Addgene

We narrowed to 1,457 results for: Flp

Showing: 1241 - 1260 of 1457 results

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pOC4 Kcnn2-smFP-myc KI

Plasmid

#183438

Purpose

FlpON knock-in for SK2-smFP-myc (amino acid position: T571)

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

gRNA and smFP myc donor

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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pOC5 smFP-HA-Cacna1e KI

Plasmid

#183441

Purpose

FlpOFF knock-in for smFP-HA-CaV2.3 (amino acid position: G5)

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

gRNA and smFP HA donor

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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pOC4 Grin1-smFP-V5 KI

Plasmid

#183439

Purpose

FlpON knock-in for GluN1-smFP-V5 (amino acid position: A20)

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

gRNA and smFP V5 donor

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO/TARG1

Plasmid

#172580

Purpose

For generation of FlpIn lines with untagged TARG1

Depositor

Karla Feijs

Article

Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

Insert

TARG1 (OARD1 Human)

Use

Tags

none

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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AX44_pS.Donor.S1

Plasmid

#100289

Purpose

AAVS1-targeting plasmid

Depositor

Manuel Goncalves

Article

Chen et al Nat Commun. 2017 Sep 22;8(1):657. doi: 1

Insert

18 bp RFLP (MluI+KspAI)

Use

CRISPR and Synthetic Biology; Human targeting

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α-FRT-FLEX-GtACR2-EYFP

Plasmid

#114369

Purpose

expresses Flpo recombinase-dependent GtACR2

Depositor

Mingshan Xue

Article

Messier et al Elife. 2018 Aug 9;7. pii: 38506. doi: 10

Insert

GtACR2-EYFP (NEWENTRY )

Use

AAV

Tags

EYFP

Expression

Mammalian

Mutation

Promoter

Ef1a

Available since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α-FRT-FLEX-GtACR1-EYFP

Plasmid

#114370

Purpose

expresses Flpo recombinase-dependent GtACR1

Depositor

Mingshan Xue

Article

Messier et al Elife. 2018 Aug 9;7. pii: 38506. doi: 10

Insert

GtACR1-EYFP (NEWENTRY )

Use

AAV

Tags

EYFP

Expression

Mammalian

Mutation

Promoter

Ef1a

Available since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5-MIS12-LAP-BUB1

Plasmid

#115904

Purpose

Used for expression of siRNA-resistant MIS12-BUB1 from the FRT site of FlpIn cells upon addition of doxycyclin.

Depositor

Geert Kops

Article

Vleugel et al J Cell Sci. 2015 Aug 15;128(16):2975-82.

Insert

MIS12-BUB1 (MIS12 Human)

Use

Tags

EYFP

Expression

Mammalian

Mutation

BUB1 fused to MIS12

Promoter

Available since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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CMVTO-RBDK65E-nTEVp

Plasmid

#179666

Purpose

Ras Binding Domain split-nTEVP

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

CRaf RBD protein (RAF1 Human)

Use

Tags

nTEVP

Expression

Mammalian

Mutation

K65E

Promoter

Available since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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CMVTO-RBDK65E-cTEVp

Plasmid

#179667

Purpose

Ras Binding Domain split-cTEVP

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

CRaf RBD protein (RAF1 Human)

Use

Tags

cTEVP

Expression

Mammalian

Mutation

K65E

Promoter

Available since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5-hygro-eGFP-HEC1-8A/S55D

Plasmid

#114032

Purpose

Inducible expression of eGFP-tagged HEC1-8A/S55D in FLPin Trex cell lines

Depositor

Geert Kops

Article

Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1

Insert

eGFP-HEC1-8A/S55D (NDC80) (NDC80 Human)

Use

Tags

Expression

Mammalian

Mutation

Hec1 - 8A/S55D

Promoter

Available since

Sept. 6, 2018

Availability

Academic Institutions and Nonprofits only

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CA-0013 CMVTO-SumIL2-rens-IL2Rb

Plasmid

#231195

Purpose

CageIL-2

Depositor

Xiaojing Gao

Article

Aldrete et al Nat Chem Biol. 2025 Jan 15. doi: 10.1038

Insert

SumIL2-rens-IL2Rb (IL2 Human)

Use

Tags

Expression

Mammalian

Mutation

L80F, R81D, L85V, I86V, I92F

Promoter

Available since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

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CA-0192 CMVTO-Renin(mouse)-ECL-CD4-tevs-RXR6.2

Plasmid

#231204

Purpose

ER-retained mouse renin

Depositor

Xiaojing Gao

Article

Aldrete et al Nat Chem Biol. 2025 Jan 15. doi: 10.1038

Insert

Renin(mouse)-ECL-CD4-tevs-RXR6.2 (Ren1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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CA-0193 CMVTO-Renin(H2M)-ECL-CD4-tevs-RXR6.2

Plasmid

#231203

Purpose

ER-retained H2M renin

Depositor

Xiaojing Gao

Article

Aldrete et al Nat Chem Biol. 2025 Jan 15. doi: 10.1038

Insert

Renin(H2M)-ECL-CD4-tevs-RXR6.2 (REN Human)

Use

Tags

Expression

Mammalian

Mutation

L147I, R148H, I203V, L290V

Promoter

Available since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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CA-0234 CMVTO-sumIL2-rensGS.3

Plasmid

#231198

Purpose

CleaveIL-2

Depositor

Xiaojing Gao

Article

Aldrete et al Nat Chem Biol. 2025 Jan 15. doi: 10.1038

Insert

sumIL2-rensGS.3 (IL2 Human)

Use

Tags

Expression

Mammalian

Mutation

L80F, R81D, L85V, I86V, I92F

Promoter

Available since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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CA-0210 CMVTO-hIL2-rens-del.1

Plasmid

#231227

Purpose

CleaveIL-2_loop1_del

Depositor

Xiaojing Gao

Article

Aldrete et al Nat Chem Biol. 2025 Jan 15. doi: 10.1038

Insert

hIL2-rens-del.1 (IL2 Human)

Use

Tags

Expression

Mammalian

Mutation

L80F, R81D, L85V, I86V, I92F

Promoter

Available since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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CA-0211 CMVTO-hIL2-rens-del.2

Plasmid

#231228

Purpose

CleaveIL-2_loop3_del

Depositor

Xiaojing Gao

Article

Aldrete et al Nat Chem Biol. 2025 Jan 15. doi: 10.1038

Insert

hIL2-rens-del.2 (IL2 Human)

Use

Tags

Expression

Mammalian

Mutation

L80F, R81D, L85V, I86V, I92F

Promoter

Available since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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CA-0201 CMVTO-hIL6-rens.1

Plasmid

#231197

Purpose

CleaveIL-6

Depositor

Xiaojing Gao

Article

Aldrete et al Nat Chem Biol. 2025 Jan 15. doi: 10.1038

Insert

hIL6-rens.1 (IL6 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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CA-0212 CMVTO-hIL2-rens-del.3

Plasmid

#231229

Purpose

CleaveIL-2_loop2_del

Depositor

Xiaojing Gao

Article

Aldrete et al Nat Chem Biol. 2025 Jan 15. doi: 10.1038

Insert

hIL2-rens-del.3 (IL2 Human)

Use

Tags

Expression

Mammalian

Mutation

L80F, R81D, L85V, I86V, I92F

Promoter

Available since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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CA-0033 CMVTO-hIL6

Plasmid

#231192

Purpose

Human IL-6

Depositor

Xiaojing Gao

Article

Aldrete et al Nat Chem Biol. 2025 Jan 15. doi: 10.1038

Insert

Human IL-6 (IL6 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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