish)

Use

Lentiviral

Tags

EGFP

Expression

Mutation

Del4UC1

Promoter

LTR viral promoter

Available since

Jan. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pLL5.0_shRNAHsCavin1_MmCavin1_EGFP

Plasmid

#229689

Purpose

Lentiviral expression of shRNA targeting Cavin1 + reconstitution of mouse Cavin1 (Cavin1 rescue)

Depositor

Alpha Yap

Article

Brooks et al Mol Biol Cell. 2023 Nov 1;34(12):ar120.

Inserts

shRNA for hCavin1 (CAVIN1 Human)
mCavin1 (Cavin1 Mouse)

Use

Lentiviral

Tags

EGFP

Expression

Mutation

Promoter

LTR viral promoter

Available since

Jan. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pLL5.0_shRNAHsCavin1_DrCavin1b_EGFP

Plasmid

#229690

Purpose

Lentiviral expression of shRNA targeting Cavin1 + reconstitution of zebrafish Cavin1 (Cavin1 rescue)

Depositor

Alpha Yap

Article

Brooks et al Mol Biol Cell. 2023 Nov 1;34(12):ar120.

Inserts

shRNA for hCavin1 (CAVIN1 Human)
cavin1b (cavin1b Zebrafish)

Use

Lentiviral

Tags

EGFP

Expression

Mutation

Promoter

LTR viral promoter

Available since

Jan. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pLL5.0_shRNAHsCavin1_EGFP

Plasmid

#229688

Purpose

Lentiviral expression of shRNA targeting Cavin1

Depositor

Alpha Yap

Article

Brooks et al Mol Biol Cell. 2023 Nov 1;34(12):ar120.

Insert

shRNA for hCavin1 (CAVIN1 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mutation

Promoter

LTR viral promoter

Available since

Jan. 29, 2025

Availability

Academic Institutions and Nonprofits only

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GFP-α-cateninA-

Plasmid

#229698

Purpose

Transient expression of GFP-alpha-cateninA- in mammalian cells

Depositor

Alpha Yap

Article

Noordstra et al Dev Cell. 2023 Sep 25;58(18):1748-1763.e

Insert

CTNNA1 (alpha-catenin) Human (CTNNA1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

L785A, I792A, V796A

Promoter

CMV promoter

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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GFP-α-cateninΔβH

Plasmid

#229705

Purpose

Transient expression of GFP-alpha-catenin-delta-beta-H in mammalian cells

Depositor

Alpha Yap

Article

Noordstra et al Dev Cell. 2023 Sep 25;58(18):1748-1763.e

Insert

CTNNA1 (alpha-catenin) Human (CTNNA1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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GFP-α-cateninA+

Plasmid

#229697

Purpose

Transient expression of GFP-alpha-cateninA+ in mammalian cells; enhanced actin binding

Depositor

Alpha Yap

Article

Noordstra et al Dev Cell. 2023 Sep 25;58(18):1748-1763.e

Insert

CTNNA1 (alpha-catenin) Human (CTNNA1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

amino acids 670-673, RAIM--> GSGS

Promoter

CMV promoter

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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GFP-α-cateninWT

Plasmid

#229709

Purpose

Transient expression of GFP-alpha-cateninWT in mammalian cells

Depositor

Alpha Yap

Article

Noordstra et al Dev Cell. 2023 Sep 25;58(18):1748-1763.e

Insert

CTNNA1 (alpha-catenin) Human (CTNNA1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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GFP-AHPH-DM

Plasmid

#71368

Purpose

Location sensor for RhoA-GTP

Depositor

Alpha Yap

Article

Priya et al Nat Cell Biol. 2015 Oct;17(10):1282-93.

Insert

C terminus of Anillin (ANLN Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

A740D and E758K

Promoter

CMV

Available since

Nov. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pEGFP-SRGAP1

Plasmid

#187269

Purpose

Expression of SRGAP1-EGFP

Depositor

Alpha Yap

Article

Liang et al Nat Commun. 2017 Oct 5;8(1):790. doi: 10

Insert

SRGAP1 (SRGAP1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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GFP-α-cateninV-

Plasmid

#229696

Purpose

Transient expression of GFP-alpha-cateninV- in mammalian cells

Depositor

Alpha Yap

Article

Noordstra et al Dev Cell. 2023 Sep 25;58(18):1748-1763.e

Insert

CTNNA1 (alpha-catenin) Human (CTNNA1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

L344P

Promoter

CMV promoter

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pLL5.0-MRLC-DD-GFP

Plasmid

#187260

Purpose

Lentiviral expression of myosin regulatory light chain-DD mutant

Depositor

Alpha Yap

Article

Wee et al J Cell Sci. 2020 Jul 6;133(13). pii: jcs

Insert

MRLC1 (MYL9 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mutation

T18D, S19D

Promoter

5' LTR

Available since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pLL5.0-MRLC-AA-GFP

Plasmid

#187259

Purpose

Lentiviral expression of myosin regulatory light chain-AA mutant

Depositor

Alpha Yap

Article

Wee et al J Cell Sci. 2020 Jul 6;133(13). pii: jcs

Insert

MRLC1 (MYL9 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mutation

T18A,S19A

Promoter

5' LTR

Available since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-α-cateninA+ΔβH

Plasmid

#229704

Purpose

Transient expression of GFP-alpha-cateninA+deltabetaH in mammalian cells

Depositor

Alpha Yap

Article

Noordstra et al Dev Cell. 2023 Sep 25;58(18):1748-1763.e

Insert

CTNNA1 (alpha-catenin) Human (CTNNA1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

amino acids 670-673, RAIM--> GSGS

Promoter

CMV promoter

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLViN-E-cadherin-GFP

Plasmid

#229710

Purpose

Lentiviral expression of E-cadherin-GFPTG in mammalian cells

Depositor

Alpha Yap

Article

Noordstra et al Dev Cell. 2023 Sep 25;58(18):1748-1763.e

Insert

CDH1 E-CADHERIN Human (CDH1 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-mROCK1-delta2

Plasmid

#101290

Purpose

Mammalian expression of Rock1-GFP for the study of localisation signaling.

Depositor

Alpha Yap

Article

Priya et al Nat Cell Biol. 2015 Oct;17(10):1282-93.

Insert

Rock1 (Rock1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

1-727 aa

Promoter

CMV

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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