Addgene

We narrowed to 4,220 results for: 256

Showing: 1241 - 1260 of 4220 results

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pCMV5-ALK2 Q207D

Plasmid

#11740

Depositor

Jeff Wrana

Article

Macias-Silva et al J Biol Chem. 1998 Oct 2. 273(40):25628-3

Insert

ALK2 (ACVR1 Human)

Use

Tags

Expression

Mammalian

Mutation

Q207D: activated receptor

Promoter

Available since

Aug. 4, 2006

Availability

Industry, Academic Institutions, and Nonprofits
Cdk2-DN

Plasmid

#1882

Depositor

Sander van den Heuvel

Article

van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

Insert

Cdk2 (CDK2 Human)

Use

Tags

Expression

Mammalian

Mutation

D146N, dominant negative

Promoter

Available since

April 4, 2006

Availability

Academic Institutions and Nonprofits only
c3GIC9

Plasmid

#62191

Purpose

col1a1 targeting vector for inducible [TRE3G]-GFP-IRES-Cas9 expression. Contains NsiI cloning site for U6-sgRNA cassettes

Depositor

Lukas Dow

Article

Dow et al Nat Biotechnol. 2015 Apr;33(4):390-4. do

Insert

humanized S. Pyogenes Cas9

Use

CRISPR

Tags

3x FLAG

Expression

Mutation

Promoter

TRE3G

Available since

Aug. 10, 2016

Availability

Academic Institutions and Nonprofits only

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Cav-GA

Plasmid

#209880

Purpose

Dimerization dependent fluorescent protein GA anchored to cytosolic face of the caveolae with mouse CAVIN1

Depositor

Sarah Cohen

Article

Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

Insert

ddGFP A

Use

Tags

CAVIN1

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pLPO5

Plasmid

#209987

Purpose

Contains Level 0* Part: replication origin (256rep) for the construction of Level 2 plasmids

Depositor

Thorsten Mascher

Article

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Insert

replication origin (256_rep)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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Cav-RA

Plasmid

#209881

Purpose

Dimerization dependent fluorescent protein RA anchored to cytosolic face of the caveolae with mouse CAVIN1

Depositor

Sarah Cohen

Article

Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

Insert

ddRFP A

Use

Tags

CAVIN1

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 26, 2024

Availability

Academic Institutions and Nonprofits only

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pAcGFP1-N1-MAHS

Plasmid

#90034

Purpose

To express GFP-tagged MAHS in mammalian cells transiently

Depositor

Takekazu Kunieda

Article

Tanaka et al PLoS One. 2015 Feb 12;10(2):e0118272. eC

Insert

MAHS

Use

Tags

AcGFP1

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 24, 2017

Availability

Academic Institutions and Nonprofits only

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pET26b-Granzyme A (S-A)

Plasmid

#8825

Depositor

Judy Lieberman

Article

Beresford et al Proc Natl Acad Sci U S A 1997 Aug 19;94(

Insert

Human Granzyme A inactive (GZMA Human)

Use

Tags

(His)6

Expression

Bacterial

Mutation

changed Serine 184 to Alanine

Promoter

Available since

June 22, 2006

Availability

Academic Institutions and Nonprofits only
pFasBac+GFP-CAMSAP3

Plasmid

#59038

Purpose

for baculovirus expression of mouse CAMSAP3 in Sf9 cells

Depositor

Ron Vale

Article

Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

Insert

CAMSAP3 (Camsap3 Mouse)

Use

Tags

His and eGFP

Expression

Insect

Mutation

Promoter

Available since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only

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Cry2(1-531)-mCh-BAXS184E

Plasmid

#117238

Purpose

Light activated recruitment of BAX in mammalian cells with Cry2/CIB optogenetic switch

Depositor

Robert Hughes

Article

Godwin et al J Biol Chem. 2019 Nov 8;294(45):16918-16

Insert

Cry2531-mCh-BAXS184E

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pP2A_NLS_sfGFP

Plasmid

#63709

Purpose

Expressing P2A_NLS_sfGFP with homologous arm of Nanog.

Depositor

Stanley Qi

Article

Yu et al Cell Stem Cell. 2015 Feb 5;16(2):142-7.

Insert

Nanog homologous arm, p2A, NLS, sfGFP, Nanog homologous arm

Use

Just a donor dna with nanog homologous arm for kn…

Tags

sfGFP

Expression

Mutation

Promoter

Available since

April 27, 2015

Availability

Academic Institutions and Nonprofits only

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FLAG-Vti1b

Plasmid

#45914

Depositor

Noboru Mizushima

Article

Itakura et al Cell. 2012 Dec 7;151(6):1256-69. doi: 10

Insert

vesicle transport through interaction with t-SNAREs 1B (VTI1B Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3.1 HA-rnSOX9

Plasmid

#62972

Purpose

Expresses HA-tagged rat SOX9

Depositor

Michael Ruppert

Article

Deng et al J Cell Sci. 2015 Jan 27. pii: jcs.162164

Insert

SOX9 (Sox9 Rat)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

May 28, 2015

Availability

Academic Institutions and Nonprofits only
MARCKS(1-40)-paGFP

Plasmid

#18695

Depositor

Karel Svoboda

Article

Harvey et al Science. 2008 Jul 4. 321(5885):136-40.

Insert

MARCKS(1-40) (MARCKS Human)

Use

Tags

photoactivatable GFP

Expression

Mammalian

Mutation

A206K mutation in paGFP

Promoter

Available since

July 2, 2008

Availability

Academic Institutions and Nonprofits only

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pAAV-hSYNCMV tAKARα

Plasmid

#119921

Purpose

AAV construct with tAKARα being expressed under the human synapsin promotor with WPRE enhancer.

Depositor

Haining Zhong

Article

Ma et al Neuron. 2018 Aug 22;99(4):665-679.e5. do

Insert

tAKARα

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

April 23, 2019

Availability

Academic Institutions and Nonprofits only

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LD-RA

Plasmid

#209863

Purpose

Dimerization dependent fluorescent protein RA anchored to cytosolic face of the lipid droplet membrane with hairpin domain of human GPAT4

Depositor

Sarah Cohen

Article

Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

Insert

ddRFP A

Use

Tags

Hairpin domain of GPAT4 MNFQYISLRLTVLWGLGVLIRYCFL…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pMRXIP GFP-VAMP8

Plasmid

#45919

Depositor

Noboru Mizushima

Article

Itakura et al Cell. 2012 Dec 7;151(6):1256-69. doi: 10

Insert

vesicle-associated membrane protein 8 (VAMP8 Human)

Use

Retroviral

Tags

GFP

Expression

Mutation

Promoter

CMV

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only
Gi-c001

Plasmid

#173078

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

GNAI1 (GNAI1 Human)

Use

Tags

mCitrine

Expression

Bacterial

Mutation

codon optimized

Promoter

Available since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pRK5_tAKARα

Plasmid

#119913

Purpose

Mammalian expression of the tAKARα sensor for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)

Depositor

Haining Zhong

Article

Ma et al Neuron. 2018 Aug 22;99(4):665-679.e5. do

Insert

tAKARα

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 9, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMRXIP GFP-VAMP7

Plasmid

#45920

Depositor

Noboru Mizushima

Article

Itakura et al Cell. 2012 Dec 7;151(6):1256-69. doi: 10

Insert

vesicle-associated membrane protein 7 (Vamp7 Mouse)

Use

Retroviral

Tags

GFP

Expression

Mutation

Promoter

CMV

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only